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Detection method for evaluating impact of transgenic cotton on dung beetles

A detection method and transgenic technology, applied in the application, animal feed, animal feed and other directions, can solve the problem of poor food selectivity, and achieve the effects of shortening the detection period, low cost and simple operation method.

Inactive Publication Date: 2013-03-13
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Dung beetle adults have chewing mouthparts and mainly feed on liquid food with high microbial content in cow dung and easily digestible plant residues. The diameter of solid food particles is generally less than 0.03mm, and its larvae are not very selective for food.

Method used

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  • Detection method for evaluating impact of transgenic cotton on dung beetles
  • Detection method for evaluating impact of transgenic cotton on dung beetles
  • Detection method for evaluating impact of transgenic cotton on dung beetles

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Experimental program
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Embodiment

[0022] At the end of August 2012, 200 g of cotton leaves from the transgenic Bt cotton variety (Zhong 30) and its parents (Zhong 16) were harvested and brought back to the laboratory. They were placed in the refrigerator at -10°C for 24 hours and then thoroughly pulverized. The particle diameter was less than 0.03mm. Evenly mix 40 g of crushed cotton leaves with 360 g of fresh cow dung; put 400 g of the mixed sample into a gauze bag with a mesh size of 0.2 mm, and then inoculate 8 captured dung beetles (wandering beetles) into the gauze bag , the gauze bag was sealed and placed on the outdoor surface; the number of beetle eggs, larvae and adults in the gauze bag were recorded on the 7th, 14th and 21st day respectively, and the egg laying rate, egg hatching rate and mortality were calculated.

[0023] The results showed that the oviposition rate of beetles in Zhong30 treatment was significantly lower than that of Zhong16 ( figure 1 ); there was no significant difference in egg...

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Abstract

The invention belongs to the technical field of ecological risk assessment of transgenic crops, in particular to a detection method for evaluating the impact of transgenic cotton on dung beetles. The method is realized through the following steps: by taking Bt transgenic insect-resistant cotton and parents thereof and dung beetles as detection objects, collecting fresh cotton leaves, freezing the cotton leaves at -10 DEG C for 24 hours in a refrigerator, and crushing until the particle diameter is less than 0.03 mm; weighing the crushed cotton leaves and cow dung, and mixing proportionally and thoroughly; loading the resulting mixture into a gauze bag which has a pore size of 0.2 mm, inoculating dung beetles to the gauze bag, and sealing the gauze bag and placing on the ground outdoor; and recording the numbers of beetle eggs, larvae and adult insects on the 7th day, the 14th day and the 21st day respectively, and calculating the egg-laying rate, hatching rate and adult insect death rate. The death rate of adult beetles treated by the Bt transgenic insect-resistant cotton is increased, and the egg-laying rate and the hatching rate are reduced, which indicates that the Bt transgenic insect-resistant cotton has a harmful effect on dung beetles. The detection method is accurate, rapid, low in cost and convenient to apply.

Description

technical field [0001] The invention belongs to the technical field of ecological risk assessment of transgenic crops, and in particular relates to a detection method for evaluating the impact of transgenic Bt cotton on dung beetles. Background technique [0002] At present, transgenic technology has been widely used in the field of agriculture and has significantly improved crop yields. However, because it is different from traditional crop breeding, it is difficult for people to predict the consequences of genes entering a new genetic background. Therefore, while paying attention to the benefits of genetically modified crops, scientists from various countries have also been concerned about their potential ecological risks. [0003] In 1987, Vaeck reported for the first time that the Bt insecticidal protein gene was transferred into cotton, which opened the era of large-scale planting of transgenic cotton. Up to now, the planting area of ​​transgenic insect-resistant cotto...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K1/18A23K1/14
Inventor 孙书存吴新卫曹瑞
Owner NANJING UNIV
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