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Application and product of dsRNA in potato beetle control

A potato beetle and insecticide technology, applied in the direction of DNA / RNA fragments, application, recombinant DNA technology, etc., can solve the problems of high production cost, immature dsRNA technology, instability, etc., and achieve low cost, effective and cheap Uniform, fast-acting effect

Pending Publication Date: 2019-06-21
INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current RNAi technology still has obvious shortcomings: ① The dsRNA or siRNA that triggers RNAi is easily degraded by nucleases ubiquitous in the environment, so it is unstable; ② The dsRNA technology for production and storage is not yet mature, and the production cost is high

Method used

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  • Application and product of dsRNA in potato beetle control
  • Application and product of dsRNA in potato beetle control
  • Application and product of dsRNA in potato beetle control

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0030] Embodiment 1: The application of dsLdalpha snap in the control of potato beetles in this embodiment, wherein the nucleotide sequence of dsLdalpha snap is shown in SEQ ID NO:1.

specific Embodiment approach 2

[0031] Embodiment 2: The difference between this embodiment and Embodiment 1 is that dsLdalpha snap is used as the active ingredient of the potato beetle insecticide. Others are the same as the first embodiment.

specific Embodiment approach 3

[0032] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the bacteria solution containing the genetically engineered bacteria expressing dsLdalpha snap is used as the potato beetle insecticide. Others are the same as the first or second embodiment.

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Abstract

The invention discloses an application and a product of dsRNA in potato beetle control, and relates to an application of a gene product in potato beetle control and a derivative product. The dsRNA product has strong stability, mature production technology and low cost, and is an effective cheap 'gene pesticide' product with strong specificity, green and environmental protection. The application ofthe dsRNA product in potato beetle control has great and far-reaching significance in potato beetle control. After a potato beetle pesticide is sprayed on leaves of potato plants at a low dose, the potted potato plants can continuously resist insects for more than 28 days, and the control effect reaches more than 80%; and under the outdoor strong ultraviolet condition, the potato beetle pesticidestill has a strong poisoning effect on potato beetles, the field prevention and control lasting period is about 3 weeks, and the prevention and control effect is gradually reduced from 50% to about 10%.

Description

technical field [0001] The invention relates to the application of a gene product in the control of potato beetle and its derivative product. Background technique [0002] Potato is an important grain, vegetable, feed crop and industrial raw material crop in the world. In recent years, due to its obvious comparative economic benefits and other characteristics, the planting area in my country has continued to increase, and it has become the first in my country after rice, wheat and corn. 4 major staple food crops. The potato beetle, Leptinotarsa ​​decemlineata, also known as the potato beetle, its English name Colorado Potato Beetle (CPB), belongs to the order Coleoptera and the Chrysolithidae. It is recognized as a destructive pest of potatoes worldwide, and it is also one of the major quarantine targets in my country. Its host range is relatively narrow, and it mainly harms more than 20 species of Solanaceae plants such as potatoes, eggplants, cultivated tomatoes, Heliconia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/70C12N1/21A01N57/16A01P7/04C12R1/19
Inventor 郭文超付开赟丁新华吐尔逊·阿合买提何江王小武班小莉刘文黄红梅王娜
Owner INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
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