Microbial agent for inhibiting root-knot nematode and preparation method thereof

Abstract

The invention relates to the technical field of microbial agents. The invention provides a microbial agent for inhibiting root-knot nematode and a preparation method thereof. The microbial agent is prepared from the following raw materials in parts by weight: 6-10 parts of paecilomyces lilacinus liquid, 5-7 parts of bacillus thuringiensis liquid, 6-8 parts of beauveria bassiana culture, 6-10 parts of bacillus subtilis liquid and 50-60 parts of a carrier. The microbial agent disclosed by the invention can effectively prevent and treat root-knot nematodes, reduce the influence of the root-knot nematodes on crops, and improve the soil fertility at the same time. The effects of biologically controlling insects, biologically fertilizing soil and promoting plant growth are achieved, and the yield and quality of crops are improved.

Description

Technical Field

[0001] The invention relates to the technical field of microbial agents, and in particular to a microbial agent for inhibiting root-knot nematodes and a preparation method thereof. Background Art

[0002] Root-knot nematodes are a highly specialized omnivorous plant pathogenic nematode. They have a wide range of hosts and can harm more than 130 crops from 39 families. Nematodes are very small and difficult to see with the naked eye. They are mostly distributed in the soil of 0 to 20 cm, especially in the soil of 3 to 9 cm where the largest number of nematodes are found. They are dioecious, with female adults being pear-shaped and male adults being thread-shaped. They often overwinter as eggs or second-instar larvae left in the soil or manure with plant debris, or invade through tender roots as second-instar larvae the following year when the environment is suitable, and reproduce and cause harm. Nematodes can be spread through worm-carrying soil or seedlings and irrigation water. Nematodes reproduce very quickly when the soil temperature is 25℃ to 30℃ and the soil moisture is 40%-70%.

[0003] Root-knot nematodes mainly harm the roots of various vegetables, which are manifested by an increase in lateral roots and fibrous roots compared to normal, and the formation of spherical or conical white nodules of varying sizes on the fibrous roots of young roots, some of which are bead-shaped. The aboveground part of the affected plants grows short and slow, with abnormal leaf color, few fruits, low yields, and even premature death of the plants. Therefore, it is very important to develop green drugs related to root-knot nematodes. Summary of the invention

[0004] The purpose of the present invention is to provide a microbial agent for inhibiting root-knot nematodes and a preparation method thereof, so as to effectively prevent and control the influence of root-knot nematodes on crops and improve soil fertility, thereby achieving biological pest control, biological fertilization, promoting plant growth, and improving crop yield and quality.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a bacterial agent for inhibiting root-knot nematodes. The bacterial agent is prepared from the following raw materials in parts by weight: 6-10 parts of Paecilomyces lilacinus liquid, 5-7 parts of Bacillus thuringiensis liquid, 6-8 parts of Beauveria bassiana culture, 6-10 parts of Bacillus subtilis liquid and 50-60 parts of a carrier.

[0007] Preferably, the preparation method of the Paecilomyces lilacinus liquid is as follows: inoculating the activated Paecilomyces lilacinus strain into a Paecilomyces lilacinus culture medium, and culturing at 25 to 28° C. for 60 to 84 hours to obtain the Paecilomyces lilacinus liquid;

[0008] The formula of the Paecilomyces lilacinus culture medium is: 180-190 g / L potato, 8-12 g / L peptone, 20-22 g / L sucrose, 6-8 g / L sodium nitrate, 0.2-0.4 g / L iron sulfate, and the balance is water;

[0009] The pH value of the Paecilomyces lilacinus culture medium is 6.2-6.5;

[0010] During the culture process, the culture medium is ventilated, and the amount of ventilation is 100-110 m 3 / h.

[0011] Preferably, the preparation method of the Bacillus thuringiensis bacterial solution is: inoculating the activated Bacillus thuringiensis strain into a Bacillus thuringiensis culture medium, and culturing at 30 to 32° C. for 48 to 72 hours to obtain the Bacillus thuringiensis bacterial solution;

[0012] The formula of the Bacillus thuringiensis culture medium is: 40-80 g / L molasses, 1-2 g / L yeast extract, 20-50 g / L malt extract, 20-50 g / L corn steep liquor, 0.15-0.2 g / L magnesium sulfate, and the balance is water.

[0013] Preferably, the preparation method of the Beauveria bassiana culture is: inoculating the activated Beauveria bassiana strain into a Beauveria bassiana culture medium, and culturing at 30 to 32° C. for 40 to 50 hours to obtain the Beauveria bassiana culture;

[0014] The formula of the Beauveria bassiana culture medium is: 30-35 parts of potato flour, 30-35 parts of corn flour, 60-65 parts of wheat bran, 6-8 parts of urea, and 12-14 parts of sucrose.

[0015] Preferably, the preparation method of the Bacillus subtilis bacterial liquid is: inoculating the activated Bacillus subtilis strain into a Bacillus subtilis culture medium, and culturing at 28 to 30° C. for 60 to 70 hours to obtain the Bacillus subtilis bacterial liquid;

[0016] The formula of the Bacillus subtilis culture medium is: 25-30 g / L glucose, 18-22 g / L beef extract, 8-10 g / L peptone, 25-35 g / L starch, 1-3 g / L sodium chloride, and the balance is water;

[0017] The pH of the Bacillus subtilis culture medium is 6.8-7.2.

[0018] Preferably, the type of Beauveria bassiana is Beauveria bassiana or Beauveria bassiana, and the type of the carrier is rice husk or soybean meal.

[0019] The present invention also provides a method for preparing the bacterial agent, comprising the following steps: mixing a Paecilomyces lilacinus solution, a Bacillus thuringiensis solution, a Beauveria bassiana culture, a Bacillus subtilis solution and a carrier, standing the mixture at 20 to 30° C. for 24 to 36 hours, and drying the mixture to obtain the bacterial agent.

[0020] The invention also provides application of the bacterial agent in preventing and controlling root-knot nematodes.

[0021] The present invention also provides a method for controlling root-knot nematodes by using the bacterial agent, comprising the following steps: applying the bacterial agent to the soil at an amount of 10 to 25 kg / mu before sowing.

[0022] Preferably, the microbial agent can be used in combination with conventional fertilizers and can be mixed with conventional fertilizers and then applied to the soil.

[0023] The present invention provides a microbial agent for inhibiting root-knot nematodes and a preparation method thereof. The microbial agent is prepared from the following raw materials in parts by weight: 6 to 10 parts of Paecilomyces lilacinus liquid, 5 to 7 parts of Bacillus thuringiensis liquid, 6 to 8 parts of Beauveria bassiana culture, 6 to 10 parts of Bacillus subtilis liquid, and 50 to 60 parts of a carrier. The microbial agent of the present invention can effectively prevent and control root-knot nematodes, reduce the impact of root-knot nematodes on crops, and improve soil fertility. It realizes biological pest control, biological fertilization, and the effects of promoting plant growth, thereby improving the yield and quality of crops. DETAILED DESCRIPTION

[0024] The invention provides a bacterial agent for inhibiting root-knot nematodes. The bacterial agent comprises the following raw materials in parts by weight: 6-10 parts of Paecilomyces lilacinus liquid, 5-7 parts of Bacillus thuringiensis liquid, 6-8 parts of Beauveria bassiana culture, 6-10 parts of Bacillus subtilis liquid and 50-60 parts of a carrier.

[0025] In the present invention, the number of parts of the Paecilomyces lilacinus solution is preferably 7 to 9 parts, and more preferably 8 parts.

[0026] In the present invention, the amount of the Bacillus thuringiensis bacterial solution is preferably 5.5 to 6.5 parts, more preferably 6 parts.

[0027] In the present invention, the number of portions of the Beauveria bassiana culture is preferably 6.5 to 7.5 portions, and more preferably 7 portions.

[0028] In the present invention, the number of parts of the Bacillus subtilis liquid is preferably 7 to 9 parts, and more preferably 8 parts.

[0029] In the present invention, the amount of the carrier is preferably 52 to 58 parts, more preferably 55 parts.

[0030] In the present invention, the preparation method of the Paecilomyces lilacinus liquid is preferably: inoculating the activated Paecilomyces lilacinus strain into a Paecilomyces lilacinus culture medium, culturing at 25-28°C for 60-84h, and drying to obtain the Paecilomyces lilacinus liquid, and further preferably: inoculating the activated Paecilomyces lilacinus strain into a Paecilomyces lilacinus culture medium, culturing at 26-27°C for 72h, and drying to obtain the Paecilomyces lilacinus liquid;

[0031] The formula of the Paecilomyces lilacinus culture medium is preferably: 180-190 g / L potato, 8-12 g / L peptone, 20-22 g / L sucrose, 6-8 g / L sodium nitrate, 0.2-0.4 g / L iron sulfate, and the balance is water, and more preferably: 185 g / L potato, 10 g / L peptone, 21 g / L sucrose, 7 g / L sodium nitrate, 0.3 g / L iron sulfate, and the balance is water;

[0032] The pH value of the Paecilomyces lilacinus culture medium is preferably 6.2 to 6.5, and more preferably 6.3 to 6.4;

[0033] The culture medium is preferably ventilated during the culture process, and the amount of ventilation is preferably 100 to 110 m 3 / h, more preferably 105m 3 / h.

[0034] In the present invention, the preparation method of the Bacillus thuringiensis bacterial liquid is: inoculating the activated Bacillus thuringiensis strain into the Bacillus thuringiensis culture medium, culturing at 30-32°C for 48-72h, and obtaining the Bacillus thuringiensis bacterial liquid. More preferably, the activated Bacillus thuringiensis strain is inoculated into the Bacillus thuringiensis culture medium, culturing at 31°C for 60h, and obtaining the Bacillus thuringiensis bacterial liquid.

[0035] The formula of the Bacillus thuringiensis culture medium is preferably: 40-80 g / L molasses, 1-2 g / L yeast extract, 20-50 g / L malt extract, 20-50 g / L corn steep liquor, 0.15-0.2 g / L magnesium sulfate, and the balance is water, and is further preferably: 60 g / L molasses, 1.5 g / L yeast extract, 35 g / L malt extract, 35 g / L corn steep liquor, 0.17-0.18 g / L magnesium sulfate, and the balance is water.

[0036] In the present invention, the method for preparing the Beauveria culture is preferably: inoculating the activated Beauveria strain into the Beauveria culture medium, culturing at 30-32°C for 40-50h to obtain the Beauveria culture, and further preferably: inoculating the activated Beauveria strain into the Beauveria culture medium, culturing at 31°C for 45h to obtain the Beauveria culture;

[0037] The formula of the Beauveria bassiana culture medium is preferably: 30-35 parts of potato flour, 30-35 parts of corn flour, 60-65 parts of wheat bran, 6-8 parts of urea, and 12-14 parts of sucrose, and more preferably: 32-33 parts of potato flour, 32-33 parts of corn flour, 63 parts of wheat bran, 7 parts of urea, and 13 parts of sucrose.

[0038] In the present invention, the preparation method of the Bacillus subtilis liquid is preferably: inoculating the activated Bacillus subtilis strain into a Bacillus subtilis culture medium, culturing at 28 to 30° C. for 60 to 70 hours, and drying to obtain the Bacillus subtilis liquid. It is further preferably: inoculating the activated Bacillus subtilis strain into a Bacillus subtilis culture medium, culturing at 29° C. for 65 hours, and drying to obtain the Bacillus subtilis liquid.

[0039] The formula of the Bacillus subtilis culture medium is preferably: 25-30 g / L glucose, 18-22 g / L beef extract, 8-10 g / L peptone, 25-35 g / L starch, 1-3 g / L sodium chloride, and the balance is water, and more preferably: 27-18 g / L glucose, 20 g / L beef extract, 9 g / L peptone, 30 g / L starch, 2 g / L sodium chloride, and the balance is water;

[0040] The pH of the Bacillus subtilis culture medium is preferably 6.8 to 7.2, and more preferably 7.0.

[0041] In the present invention, the type of Beauveria bassiana is preferably Beauveria bassiana or Beauveria brandelli, and the type of the carrier is preferably rice husk or soybean meal.

[0042] The present invention also provides a preparation method of the bacterial agent, comprising the following steps: mixing a Paecilomyces lilacinus solution, a Bacillus thuringiensis solution, a Beauveria bassiana culture, a Bacillus subtilis solution and a carrier, standing for 24 to 36 hours at 20 to 30° C., and drying to obtain the bacterial agent. More preferably, the Paecilomyces lilacinus solution, the Bacillus thuringiensis solution, the Beauveria bassiana culture, the Bacillus subtilis solution and a carrier are mixed, standing for 30 hours at 25° C., and drying to obtain the bacterial agent.

[0043] The invention also provides application of the bacterial agent in preventing and controlling root-knot nematodes.

[0044] The present invention also provides a method for controlling root-knot nematodes using the bacterial agent, preferably comprising the following steps: applying 10 to 25 kg / mu to the soil before sowing, and more preferably: applying 15 to 20 kg / mu to the soil before sowing.

[0045] In the present invention, the bacterial agent can be used in combination with conventional fertilizers, and can be mixed with conventional fertilizers and then applied to the bottom.

[0046] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0047] Example 1

[0048] A method for preparing a microbial agent for inhibiting root-knot nematodes comprises the following steps:

[0049] (1) The activated Paecilomyces lilacinus strain was inoculated into the Paecilomyces lilacinus culture medium and cultured at 25°C for 84 h. During the culture process, the temperature was adjusted according to 100 m 3 / h standard to aerate the culture medium to obtain the Paecilomyces lilacinus solution;

[0050] (2) inoculating the activated Bacillus thuringiensis strain into a Bacillus thuringiensis culture medium, and culturing at 30° C. for 72 hours to obtain the Bacillus thuringiensis bacterial solution;

[0051] (3) inoculating the activated Beauveria bassiana strain into a Beauveria bassiana culture medium, and culturing at 30° C. for 50 hours to obtain the Beauveria bassiana culture;

[0052] (4) inoculating the activated Bacillus subtilis strain into a Bacillus subtilis culture medium, and culturing at 28° C. for 70 hours to obtain the Bacillus subtilis culture solution;

[0053] (5) weighing raw materials according to the weight ratio of 6 parts of Paecilomyces lilacinus liquid, 7 parts of Bacillus thuringiensis liquid, 6 parts of Beauveria bassiana culture, 6 parts of Bacillus subtilis liquid, and 60 parts of rice husk, mixing the Paecilomyces lilacinus liquid, Bacillus thuringiensis liquid, Beauveria bassiana culture, Bacillus subtilis liquid and rice husk, standing at 20° C. for 36 hours, and drying to obtain the bacterial agent;

[0054] Note: In this example, the formula of the culture medium of Paecilomyces lilacinus is: 180 g / L potato, 12 g / L peptone, 22 g / L sucrose, 8 g / L sodium nitrate, 0.2 g / L iron sulfate, the balance is water, pH = 6.2;

[0055] The formula of Bacillus thuringiensis culture medium is: molasses 40g / L, yeast extract 1g / L, malt extract 50g / L, corn steep liquor 20g / L, magnesium sulfate 0.2g / L, and the balance is water;

[0056] The formula of the Beauveria bassiana culture medium is: 35 parts of potato flour, 35 parts of corn flour, 60 parts of wheat bran, 6 parts of urea, and 12 parts of sucrose;

[0057] The formula of the Bacillus subtilis culture medium is: 25 g / L glucose, 22 g / L beef extract, 8 g / L peptone, 35 g / L starch, 1 g / L sodium chloride, the balance is water, and the pH is 7.2.

[0058] Example 2

[0059] A method for preparing a microbial agent for inhibiting root-knot nematodes comprises the following steps:

[0060] (1) The activated Paecilomyces lilacinus strain was inoculated into the Paecilomyces lilacinus culture medium and cultured at 28°C for 60 h. During the culture process, the temperature was kept at 110 °C. 3 / h standard to aerate the culture medium to obtain the Paecilomyces lilacinus solution;

[0061] (2) inoculating the activated Bacillus thuringiensis strain into a Bacillus thuringiensis culture medium, and culturing at 32° C. for 48 hours to obtain the Bacillus thuringiensis bacterial solution;

[0062] (3) inoculating the activated Beauveria bassiana strain into a Beauveria bassiana culture medium, and culturing at 32° C. for 40 hours to obtain the Beauveria bassiana culture;

[0063] (4) inoculating the activated Bacillus subtilis strain into a Bacillus subtilis culture medium, and culturing at 30° C. for 60 hours to obtain the Bacillus subtilis culture solution;

[0064] (5) weighing raw materials according to the weight ratio of 10 parts of Paecilomyces lilacinus liquid, 5 parts of Bacillus thuringiensis liquid, 8 parts of Beauveria bassiana culture, 10 parts of Bacillus subtilis liquid, and 50 parts of soybean meal, mixing the Paecilomyces lilacinus liquid, Bacillus thuringiensis liquid, Beauveria bassiana culture, Bacillus subtilis liquid and soybean meal, standing at 30° C. for 24 hours, and drying to obtain the bacterial agent;

[0065] Note: In this example, the formula of the culture medium for Paecilomyces lilacinus is: 190 g / L potato, 8 g / L peptone, 20 g / L sucrose, 6 g / L sodium nitrate, 0.4 g / L iron sulfate, the balance is water, pH = 6.5;

[0066] The formula of Bacillus thuringiensis culture medium is: molasses 80g / L, yeast extract 2g / L, malt extract 20g / L, corn steep liquor 50g / L, magnesium sulfate 0.15g / L, and the balance is water;

[0067] The formula of the Beauveria bassiana culture medium is: 30 parts of potato flour, 30 parts of corn flour, 65 parts of wheat bran, 8 parts of urea, and 14 parts of sucrose;

[0068] The formula of the Bacillus subtilis culture medium is: 30 g / L glucose, 18 g / L beef extract, 10 g / L peptone, 25 g / L starch, 3 g / L sodium chloride, the balance is water, and the pH is 6.8.

[0069] Example 3

[0070] A method for preparing a microbial agent for inhibiting root-knot nematodes comprises the following steps:

[0071] (1) The activated Paecilomyces lilacinus strain was inoculated into the Paecilomyces lilacinus culture medium and cultured at 26°C for 72 h. During the culture process, the temperature was adjusted to 105 m 3 / h standard to aerate the culture medium to obtain the Paecilomyces lilacinus solution;

[0072] (2) inoculating the activated Bacillus thuringiensis strain into a Bacillus thuringiensis culture medium, and culturing at 31° C. for 60 hours to obtain the Bacillus thuringiensis bacterial solution;

[0073] (3) inoculating the activated Beauveria bassiana strain into a Beauveria bassiana culture medium, and culturing at 31° C. for 45 hours to obtain the Beauveria bassiana culture;

[0074] (4) inoculating the activated Bacillus subtilis strain into a Bacillus subtilis culture medium, and culturing at 29° C. for 65 hours to obtain the Bacillus subtilis culture solution;

[0075] (5) weighing raw materials according to the weight ratio of 8 parts of Paecilomyces lilacinus liquid, 6 parts of Bacillus thuringiensis liquid, 7 parts of Beauveria bassiana culture, 8 parts of Bacillus subtilis liquid, and 55 parts of rice husk, mixing the Paecilomyces lilacinus liquid, Bacillus thuringiensis liquid, Beauveria bassiana culture, Bacillus subtilis liquid and rice husk, standing at 25° C. for 30 hours, and drying to obtain the bacterial agent;

[0076] Note: In this example, the formula of the culture medium for Paecilomyces lilacinus is: 185 g / L potato, 10 g / L peptone, 21 g / L sucrose, 7 g / L sodium nitrate, 0.3 g / L iron sulfate, and the balance is water, pH = 6.3;

[0077] The formula of Bacillus thuringiensis culture medium is: molasses 60g / L, yeast extract 1.5g / L, malt extract 35g / L, corn steep liquor 35g / L, magnesium sulfate 0.17g / L, and the balance is water;

[0078] The formula of the Beauveria bassiana culture medium is: 33 parts of potato flour, 33 parts of corn flour, 62 parts of wheat bran, 7 parts of urea, and 13 parts of sucrose;

[0079] The formula of the Bacillus subtilis culture medium is: 27 g / L glucose, 20 g / L beef extract, 9 g / L peptone, 30 g / L starch, 2 g / L sodium chloride, the balance is water, and the pH is 7.0.

[0080] Comparative Example 1

[0081] A method for preparing a microbial agent for inhibiting root-knot nematodes comprises the following steps:

[0082] (1) The activated Paecilomyces lilacinus strain was inoculated into the Paecilomyces lilacinus culture medium and cultured at 26°C for 72 h. During the culture process, the temperature was adjusted to 105 m 3 / h standard to aerate the culture medium to obtain the Paecilomyces lilacinus solution;

[0083] (2) inoculating the activated Beauveria bassiana strain into a Beauveria bassiana culture medium, and culturing at 31° C. for 45 hours to obtain the Beauveria bassiana culture;

[0084] (3) weighing raw materials according to the weight ratio of 14 parts of Paecilomyces lilacinus liquid, 15 parts of Beauveria bassiana culture, and 55 parts of rice husk, mixing the Paecilomyces lilacinus liquid, Beauveria bassiana culture, and rice husk, standing at 25° C. for 30 hours, and drying to obtain the bacterial agent;

[0085] Note: In this example, the formula of the culture medium for Paecilomyces lilacinus is: 185 g / L potato, 10 g / L peptone, 21 g / L sucrose, 7 g / L sodium nitrate, 0.3 g / L iron sulfate, and the balance is water, pH = 6.3;

[0086] The formula of the Beauveria bassiana culture medium is: 33 parts of potato flour, 33 parts of corn flour, 62 parts of wheat bran, 7 parts of urea, and 13 parts of sucrose.

[0087] Comparative Example 2

[0088] A method for preparing a microbial agent for inhibiting root-knot nematodes comprises the following steps:

[0089] (1) The activated Paecilomyces lilacinus strain was inoculated into the Paecilomyces lilacinus culture medium and cultured at 26°C for 72 h. During the culture process, the temperature was adjusted to 105 m 3 / h standard to aerate the culture medium to obtain the Paecilomyces lilacinus solution;

[0090] (2) inoculating the activated Bacillus thuringiensis strain into a Bacillus thuringiensis culture medium, and culturing at 31° C. for 60 hours to obtain the Bacillus thuringiensis bacterial solution;

[0091] (3) inoculating the activated Beauveria bassiana strain into a Beauveria bassiana culture medium, and culturing at 31° C. for 45 hours to obtain the Beauveria bassiana culture;

[0092] (4) inoculating the activated Bacillus subtilis strain into a Bacillus subtilis culture medium, and culturing at 29° C. for 65 hours to obtain the Bacillus subtilis culture solution;

[0093] (5) weighing raw materials according to the weight ratio of 8 parts of Paecilomyces lilacinus liquid, 6 parts of Bacillus thuringiensis liquid, 7 parts of Beauveria bassiana culture, 8 parts of Bacillus subtilis liquid, and 55 parts of rice husk, mixing the Paecilomyces lilacinus liquid, Bacillus thuringiensis liquid, Beauveria bassiana culture, Bacillus subtilis liquid and rice husk, standing at 25° C. for 30 hours, and drying to obtain the bacterial agent;

[0094] Note: In this embodiment, the formula of the Paecilomyces lilacinus culture medium, Bacillus thuringiensis culture medium and Bacillus subtilis culture medium is: 185 g / L potato, 10 g / L peptone, 21 g / L sucrose, 7 g / L sodium nitrate, 0.3 g / L iron sulfate, the balance is water, pH = 6.3;

[0095] The formula of the Beauveria bassiana culture medium is: 33 parts of potato flour, 33 parts of corn flour, 62 parts of wheat bran, 7 parts of urea, and 13 parts of sucrose.

[0096] Test example

[0097] A planting experiment was conducted in a 60-mu test field on April 23, 2022. Before the experiment began, the test field was divided into six plots, each with an area of ​​10 mu. They were numbered as experimental groups 1 to 3 and control groups 1 to 3.

[0098] Before sowing, apply base fertilizer. When applying base fertilizer, mix the microbial agent and base fertilizer and apply them at one time, and turn the soil after application. Among them, 20 kg of the microbial agent prepared in Example 1 was applied to the test field of test group 1, 20 kg of the microbial agent prepared in Example 2 was applied to the test field of test group 2, and 20 kg of the microbial agent prepared in Example 3 was applied to the test field of test group 3; 20 kg of the microbial agent prepared in comparative example 1 was applied to the test field of control group 1, 20 kg of the microbial agent prepared in comparative example 2 was applied to the test field of control group 2, and 20 kg of rice husk was applied to the test field of control group 3 as a blank control. The type of base fertilizer, the amount of base fertilizer and the method of fertilization used in each group are exactly the same.

[0099] Soybean sowing was carried out after applying base fertilizer. Dongnong 47 soybeans were used for sowing. The soybean sowing amount for each group was the same. After sowing until harvest, each group carried out routine planting management according to the same method.

[0100] After harvest, the soybean yield changes, the number of cysts, eggs and second-instar larvae of soybean cyst nematodes in each group were statistically analyzed with the control group 3 as the benchmark. The results are shown in Table 1.

[0101] Table 1

[0102] Group Changes in soybean production Changes in the number of cysts Changes in egg number Changes in the number of second-instar larvae Experimental Group 1 +34.76％ -31.08％ -48.19％ -27.33％ Experimental Group 2 +33.89％ -30.56％ -49.03％ -28.36％ Experimental Group 3 +37.07％ -32.01％ -49.12％ -27.18％ Comparative Example 1 +10.29％ -17.08％ -21.85％ -14.09％ Comparative Example 2 +15.81％ -20.11％ -24.62％ -18.11％

[0103] Conclusion: The microbial agent of the present invention has a reasonable formula and significant effect; it can significantly inhibit root-knot nematodes, increase crop yield, and improve crop yield and quality.

[0104] As can be seen from the above embodiments, the present invention provides a bacterial agent for inhibiting root-knot nematodes and a preparation method thereof, wherein the bacterial agent is prepared from the following raw materials in parts by weight: 6 to 10 parts of Paecilomyces lilacinus liquid, 5 to 7 parts of Bacillus thuringiensis liquid, 6 to 8 parts of Beauveria bassiana culture, 6 to 10 parts of Bacillus subtilis liquid, and 50 to 60 parts of a carrier. The bacterial agent of the present invention can effectively prevent and control root-knot nematodes, reduce the impact of root-knot nematodes on crops, and improve soil fertility. It realizes biological pest control, biological fertile soil, and the effects of promoting plant growth, thereby improving the yield and quality of crops.

[0105] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A fungal agent for inhibiting root-knot nematodes, characterized in that: The bacterial agent is prepared from the following raw materials in parts by weight: 6-10 parts of Paecilomyces lilacinus liquid, 5-7 parts of Bacillus thuringiensis liquid, 6-8 parts of Beauveria bassiana culture, 6-10 parts of Bacillus subtilis liquid and 50-60 parts of carrier.

2. The bacterial agent according to claim 1, characterized in that The preparation method of the Paecilomyces lilacinus liquid is as follows: inoculating the activated Paecilomyces lilacinus strain into a Paecilomyces lilacinus culture medium, and culturing at 25 to 28° C. for 60 to 84 hours to obtain the Paecilomyces lilacinus liquid; The formula of the Paecilomyces lilacinus culture medium is: 180-190 g / L potato, 8-12 g / L peptone, 20-22 g / L sucrose, 6-8 g / L sodium nitrate, 0.2-0.4 g / L iron sulfate, and the balance is water; The pH value of the Paecilomyces lilacinus culture medium is 6.2-6.5; During the culture process, the culture medium is ventilated, and the amount of ventilation is 100-110 m 3 / h.

3. The bacterial agent according to claim 1, characterized in that The preparation method of the Bacillus thuringiensis bacterial liquid is as follows: inoculating the activated Bacillus thuringiensis strain into a Bacillus thuringiensis culture medium, and culturing at 30 to 32° C. for 48 to 72 hours to obtain the Bacillus thuringiensis bacterial liquid; The formula of the Bacillus thuringiensis culture medium is: 40-80 g / L molasses, 1-2 g / L yeast extract, 20-50 g / L malt extract, 20-50 g / L corn steep liquor, 0.15-0.2 g / L magnesium sulfate, and the balance is water.

4. The bacterial agent according to claim 1, characterized in that The preparation method of the Beauveria bassiana culture comprises: inoculating the activated Beauveria bassiana strain into a Beauveria bassiana culture medium, and culturing at 30 to 32° C. for 40 to 50 hours to obtain the Beauveria bassiana culture; The formula of the Beauveria bassiana culture medium is: 30-35 parts of potato flour, 30-35 parts of corn flour, 60-65 parts of wheat bran, 6-8 parts of urea, and 12-14 parts of sucrose.

5. The bacterial agent according to claim 1, characterized in that The preparation method of the Bacillus subtilis bacterial liquid is as follows: inoculating the activated Bacillus subtilis strain into a Bacillus subtilis culture medium, and culturing at 28 to 30° C. for 60 to 70 hours to obtain the Bacillus subtilis bacterial liquid; The formula of the Bacillus subtilis culture medium is: 25-30 g / L glucose, 18-22 g / L beef extract, 8-10 g / L peptone, 25-35 g / L starch, 1-3 g / L sodium chloride, and the balance is water; The pH of the Bacillus subtilis culture medium is 6.8-7.

2.

6. The bacterial agent according to claim 1, characterized in that The type of Beauveria bassiana is Beauveria bassiana or Beauveria brucei, and the type of the carrier is rice husk or soybean meal.

7. The method for preparing the bacterial agent according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: mixing Paecilomyces lilacinus liquid, Bacillus thuringiensis liquid, Beauveria bassiana culture, Bacillus subtilis liquid and a carrier, standing at 20-30°C for 24-36 hours, and drying to obtain the bacterial agent.

8. Use of the bacterial agent according to any one of claims 1 to 6 in controlling root-knot nematodes.

9. A method for controlling root-knot nematodes using the bacterial agent according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: applying 10-25 kg / mu of the fertilizer into the soil before sowing.

10. The method according to claim 9, characterized in that The microbial agent can be used in combination with conventional fertilizers and can be mixed with conventional fertilizers and then applied to the soil.