Inhibition effect of paris polyphylla peel extracting solution on plant pathogenic fungi

Abstract

The invention relates to the field of plant preparations, in particular to an inhibiting effect of a paris polyphylla pericarp extracting solution on plant pathogenic fungi, and a preparation method of the paris polyphylla pericarp extracting solution comprises the following steps: drying paris polyphylla pericarp, grinding into powder, adding methanol into the powder, carrying out ultrasonic dissolution and centrifugation to obtain supernate, and filtering to obtain the paris polyphylla pericarp extracting solution. Drying the supernate under reduced pressure, fully dissolving with distilled water to obtain a solution, extracting the solution with n-butyl alcohol, drying under reduced pressure again to obtain a concentrated product, and adding methanol into the concentrated product for constant volume to obtain a paris polyphylla peel extracting solution; the plant pathogenic fungi comprise fusarium graminearum, alternaria kikuchiana, colletotrichum brassicae, towel gourd fusarium wilt and sclerotinia sclerotiorum. The paris polyphylla pericarp extracting solution provided by the invention has very ideal inhibition effects on five plant pathogenic fungi, namely fusarium graminearum, alternaria kirilowii, colletotrichum brassicae, towel gourd fusarium wilt and sclerotinia sclerotiorum, and provides a solid theoretical basis for preparing a novel botanical fungicide.

Description

Technical Field

[0001] The invention relates to the field of plant preparations, in particular to the inhibitory effect of a lycopodiella cernua peel extract on plant pathogenic fungi. Background Art

[0002] Plant pathogenic fungal infection is a global agricultural problem. At present, about 80% of plant diseases are caused by fungal infection, which has a serious impact on the growth and development of plants and widely harms various plants. Common and serious diseases in agricultural production include: 1. Wheat fusarium ergot, which is caused by infection with Fusarium graminearum. Due to multiple factors such as climate and environment, the disease is mainly distributed in humid and semi-humid areas. In addition, with the changes in climate, agricultural production methods and wheat varieties, wheat fusarium ergot has gradually spread northward and its impact has expanded. 2. Pear black spot is one of the important diseases in my country's pear producing areas caused by infection with Alternaria alternate (Fr.) Keissl. It mainly harms the leaves, fruits and new shoots of pears, especially the tender leaves are very susceptible to the disease. The occurrence of pear black spot disease generally begins in late April, and spots will appear on the leaves. If it rains in June-July, the disease will be more serious. 3. Chinese cabbage anthracnose is the main disease that harms Chinese cabbage. It is mainly caused by infection with Colletotrichum higginsianum. When the temperature is higher than 20℃ and the relative humidity is above 80%, it is very easy to cause large-scale occurrence of Chinese cabbage anthracnose. 4. Luffa wilt is caused by infection with Fusarium oxysporum (Schl.) f.sp.1uffae (Kwai) Suzki et Kawai. This pathogen can evolve into a new physiological race and overwinter in the soil with sclerotia or thick-walled spores. It has strong saprophytic properties and can survive in the soil for more than 5 years, making the prevention and control of luffa wilt very difficult. 5. Rape sclerotinia is caused by rapeseed infection with Sclerotinia sclerotiorum (Lib.) de Bary. It is a common disease in rapeseed producing areas. It is highly infectious and can infect rapeseed, cucumber, soybean, sunflower, tobacco and other plants. Rapeseed Sclerotinia has a great impact on the quality and yield of rapeseed in my country. It can damage the stems, leaves, flowers, siliques and seeds of rapeseed. The incidence rate of plants infected by Rapeseed Sclerotinia is as high as 10% to 30%. The incidence rate of severely damaged fields can reach more than 80% and lead to a yield reduction of 10% to 70%.

[0003] The Chinese invention patent "CN110663709B A method for preparing a seven-leaf gentian extract and its application" discloses that seven-leaf gentian has a good antibacterial effect on rice blast fungus. However, the current research on seven-leaf gentian is mostly focused on its rhizome, and there is little research on the above-ground parts of seven-leaf gentian such as the pericarp. The present invention aims to explore the antibacterial effect of the pericarp extract of seven-leaf gentian on wheat fusarium head blight, pear black spot, cabbage anthracnose, loofah wilt and rapeseed sclerotinia, in order to discover more medicinal values ​​and potential applications of seven-leaf gentian, which is of great significance for developing it as a natural antibacterial drug. Summary of the invention

[0004] The present invention aims to explore the antibacterial activity of the fruit peel extract of Aesculus quinquefolius against five plant pathogenic fungi, including wheat fusarium head blight, pear black spot, Chinese cabbage anthracnose, loofah wilt and rapeseed sclerotinia. These plant pathogenic fungi not only have a wide range of infection, but also cause great harm. By systematically screening and evaluating the antibacterial effect of the fruit peel extract of Aesculus quinquefolius, its application prospect as a potential antibacterial agent is explored, providing a scientific basis for the development of new natural antibacterial drugs.

[0005] The technical scheme provided by the present invention is the inhibitory effect of a fruit peel extract of Aesculus multiflorus on plant pathogenic fungi. The preparation method of the fruit peel extract of Aesculus multiflorus comprises the following steps: drying the fruit peel of Aesculus multiflorus, grinding it into powder, adding methanol to the powder, dissolving it by ultrasonication, and obtaining a supernatant after centrifugation, drying the supernatant under reduced pressure, and fully dissolving it with distilled water to obtain a solution, extracting the solution with n-butanol, and drying it under reduced pressure again to obtain a concentrated product, and adding methanol to the concentrated product to make a fixed volume to obtain the fruit peel extract of Aesculus multiflorus. The plant pathogenic fungi include wheat fusarium head blight, pear black spot pathogen, cabbage anthracnose pathogen, loofah wilt pathogen and / or rapeseed sclerotinia pathogen.

[0006] Furthermore, the concentration of the extract of the fruit peel of the genus Polygonum multiflorum that inhibits the wheat fusarium rust is 0.56 g / mL to 1.0 g / mL.

[0007] Furthermore, the concentration of the extract of the fruit skin of the genus Aesculus that inhibits the black spot pathogen of pear is 0.84 g / mL to 1.0 g / mL.

[0008] Moreover, the concentration of the extract of the fruit peel of Polygonum multiflorum that inhibits the anthracnose of Chinese cabbage is 0.86 g / mL to 1.0 g / mL.

[0009] Furthermore, the concentration of the extract of Parsley septempunctata peel that inhibits Fusarium wilt pathogen is 0.86 g / mL to 1.0 g / mL.

[0010] Furthermore, the concentration of the extract of the fruit peel of the sclerotinia sclerotiorum that inhibits the rapeseed fungus is 0.022 g / mL to 0.1 g / mL.

[0011] Compared with the prior art, the beneficial effects of this technical solution are:

[0012] 1. The extract of the fruit peel of Aesculus multiflorus provided by the present invention has very ideal inhibitory effects on five plant pathogenic fungi, namely, fusarium wilt of wheat, black spot of pear, anthracnose of cabbage, fusarium wilt of loofah and sclerotinia sclerotiorum of rape. When the concentration is 1 g / mL, the mycelial growth inhibition rates of the four pathogenic fungi, namely, fusarium wilt of wheat, black spot of pear, anthracnose of cabbage and fusarium wilt of loofah, are all above 50%, and the inhibition rate of sclerotinia sclerotiorum of rape is above 50% at 0.1 g / mL.

[0013] 2. The appropriate concentration range of the extract from the fruit peel of Aesculus quinquefolius against five different plant pathogenic fungi was further screened through experiments, providing a solid theoretical basis for the preparation of a new type of plant-based fungicide from the extract from the fruit peel of Aesculus quinquefolius. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The antibacterial effect of different concentrations of Aesculus chinensis peel extract on wheat fusarium head blight. (Note: From left to right, the concentrations of Aesculus chinensis peel extract are mock control, 0.1g / mL, 0.2g / mL, 0.5g / mL, and 1.0g / mL).

[0015] Figure 2 The antibacterial effect of different concentrations of Aesculus chinensis peel extract on pear black spot pathogen (Note: from left to right, the concentrations of Aesculus chinensis peel extract are mock control, 0.1 g / mL, 0.2 g / mL, 0.5 g / mL, and 1.0 g / mL).

[0016] Figure 3 The antibacterial effect of different concentrations of Aesculus chinensis peel extract on Chinese cabbage anthracnose (Note: from left to right, the concentrations of Aesculus chinensis peel extract are mock control, 0.1g / mL, 0.2g / mL, 0.5g / mL, and 1.0g / mL).

[0017] Figure 4 The antibacterial effect of different concentrations of Aesculus chinensis peel extract on Fusarium wilt pathogenic bacteria of luffa (Note: from left to right, the concentrations of Aesculus chinensis peel extract are mock control, 0.1 g / mL, 0.2 g / mL, 0.5 g / mL, and 1.0 g / mL).

[0018] Figure 5 The antibacterial effect of different concentrations of Aesculus chinensis peel extract on rapeseed Sclerotinia sclerotiorum (Note: from left to right, the concentrations of Aesculus chinensis peel extract are mock control, 0.025 g / mL, 0.05 g / mL, 0.1 g / mL, 0.2 g / mL, 0.4 g / mL, 0.8 g / mL, and 1.0 g / mL).

[0019] Figure 6 This is a bar graph showing the antibacterial effect of 0.1 g / mL concentration of the extract from the pericarp of Aesculus chinensis against in vitro inoculated Sclerotinia sclerotiorum of rapeseed.

[0020] Figure 7 The antibacterial effect of the inoculation experiment on detached rapeseed leaves (Note: the concentrations of the extract from the fruit peel of Aesculus quinquefolius from left to right are mock control and 0.1 g / mL). DETAILED DESCRIPTION

[0021] The present invention is described in detail below with reference to the accompanying drawings and embodiments, but the content of the present invention is not limited to the following embodiments.

[0022] 1 Experimental equipment

[0023] 1.1 Experimental Materials

[0024] 1.1.1 Test materials and target pathogen strains

[0025] (1) Seven-leafed Polygonum multiflorum: It is collected from the Wuling Mountain area. The peel extract is dried and ground, evaporated, extracted, etc., and a certain amount of methanol is added to fully dissolve it to prepare an extract.

[0026] (2) Plant pathogenic fungi: wheat fusarium rust, pear black spot, cabbage anthracnose, loofah blight, and rapeseed sclerotinia.

[0027] 1.1.2 Main reagents

[0028] (1) Methanol (anhydrous methanol): Sinopharm Chemical Reagent Co., Ltd.

[0029] (2) n-Butanol: Sinopharm Chemical Reagent Co., Ltd.

[0030] 1.1.3 Preparation of culture medium

[0031] PDA solid culture medium, the specific formula is as follows:

[0032] Table 1 PDA solid culture medium ratio (500mL)

[0033]

[0034] The preparation method is as follows: weigh 100g of potato and cut it into small pieces and put it into a pot, add 700mL of ultrapure water to the pot, add it to boil over low heat, keep boiling for 20min, and stir it continuously with a glass rod to prevent the bottom from sticking. Filter the cooked potato turbid liquid through two layers of filter gauze into a measuring cup, and discard the filter residue. Weigh 10g of glucose and 9g of agar into a measuring cup, place the measuring cup on an ultrasonic machine to fully dissolve and mix, and then add ultrapure water to make the volume 500mL. The filtrate after volume is drained into a 1500mL conical flask with a glass rod, sealed with a sealing film, marked, and placed in a high-temperature and high-pressure sterilizer, sterilized at 115℃ for 20min, and poured onto a clean bench after sterilization. After blowing for 3h, seal and store in a 4℃ refrigerator for later use.

[0035] 1.2 Main instruments

[0036] (1) Electronic balance (ALC-2100.2, Shenzhen Taili Instrument Co., Ltd.);

[0037] (2) Ultrapure water device (UPW-20NT, Beijing Liyuan Electronic Instrument Co., Ltd.);

[0038] (3) High temperature and high pressure autoclave (HVA-110, Hirayama, Japan);

[0039] (4) Clean bench (SWC-JA, Shanghai Xinmiao Medical Device Manufacturing Co., Ltd.);

[0040] (5) Constant temperature incubator (SPX-250BS-Ⅱ Shanghai Xinmiao Medical Equipment Manufacturing Co., Ltd.);

[0041] (6) Refrigerated centrifuge Eppendorf Cenrifuge 5417R, 5810R (Eppendorf, Germany).

[0042] 2 Experimental methods

[0043] 2.1 Preparation of the extract from the fruit peel of Aesculus quinquefolius

[0044] (1) Take the dried fruit peel of the seven-leaf lily flower and add it to a mortar, pour an appropriate amount of liquid nitrogen to quickly freeze the fruit peel, and grind it into powder with a grinding rod evenly and forcefully in circles, pass it through a 40-mesh sieve, and weigh it to 15 g;

[0045] (2) Divide the above powder into two halves and pour into a 1500 mL conical flask. Add 750 mL of methanol and ultrasonically dissolve for 1 h. Centrifuge at 4000 rpm at 4°C for 20 min, then take the supernatant and pour into a new conical flask.

[0046] (3) Pour an appropriate amount of the obtained supernatant into a round-bottom flask, use a rotary evaporator at 58°C to dry the solution under reduced pressure, repeat this operation several times until all the supernatant is dried, and then wash the round-bottom flask thoroughly with distilled water to fully dissolve;

[0047] (4) extracting the above solution with n-butanol 2-3 times;

[0048] (5) Pour an appropriate amount of the active ingredients obtained after extraction into a new round-bottom flask and dry them again under reduced pressure at 70°C using a rotary evaporator until all the active ingredients are dried;

[0049] (6) The concentrated product after drying was diluted with methanol to 15 mL, and the concentration of the extract of Psoralea corylifolia was 1 g / mL. The solution was stored in a refrigerator at 4°C for later use.

[0050] 2.2 Activation of plant pathogenic fungi

[0051] On the clean bench, wheat fusarium rust, pear black spot pathogen, cabbage anthracnose pathogen, loofah blight pathogen and rapeseed sclerotinia pathogen were activated, and the bacterial blocks were picked up with high-temperature sterilized tweezers and placed in the center of a new PDA culture medium. Each strain was treated with 3 replicates, and a total of 15 PDA solid culture media were inoculated. The culture media were sealed with sealing film, marked, and finally placed in a constant temperature incubator at 28°C. The pear black spot pathogen was cultured under 8 hours of light and 16 hours of darkness every day.

[0052] 2.3 Configuration of plant extract concentration

[0053] Take out the prepared Aesculus quinquefolius peel extract stock solution, mix 1 g / mL of the extract with sterilized ultrapure water in proportion to prepare 1 mL of dilution solution, including four concentration gradients of 0.1 g / mL, 0.2 g / mL, 0.5 g / mL, and 1.0 g / mL, and set up a mock control.

[0054] 2.4 Preparation of culture medium

[0055] Prepare 500mL PDA culture medium, divide it into 5 parts before sterilization, and sterilize it in a high-temperature and high-pressure autoclave. Take out the sterilized culture medium, wait for it to cool to 60-65°C, add the prepared extracts of various concentrations to the culture medium and shake to mix, and use PDA culture medium mixed with an equal volume of sterilized ultrapure water as a mock control. Finally, pour it into a flat plate and blow it for 3 hours before storing it for use.

[0056] 2.5 Identification of mycelial growth inhibitory activity

[0057] Adopt the virus culture method. Use the tip of a 100μL pipette to press and rotate the edge of the cultured colony to obtain a bacterial cake with complete and consistent growth conditions. Use sterilized tweezers to pick up the bacterial cake and place it in the center of the above-mentioned virus-containing culture medium. Each group is treated with 5 replicates. The inoculated culture medium is placed in a 28℃ constant temperature incubator for culture, where the pear black spot pathogen is maintained under 8h light and 16h dark conditions. Until the culture medium colony of the mock control group is full, observe and measure the plaque diameter of each treatment using the cross method, and calculate the mycelium growth inhibition rate. Relative inhibition rate (%) = (control group colony diameter - treatment group colony diameter) / control group colony diameter × 100%.

[0058] 2.6 Toxicity determination of extracts from the fruit peel of Aesculus quinquefolius

[0059] According to the above data, the concentration of the extract from the fruit peel of Aesculus septififlorus was used as the horizontal axis and the mycelium growth inhibition rate was used as the vertical axis. SPSS22.0 was used to simulate the toxicity regression equation and calculate the EC 50 value and calculate the correlation coefficient R 2 .

[0060] 3 Experimental results

[0061] 3.1 Antibacterial effect of the extract from the fruit peel of Aesculus quinquefolius against wheat fusarium

[0062] From Table 2 and Figure 1 It can be seen that compared with the control (mock), the extract of the fruit peel of Aesculus quinquefolius has a significant inhibitory effect on the mycelium of wheat fusarium (P < 0.05), and the antibacterial effect of the extract is positively correlated with the concentration used. At 0.1g / mL, 0.2g / mL, 0.5g / mL, and 1.0g / mL, the mycelial growth inhibition rates of wheat fusarium were 9.8%, 18.63%, 45.43%, and 88.42%, respectively. Overall, the extract of Aesculus quinquefolius has a strong inhibitory effect, but there is no significant difference in the mycelial inhibition rate at concentrations of 0.1g / mL and 0.2g / mL. As the concentration increases, the difference becomes increasingly obvious.

[0063] Table 2 Antibacterial effect of different concentrations of Aesculus chinensis peel extract on wheat fusarium sphaerocephala

[0064]

[0065] Note: The lowercase letters after the data in the same column indicate whether the difference between different concentrations is significant (P < 0.05). The same applies to the following table.

[0066] 3.2 Antibacterial effect of the extract from the fruit peel of Aesculus chinensis against pear black spot pathogen

[0067] From Table 3 and Figure 2It can be seen that compared with the mock control group, the extract of Aesculus quinquefolius had a certain degree of inhibitory effect at different concentrations (P < 0.05). However, when the concentration was low, the inhibitory effect on mycelial growth was not much different. As the concentration increased, the inhibitory effect was proportional to the concentration used, and the mycelial growth inhibition rate increased significantly. At the four concentrations of Aesculus quinquefolius extract, the mycelial growth inhibition rates of pear black spot pathogen were 12.03%, 15.62%, 34.17% and 56.61%, respectively. In summary, the extract of Aesculus quinquefolius can have a significant inhibitory effect on the mycelial growth of pear black spot pathogen.

[0068] Table 3 Antibacterial effect of different concentrations of Aesculus chinensis peel extract on pear black spot pathogen

[0069]

[0070] 3.3 Antibacterial effect of the extract from the fruit peel of Aesculus chinensis against the anthracnose pathogen of Chinese cabbage

[0071] From Table 4 and Figure 3 It can be seen that compared with the mock control, the extract of seven-leaf clematis has a significant effect on the mycelial growth of Chinese cabbage anthracnose at a certain concentration (P < 0.05), and the inhibitory effect is positively correlated with the concentration used. At 0.1g / mL, 0.2g / mL, 0.5g / mL, and 1.0g / mL, the mycelial growth inhibition rates of Chinese cabbage anthracnose were 3.61%, 26.71%, 30.38%, and 51.47%, respectively. From the difference analysis of colony diameter and mycelial growth inhibition rate, the inhibition rate was only 3.61% under the action of 0.1g / mL of seven-leaf clematis extract, indicating that the antibacterial effect was not significant. The inhibitory effect between the two concentrations of 0.2g / mL and 0.5g / mL was not significant, and it had a very significant inhibitory effect at 1.0g / mL. In general, the extract of seven-leaf clematis has no inhibitory effect at a lower concentration, and has a very significant inhibitory effect at a higher concentration.

[0072] Table 4 Antibacterial effect of different concentrations of Aesculus chinensis peel extract on Chinese cabbage anthracnose

[0073]

[0074] 3.4 Antibacterial effect of the extract from the fruit peel of Aesculus chinensis against Fusarium wilt pathogen

[0075] From Table 5 and Figure 4It can be seen that compared with the mock control, the extract of Aesculus quinquefolius has an inhibitory effect on the hyphae of the fungus of the fungus of the loofah blight (P < 0.05), and the inhibitory effect is positively correlated with the concentration used. When the concentration of the extract of Aesculus quinquefolius is 0.1g / mL, 0.2g / mL, 0.5g / mL, and 1.0g / mL, the inhibition rates of mycelial growth are 6.21%, 14.05%, 36.93%, and 53.59%, respectively. At lower concentrations, i.e., 0.1g / mL and 0.2g / mL, there is no significant difference in the mycelial growth inhibition rate, which is only 6.21% and 14.05%. As the concentration increases, the difference between the concentrations increases, and the inhibitory effect is more obvious. This shows that the extract of Aesculus quinquefolius still has a significant inhibitory effect on the fungus of the fungus of the loofah blight at high concentrations.

[0076] Table 5 Antibacterial effect of different concentrations of Aesculus septoria peel extract on Fusarium wilt pathogen

[0077]

[0078]

[0079] 3.5 Antibacterial effect of the extract of Pseudomonas aeruginosa peel on Sclerotinia sclerotiorum of rapeseed

[0080] Take out the prepared extract of the fruit peel of seven-leaf lily, dilute the extract and prepare it into different concentrations of 0.025g / mL, 0.05g / mL, 0.1g / mL, 0.2g / mL, 0.4g / mL, 0.8g / mL, and 1.0g / mL, and then mix methanol and sterile water in proportion to prepare a dilution as a mock control. Take 100μL of plant extract and spread it evenly on the culture medium. Inoculate the bacterial cake in the middle of the culture medium, and inoculate 5 plates at each concentration. Place the plate in a constant temperature incubator at 25℃ for 3 days and then take it out, observe and measure the plaque diameter by the cross method, and calculate the mycelium growth inhibition rate by the formula.

[0081] 3.5.1 Antibacterial activity of different concentrations of extracts from the fruit peel of Aesculus quinquefolius

[0082] like Figure 5 As shown in Table 6, all seven concentrations have a certain inhibitory effect on rapeseed sclerotinia. The higher the concentration, the better the antibacterial effect. Among them, the extract with a concentration of 1.0g / mL has the best effect, and the mycelial growth inhibition rate reaches 100%. The extract with a concentration of 0.025g / mL has an antibacterial effect, and the mycelial growth inhibition rate also reaches 57.11%. Through the significance test analysis, it can be obtained that the antibacterial rates of the extracts of the seven concentrations are significantly different from those of the control group (P<0.01), and all have good antibacterial effects.

[0083] Table 6 Antibacterial effect of different concentrations of Aesculus septoria peel extract on Sclerotinia sclerotiorum

[0084]

[0085] 3.5.2 Inoculation of detached rapeseed leaves

[0086] Cut rapeseed leaves with good growth and uniform size and place them in a tray. Punch 1-2 holes symmetrically on both sides of the leaves, and inoculate the activated bacteria cakes at the punched parts. Seal the tray with plastic wrap and puncture a small number of holes to keep it breathable. 24 hours after inoculation, spray the experimental groups with 0.1g / mL of the fruit peel extract of Aesculus multiflorus, and set up a mock control; observe, measure and record the size of rapeseed lesions 2 days after inoculation, and use the formula to calculate the relative control effect.

[0087] like Figure 6 and Figure 7 As shown in the figure, the lesion area of ​​the rapeseed leaf after inoculation with pathogens in the control treatment was 11.80 cm 2 Compared with the control, the lesion area of ​​the experimental group sprayed with 0.1 g / mL of the fruit peel extract of Aesculus chinensis was 4.62 cm 2 , the lesion area was smaller, and the control effect was 60.59%, indicating that 0.1 g / mL of the extract from the pericarp of Aesculus septoria could effectively inhibit the infection and growth of the mycelium of Sclerotinia sclerotiorum on rapeseed leaves, and the difference among the treatments was extremely significant (P<0.001).

[0088] 3.6 Results of toxicity test of the extract of Psoralea corylifolia peel

[0089] EC 50 The inhibition median concentration is a specific concentration when the antibacterial effect of the substance reaches 50%, and its size is related to the degree of inhibition of the plant extract. As shown in Table 7, the inhibition median concentrations of the extracts from the fruit peel of Aesculus chinensis against the five pathogenic fungi are 0.022g / mL, 0.560g / mL, 0.841g / mL, 0.859g / mL, and 0.860g / mL, respectively. 50 The order is rapeseed sclerotinia < wheat fusarium < pear black spot < Chinese cabbage anthracnose < loofah wilt, indicating that seven-leafed gentian is the most sensitive to rapeseed sclerotinia and has the best effect. But in general, seven-leafed gentian has a good inhibitory effect on the above five pathogenic fungi.

[0090] Table 7 Regression analysis of the extract of Aesculus chinensis peel on five pathogenic fungi

[0091]

[0092] Experiments have shown that the Aesculus quinquefolius peel extract prepared by the present invention has an inhibitory effect on the selected five plant pathogenic fungi. The Aesculus quinquefolius peel extract has an inhibition rate of more than 50% on the mycelial growth of four pathogenic fungi, namely, wheat fusarium head blight, pear black spot fungus, vegetable anthracnose fungus, and loofah wilt fungus, when the concentration is 0.5-0.8 g / mL. The inhibition rate of the Aesculus quinquefolius peel extract on the rapeseed sclerotinia fungus is more than 50% at 0.022 g / mL, indicating that the Aesculus quinquefolius peel extract can achieve an ideal inhibitory effect on the above five plant fungi at a suitable concentration, which can provide new ideas for the development of biological pesticides for the prevention and treatment of plant diseases, and provide a solid theoretical basis for Aesculus quinquefolius as a new botanical fungicide.

Claims

1. The inhibitory effect of the fruit peel extract of Aesculus multiflorus on plant pathogenic fungi. The preparation method of the fruit peel extract of Aesculus multiflorus is as follows: drying the fruit peel of Aesculus multiflorus, grinding it into powder, adding methanol to the powder and dissolving it by ultrasonication, and obtaining a supernatant after centrifugation, drying the supernatant under reduced pressure, and fully dissolving it with distilled water to obtain a solution, extracting the solution with n-butanol, and drying it under reduced pressure again to obtain a concentrated product, and adding methanol to the concentrated product to obtain a fruit peel extract of Aesculus multiflorus, characterized in that: The plant pathogenic fungi include wheat fusarium wilt, pear black spot fungus, cabbage anthracnose fungus, loofah wilt fungus and / or rapeseed sclerotinia fungus.

2. The inhibitory effect of the extract of Parsley chinensis peel on plant pathogenic fungi according to claim 1 is characterized in that: The concentration of the extract of the fruit peel of Aesculus quinquefolius that inhibits wheat fusarium rust is 0.56 g / mL to 1.0 g / mL.

3. The inhibitory effect of the extract of Parsley fruit peel on plant pathogenic fungi according to claim 1 is characterized in that: The concentration of the extract of the fruit peel of Aesculus quinquefolius that inhibits the black spot pathogen of pear is 0.84 g / mL to 1.0 g / mL.

4. The inhibitory effect of the extract of Parsley fruit peel on plant pathogenic fungi according to claim 1, characterized in that: The concentration of the extract from the fruit peel of Polygonum multiflorum that inhibits the anthracnose of Chinese cabbage is 0.86 g / mL to 1.0 g / mL.

5. The inhibitory effect of the extract of Parsley fruit peel on plant pathogenic fungi according to claim 1, characterized in that: The concentration of the extract of the fruit peel of Aesculus quinquefolius that inhibits the wilt pathogen of loofah is 0.86 g / mL to 1.0 g / mL.

6. The inhibitory effect of the extract of Parsley fruit peel on plant pathogenic fungi according to claim 1, characterized in that: The concentration of the extract of the fruit peel of Aesculus multiflorus that inhibits the rapeseed sclerotinia pathogen is 0.022 g / mL to 0.1 g / mL.

Images