Lactobacillus plantarum dx7, method for producing indolelactic acid and use thereof

By adding deep-striped walnut inner seed coat polyphenols and L-tryptophan to the culture medium of Lactobacillus plantarum DX7, its tryptophan metabolic pathway is activated, solving the problem of low indole-lactic acid synthesis efficiency of Lactobacillus plantarum under conventional conditions. This achieves efficient and safe indole-lactic acid production, which can be applied to alleviate inflammatory bowel disease.

CN120796139BActive Publication Date: 2026-05-29KUNMING UNIV OF SCI & TECH
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Patent Information

Application Number
CN202511060340.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-07-30
Publication Date
2026-05-29
Estimated Expiration
2045-07-30

AI Technical Summary

Technical Problem

In existing technologies, Lactobacillus plantarum has low efficiency in synthesizing indole-lactic acid under normal conditions, which is difficult to meet the needs of industrial production. Furthermore, genetic engineering modification strategies are costly and pose biosafety risks.

Method used

The tryptophan metabolic pathway of Lactobacillus plantarum DX7 was activated by polyphenols from the inner seed coat of deep-striped walnut. By adding polyphenols from the inner seed coat of deep-striped walnut and L-tryptophan to the culture medium, the aromatic amino acid aminotransferase and lactate dehydrogenase of Lactobacillus plantarum DX7 were activated, thus achieving efficient biosynthesis of indolelacic acid.

Benefits of technology

It increases the yield of indolelactic acid, reduces production costs, meets the industrial demand for indolelactic acid in the food sector, and has biosafety, and can alleviate inflammatory bowel disease.

✦ Generated by Eureka AI based on patent content.

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Abstract

The application provides a lactobacillus plantarum DX7 and a method and application thereof for producing indole lactic acid, relates to the field of food microbial technology, and the lactobacillus plantarum DX7 has been preserved in the Guangdong Microbial Culture Collection Center on June 12, 2025, and the preservation number is GDMCC NO.66498. The lactobacillus plantarum DX7 in the application is significantly activated under the stimulation of deep-line walnut inner seed coat polyphenol, and can efficiently convert tryptophan into ILA. Experimental results show that the ILA yield of the strain reaches 217.56 mu g / mL. Moreover, the strain and deep-line walnut inner seed coat polyphenol can significantly relieve the colon inflammation symptoms of a DSS-induced colon inflammation mouse by gavage. The application not only provides a new strategy for efficient biological preparation of the functional component ILA, but also provides a basis and potential solution for dietary intervention of inflammatory bowel disease.
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Citation Information

Patent Citations

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