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Active part of Sambucus williamsii Hance for reducing risk of bone-related diseases of menopausal women and application thereof

A technology for bone-related diseases and active parts, applied in bone diseases, organic active ingredients, medical preparations containing active ingredients, etc., can solve problems such as shortage, and the mechanism of action of elderberry's anti-osteoporosis active parts is not yet clear, and achieve Effects of improving bone density, improving bone loss, and increasing uterine weight

Active Publication Date: 2010-07-14
THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the above-mentioned ingredients lacks the experimental evidence of animal pharmacodynamics in vivo, and the anti-osteoporosis active site of elderberry and its mechanism of action are not yet clear.

Method used

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  • Active part of Sambucus williamsii Hance for reducing risk of bone-related diseases of menopausal women and application thereof
  • Active part of Sambucus williamsii Hance for reducing risk of bone-related diseases of menopausal women and application thereof
  • Active part of Sambucus williamsii Hance for reducing risk of bone-related diseases of menopausal women and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the preparation method of elderberry active site

[0047] Take 50 kilograms of dry stems and branches of elderberry, after proper pulverization, heat and reflux extraction with 10 times the amount of 60% ethanol for 3 times, each time for 2 hours. The extracts were combined, and the solvent was evaporated under reduced pressure to obtain 2160 grams of the total extract of Elderberry. Get 1250 grams of elderberry total extract subsequently, dissolve with suitable amount of water, carry out macroporous resin open column chromatography, use the water of 5 times column bed volumes, 30%, 50%, 95% ethanol-water solution gradient elution successively, Collect each part eluent, reclaim solvent under reduced pressure respectively, obtain water elution part 712.5 grams, 30% ethanol elution part 160 grams, 50% ethanol elution part 125 grams and 95% ethanol elution part 111 grams, wherein 50 The fraction eluted with % ethanol is the active site of elderberry (for act...

Embodiment 2

[0048] Example 2: Isolation and Identification of Main Components in Elderberry Active Parts

[0049] Elderberry 50% ethanol elution part prepared in embodiment 1 has determined its fingerprint by HPLC analysis liquid phase, see figure 1 . Under the guidance of fingerprints, through ODS column chromatography, HW 40 column chromatography, RP-HPLC preparative liquid phase separation means, and through HPLC, MS, NMR and other analysis and identification methods, 7 compounds were identified, vanillic acid (1), coniferyl alcohol (2), L-threo-guaiacol-β-O-4'-coniferyl alcohol ether (3), D-erythro-1-(4-hydroxy-3-methoxyphenyl)- 2-[-4-(3-Hydroxypropyl)-2-hydroxyphenoxy]-1,3-propanediol (4), D-erythroguaiacol-β-O-4′- Coniferyl alcohol ether (5), dehydrodisconiferyl alcohol (6), dihydrodehydrodisconiferyl alcohol (7).

[0050] The compound structural formula obtained by separation is shown in figure 2 . Under the same chromatographic conditions as the Elderberry 50% ethanol elutio...

Embodiment 3

[0096] Example 3: The establishment of fingerprints and the identification of each compound

[0097] 3.1 Establishment of fingerprint

[0098] Take 40 mg of the 50% ethanol-eluted part of the above elderberry, dissolve it in 2 mL of 60% methanol-water, pass it through an ODS solid-phase extraction column, elute with 10 mL of methanol, evaporate the eluate to dryness, dissolve it in 2 mL of methanol, and filter through 0.45 μm film, to obtain the test solution of the active part of elderberry.

[0099] Precisely get 10 μL of the above-mentioned test solution, refer to the high-performance liquid phase analysis method specified in the appendix of the National Pharmacopoeia, inject high-performance liquid chromatography for chromatographic analysis, and record the chromatogram (see figure 1 ). The liquid chromatographic conditions are: using octadecylsiloxane bonded silica gel as the stationary phase; using methanol-water solution containing 0.1% acetic acid as the mobile phase...

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Abstract

The invention discloses an active part of Sambucus williamsii Hance with the effect of reducing bone mass loss after menopause. The active part has definite chemical components and mainly comprises seven compounds: vanillic acid, coniferol, levo erythro form guaiacyl glycerol-beta-O-4'-coniferyl alcohol ether, dextro threo form-1-(4-hydroxy-3-methoxyphenyl)-2-[-4-(3-hydroxypropyl)-2-hydroxyphenoxy]-1,3-propanediol, dextro threo form guaiacyl glycerol-beta-O-4'-coniferyl alcohol ether, coniferyl alcohol dehydrogenase and dihydro-coniferyl alcohol dehydrogenase. The active part can remarkably reduce the bone loss of ovariectomized mice, increase bone density of thighbone and shinbone, increase bone volume, does not increase the weight of uterus, and is a component for safely and effectivelyrelieving and improving various symptoms of the bone-related diseases of the menopausal women. The invention has the advantages of providing the active part of the Sambucus williamsii Hance which canbe used for preparing a medicament, food or a food additive for preventing and treating the bone-related diseases after menopause.

Description

technical field [0001] The invention relates to the protective effect of the active part of elderberry on postmenopausal bone, specifically the application of the active part of elderberry and the active ingredient contained in the preparation of medicine or health care product for preventing or treating postmenopausal bone-related diseases. Background technique [0002] In the 20th century, with the improvement of medical level and the extension of human life expectancy, the survival time of postmenopausal women has been significantly extended. Therefore, the incidence of diseases such as climacteric syndrome, obesity, osteoporosis, urogenital atrophy, diabetes, cardiovascular disease and senile dementia caused by menopause has also increased significantly. These diseases not only affect the quality of life of patients, but also bring a heavy burden to society and families. [0003] Within 1 to 10 years after menopause, women will experience a rapid loss of calcium in thei...

Claims

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Application Information

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IPC IPC(8): A61K31/343A61K31/192A61K31/09A61K31/085A61K36/35A61P19/00A23L1/29A23L1/30A61K135/00A23L33/105
Inventor 黄文秀姚新生肖辉辉戴毅
Owner THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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