Method for detecting types and contents of soil phenolic acids in eucalyptus forest land

Abstract

The invention relates to a method for detecting types and contents of soil phenolic acids in a eucalyptus forest land. The method comprises the following steps: sampling a soil sample in a forest land and bringing back to a laboratory for later use; preparing a phenolic acid mixed standard solution, injecting into efficient high performance liquid chromatography, determining proper wavelength and chromatographic conditions, carrying out linear regression according to phenolic acid concentration and peak area of a map to draw a standard curve; preparing a sample solution for test, and obtaining a sample solution by adding a NaOH solution, centrifugalizing, acidifying by virtue of hydrochloric acid, removing humic acid, filtering and the like; and feeding the sample by using an automatic sampler, measuring according to the chromatographic condition, recording the peak area, and calculating the types and contents of soil phenolic acids according to the standard curve of the phenolic acid mixed solution. The detection method provided by the invention is simple to operate, high in detection speed, high in sensitivity, strong in accuracy, good in repeatability and suitable for detecting the types and contents of p-hydroxybenzoic acid, vanillic acid, ferulic acid, cumaric acid, benzoic acid, salicylic acid and cinnamic acid in forest land soil.

Description

technical field [0001] The invention relates to a method for detecting phenolic acid compounds, in particular to a method for detecting the type and content of phenolic acid in eucalyptus woodland soil. Background technique [0002] In the life activities of plants, phenolic acids are one of the important secondary metabolites. Its biosynthesis is mainly through the shikimic acid pathway in the plant physiological process, and enters the soil through the secretion of plant roots, the leaching of the aboveground parts, the decomposition of litter and organic matter, and the activities of microorganisms, etc., affecting the nutrients in the soil. The effective form of substances and the distribution of microbial populations. Some studies believe that the accumulation of phenolic acids in the soil is one of the important factors that hinder the continuous cropping of crops. With the increasing productivity decline of multi-generation continuous planting in artificial pure fore...

AI Technical Summary

Problems solved by technology

Some studies believe that the accumulation of phenolic acids in the soil is one of the important factors that hinder the continuous cropping of crops. With the increasing productivity decline of multi-generation continuous planting in artificial pure forests in my country in recent years, phenolic acids in forest soil It has also begun to attract the general attention of many scholars, who believe that phenolic substances may accumulate in the soil, thereby poisoning the soil, and become one of the important reasons for the continuous planting of plantations to reduce production. The report affe

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