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Compound probiotic fermentation product having effect of protecting skins, and preparation and applications thereof

A technology of probiotics and fermented liquid, applied in skin care preparations, medical preparations containing active ingredients, fermentation, etc., can solve the problem of lack, lack of stable fermentation process, high active metabolite enrichment process, and lack of active metabolites Skin care function and other issues, to achieve the effect of repairing the skin barrier, improving skin care efficacy, and maintaining the balance of flora

Active Publication Date: 2021-06-18
青岛元达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the prior art lacks research on the stable fermentation process of probiotic metabolites, the enrichment process of highly active metabolites, and the skin care function; specifically, (1) the lack of fermentation media optimized for skin care functions with high yields of probiotic active metabolites , time, temperature and other fermentation technologies, thereby improving the yield and stability of beneficial metabolites; (2) lack of fermentation technology using compound lactic acid bacteria fermentation technology to produce highly active and rich types of beneficial substances; (3) lack of active metabolism Research on skin care function

Method used

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  • Compound probiotic fermentation product having effect of protecting skins, and preparation and applications thereof
  • Compound probiotic fermentation product having effect of protecting skins, and preparation and applications thereof
  • Compound probiotic fermentation product having effect of protecting skins, and preparation and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: A post-bilantrium Bilantera with skin care function and its preparation

[0032] (1) CGMCC No. 11932, Cheese Lactobacillus CGMCN NO.18638, Milk Bifidobacterium CGMCC NO.14536, Animal Bifidobacterium CGMCS NO.14536, Rat Plumi CGMCC NO.16002 In the MRS liquid medium, at 37 ° C constant temperature culture medium for 18 h, biochemical 2 times, so that the number of live bacteria after the activation of each strain is 50-20 billion CFU / mL.

[0033] (2) Activated plant Lactobacillus CGMCC No. 11932, Cheese Lactobacillus CGMCS NO.18638, Milky Bifidobacterium CGMCC NO.14536, Animal Bifidobacter cGMCC NO.16070 and Rasmurogonia CGMCCN16002 The proportion of strains is 1: 1: 2: 2: 0.5 in ratio of 1: 1: 2: 2: 0.5.

[0034](3) The above-mentioned probiotic composition is inoculated in a fermentation medium in a fermentation medium according to 2% inoculation. The formulation of fermentation medium is: sucrose 50g, algae powder 8 g, degreasing soy powder 20g, degreased milk...

Embodiment 2

[0039] Example 2: Rear Birage Bilantera Skin Care Function

[0040] Through the clinical efficacy test of the post-bubble Bilantera, the changes in the indicators of the skin before and after application were studied to verify the effect of wrinkling and repair the skin barrier. The reference standard is: "Cosmetics Moisturizing Efficiency Evaluation Guide" QB / T4256-2011, "Cosmetics Impact Test Method" T / ENCA003-2018 and Mechanism Internal Test Standard.

[0041] The test method is as follows:

[0042] (1) Test Sample: Example 1 Rear Bilantera;

[0043] Control samples: 1% thickener, 0.5% preservative, 3% wetting agent, 2% stabilizer, and the rest is deionized water.

[0044] (2) Test instrument

[0045] Facial image analysis of Visia image analysis software;

[0046] Skin elastic test probe Cutometer MPA580 (Courage & Khazaka, Germany);

[0047] Skin Black and Red Pinchin Test Probes Mexametermx18 (Courage & Khazaka, Germany);

[0048] Skin moisture content probe CorneometerC...

Embodiment 3

[0083] Example 3: Content of active substances in probiotics fermentation

[0084] The post-birth fermentation liquid prepared in Example 1 was a test sample, based on the UPLC-Q-TRAP-MS targeted metabolic analysis method, the active material and content were analyzed, and the specific is as follows:

[0085] (1) Pre-sample treatment

[0086] 0.1 g of the sample was added to a 2 ml homogenating tube containing 1.0 mm zirconia bead, and 1 ml of ultrapure water was mixed and mixed using Omni Bead Ruptor 12 (Omni International, USA). Extra slurry under 3 m / s conditions. Quality 30s was repeated 2 times; all transferred to 7 ml of centrifuge tube, 30 μl of 50 μg / ml of bile acid mixed IS solution and 2 ml of acetonitrile were mixed, and 13400 × g centrifuge for 10 min. Take the 0.22 μm filter film, 50 μl was transferred to a 2 ml liquid sample bottle equipped with 450 μl of methanol, and it was configured. (2) UPLC-Q-TRAP-MS detection conditions

[0087] Liquid phase conditions: Th...

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Abstract

The invention belongs to the field of microbial fermentation, and especially relates to a fermentation metabolite having an effect of protecting skins, and a preparation method and applications thereof. The product is prepared by taking lactobacillus plantarum, lactobacillus casei, lactobacillus lactentis, bifidobacterium animalis and lactobacillus rhamnosus as fermentation strains and taking sucrose, seaweed meal, soybean meal and milk powder as fermentation raw materials through mixing and fermentation. The product contains specific fermentation metabolites, such as malonylgenistin, genistein, hyaluronic acid, irone, Preaustinoid A and daidzein, which are helpful for the enhancement of a skin protection effect; and tartaric acid, malic acid, succinic acid, salicylic acid, vanillic acid, phenylpropionic acid and other organic acid generated by fermentation can control pathogenic bacteria and spoilage microbial flora, so that skin flora balance can be maintained, and a natural antiseptic effect can be achieved. Efficacy tests show that the product has the efficacy of removing wrinkles, increasing skin elasticity, resisting inflammation and repairing skin barriers, and is significant in effect.

Description

Technical field: [0001] The present invention relates to a probiotic fermentation product, and more particularly to a fermentation metabolite having skin care functions and a preparation method thereof, which belongs to the microbial fermentation. Background technique: [0002] Lactic acid bacteria and its fermentation products play an important role in protecting human healthy life, is a people's livelihood industry carrying human nutrition and health. A large number of studies have shown that probiotics have related effects of probiotics and their activity, especially probiotics metabolites, bacterial lysis products and bacteria themselves may be directly pushing for health efficacy, these ingredients are named after the international probiotics . Promoted metabolites produced by lactic acid bacteria, extracellular polysaccharide, cell wall peptide, short-chain fatty acid, lactic acid bacteria, antibacterial organic acid, etc., in the regulating body immunity, anti-tumor, antib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12N1/20A61K8/99A61Q17/00A61Q19/00A61Q19/08C12R1/25C12R1/245C12R1/225C12R1/01
CPCC12P1/04C12N1/20A61K8/99A61Q19/00A61Q19/08A61Q17/005A61K2800/5922A61K2800/85A61K2800/524Y02A40/90
Inventor 彭传涛王习振
Owner 青岛元达生物科技有限公司
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