Amorpha fruticosa aerial seeding seed pelleting treatment method and equipment for improving germination rate
By granulating Amorpha fruticosa seeds and using adhesives and carriers to form a protective layer, the problem of difficult germination of Amorpha fruticosa seeds during aerial sowing was solved, resulting in a high germination rate and survival rate, and adaptability to various environmental conditions.
Patent Information
- Application Number
- CN202511385345.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-26
- Publication Date
- 2025-12-19
AI Technical Summary
The seeds of Amorpha fruticosa are hard and brittle with a thin shell, making them difficult to germinate effectively during aerial sowing. Traditional broadcast sowing methods make it difficult to control germination time and amount, resulting in difficulties in management and achieving ideal cultivation results.
The pelleting process involves cleaning, disinfection, soaking, preparing adhesives and endophytic bacterial solutions, and using maifanite and bentonite as carriers to form spherical particles. This enhances the physical properties of the seeds, forms a protective layer, and helps them adapt to harsh environments.
It significantly improves the germination and survival rate of Amorpha fruticosa seeds, reduces drift and landing displacement, avoids damage from birds and rodents, enhances moisture retention, adapts to arid and saline-alkali environments, and achieves uniform sowing and precise control.
Smart Images
Figure CN121153410A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of forest tree seed treatment, and more specifically, to a method and equipment for pelleting Amorpha fruticosa aerial seeds to improve germination rate. Background Technology
[0002] Amorpha fruticosa is an important ecological afforestation tree species, widely used in projects such as returning farmland to forest and protecting natural forests, and is especially suitable for large-scale aerial seeding. However, during aerial seeding, due to certain characteristics of Amorpha fruticosa seeds and the complexity of the aerial seeding environment, many problems are often encountered.
[0003] However, the seeds of the Amorpha fruticosa are hard and brittle, with a very thin shell, and must not be waterlogged before germination. As a material for seed propagation, it not only boasts rapid propagation, strong adaptability, and high economic value, but also exhibits cold resistance, drought resistance, and salt tolerance. It has excellent wood quality, grows rapidly, and is suitable as a superior tree species for courtyards and streets, as well as for afforestation of barren hills. However, because its seeds are hard and oily, they are difficult to germinate (direct sowing almost never results in germination). Therefore, seed propagation is commonly carried out through sowing and cuttings. Mechanical sowing can damage the endosperm layer, affecting seed germination. The traditional method of directly scattering seeds on the ground and allowing them to germinate freely is difficult to control in terms of germination time and quantity, leading to difficulties in management and ultimately failing to achieve the desired results.
[0004] To address the aforementioned problems, a technical solution is provided. Summary of the Invention
[0005] In order to overcome the above-mentioned defects of the prior art, the embodiments of the present invention provide a method and equipment for pelleting Amorpha fruticosa aerial seeds to improve germination rate, so as to solve the problems mentioned in the background art.
[0006] To achieve the above objectives, the present invention provides the following technical solution:
[0007] A method for pelleting Amorpha fruticosa aerial seeds to improve germination rate includes the following steps:
[0008] Step S1: Clean, disinfect, and soak the seeds of Amorpha fruticosa. After soaking, rinse with clean water and then air dry.
[0009] Step S2: Collect fresh seeds of Koelreuteria paniculata to prepare endophytic bacterial solution, prepare adhesive, and stir the adhesive evenly and completely to dissolve it;
[0010] Step S3: Add the Amorpha fruticosa seeds and adhesive to the mixer and mix evenly. Then add the endophytic bacterial solution and continue mixing. Next, use maifanite and bentonite as carriers to quickly coat the seeds evenly and obtain the mixture.
[0011] Step S4: Mix the mixture and then granulate it.
[0012] Step S5: The granulated material is dried and screened, and finally bagged and stored.
[0013] In a preferred embodiment, in step S1, obvious impurities such as stones and leaves are first removed manually from the Amorpha fruticosa seeds. Then, the seeds are placed in a container and soaked in water for 2-3 hours to soften the attached substances. Next, the seeds are gently rubbed by hand to remove the pulp and impurities. Different sieves are used to filter the seeds and separate them from the impurities. After that, the seeds are rinsed with water until the water becomes clear. Finally, the cleaned seeds are placed on a towel or paper towel and placed in a ventilated place to dry, avoiding direct sunlight.
[0014] In a preferred embodiment, in step S1, the washed seeds are soaked in a 1% potassium permanganate solution for 10-15 minutes to kill some of the pathogens, pests and insect eggs on the seed surface and inside, while breaking seed dormancy, softening the seed coat, and making it easier for water and oxygen to enter the seed.
[0015] In a preferred embodiment, in step S1, the disinfected seeds are placed in a container and warm water at 30-40°C is poured in to completely submerge the seeds. The seeds are soaked for 12-18 hours, and the soaking time may be advanced or delayed depending on the specific condition of the seeds. During the soaking period, the seeds are stirred to promote even absorption of water. After soaking, the seeds are checked to see if they have absorbed water and swelled, and whether the seed coat has softened. Finally, the seeds are rinsed with clean water and air-dried naturally.
[0016] In a preferred embodiment, in step S2, fresh Koelreuteria paniculata seed samples are collected, placed in sealed bags, and stored at low temperature. 1g of fresh Koelreuteria paniculata seeds are selected, treated with 75% alcohol for 30s, rinsed 3-5 times with sterile water, then soaked in 10% sodium hypochlorite for 10min, and rinsed 3-5 times with sterile water. Due to the hard texture of the seeds, they are soaked in phosphate buffer for 48h, then ground, and 1mL of the homogenate supernatant is diluted to 10... －1 -10 －7 100 μL of each strain was spread onto PDA, LB, and Gao's No. 1 solid plates, with each dilution gradient repeated three times. The solid plates were placed in a constant temperature incubator and incubated in the dark at 30°C. After the strains grew, strains with different colony morphologies and sizes were picked and purified using the streak plate method. After the colony morphology of the isolated strains was consistent, the strains were preserved in 30% glycerol. The preserved endophytic bacteria were cultured in a shaker at 37°C and 200 rpm for 48 h. 1 mL of the bacterial solution was then serially diluted to the appropriate concentration for later use. The adhesive was prepared according to a ratio of 5.2 g / 200 ml water. The adhesive was stirred evenly and fully dissolved, and the foam was removed by standing or centrifugation.
[0017] In a preferred embodiment, in step S3, the required mass of soaked and dried Amorpha fruticosa seeds is weighed, and an adhesive corresponding to the volume of the carrier is added. The mixture is stirred evenly, and then a suitable concentration of endophytic bacterial solution is added. The mixture is stirred again, and then maifanite and bentonite, which have been screened through a 150-200 mesh sieve, are added as carriers to quickly coat the seeds evenly. The amount of maifanite and bentonite added is determined according to the actual situation to ensure the pelleting effect.
[0018] In a preferred embodiment, in step S4, the mixture is placed into the mixer, and an appropriate amount of water is added according to the total volume of the mixed material and the dryness of the material. The mixer is started to rotate and mix evenly. After the mixture is evenly mixed, the material enters the granulator to expand and form.
[0019] In a preferred embodiment, in step S5, the shaped material is dried in a drying device, and then screened. A screening machine is used to select pellets of uniform size, and unqualified products are removed. Qualified pellets are bagged and sealed, and stored in a dry and ventilated place to avoid moisture and high temperature. A dryer can be used to remove moisture. The seed condition is checked regularly to ensure that there is no mold or pest infestation.
[0020] In a preferred embodiment, a pelleting treatment device for improving the germination rate of Amorpha fruticosa aerial seed includes a pelleting treatment apparatus for Amorpha fruticosa aerial seed and a control device electrically connected to the pelleting treatment apparatus for Amorpha fruticosa aerial seed. The control device includes a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, it implements the method as described in any of the above embodiments.
[0021] The technical effects and advantages of the present invention regarding the pelleting treatment method and equipment for improving the germination rate of Amorpha fruticosa aerial seeds are as follows:
[0022] 1. Through the synergistic effect of multi-step pretreatment and pelleting process, the germination rate and survival rate of Amorpha fruticosa seeds are significantly improved. Pelletizing optimizes the physical properties of the seeds and greatly enhances their adaptability to aerial sowing. Using maifanite and bentonite as carriers, pellets are formed by binding with adhesives, transforming the originally hard, brittle, and thin-shelled Amorpha fruticosa seeds into round particles, increasing their weight by more than 1.5 times. This reduces drift during aerial sowing and displacement after landing, achieving uniform sowing. The outer shell of the pellets can effectively prevent birds and rodents from entering the soil, reducing seed loss. When exposed to water, the pellets gradually break down to form a protective layer, enhancing moisture retention and preventing flash germination. At the same time, they are suitable for harsh site conditions such as drought and salinity, solving the problem of controlling germination time and germination rate in traditional broadcasting.
[0023] 2. Make extensive use of idle sites to carry out large-scale aerial seeding cultivation of Amorpha fruticosa forests. By cultivating Amorpha fruticosa forests, air quality can be improved, soil vegetation can be restored, soil fertility can be increased, and economic development can be promoted. Land that originally did not have forest landscape value can be transformed into forest areas with forest landscape value. At the same time, air quality and water quality can be improved, climate can be regulated and the ecological environment can be changed. Attached Figure Description
[0024] Figure 1 This is a schematic flowchart of a method for pelleting Amorpha fruticosa aerial seeds to improve germination rate according to the present invention. Detailed Implementation
[0025] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0026] Example 1
[0027] Figure 1 This invention provides a method for pelleting Amorpha fruticosa aerial seeds to improve germination rate, specifically including the following steps:
[0028] Step S1: Clean, disinfect, and soak the seeds of Amorpha fruticosa. After soaking, rinse with clean water and then air dry.
[0029] Step S2: Collect fresh seeds of Koelreuteria paniculata to prepare endophytic bacterial solution, prepare adhesive, and stir the adhesive evenly and completely to dissolve it;
[0030] Step S3: Add the Amorpha fruticosa seeds and adhesive to the mixer and mix evenly. Then add the endophytic bacterial solution and continue mixing. Next, use maifanite and bentonite as carriers to quickly coat the seeds evenly and obtain the mixture.
[0031] Step S4: Mix the mixture and then granulate it.
[0032] Step S5: The granulated material is dried and screened, and finally bagged and stored.
[0033] In step S1, first manually remove obvious impurities from the Amorpha fruticosa seeds, such as stones and leaves. Then, soak the seeds in water in a container for 2 hours to soften the attached substances. Next, gently rub the seeds with your hands to remove the pulp and impurities. Use different sieves to filter and separate the seeds from the impurities. Then, rinse the seeds with water until the water becomes clear. Finally, place the cleaned seeds on a towel or paper towel and place them in a ventilated place to dry, avoiding direct sunlight.
[0034] In step S1, the washed seeds are soaked in a 1% potassium permanganate solution for 10 minutes to kill some of the pathogens, pests and insect eggs on the surface and inside of the seeds, while breaking seed dormancy, softening the seed coat and making it easier for water and oxygen to enter the seeds.
[0035] In step S1, place the sterilized seeds into a container, pour in warm water at 30℃ to completely submerge the seeds, and soak for 12 hours. The soaking time may be advanced or delayed depending on the specific condition of the seeds. Stir the seeds during the soaking period to promote even absorption of water. After soaking, check whether the seeds have absorbed water and swelled, and whether the seed coat has softened. Finally, rinse with clean water and air dry naturally.
[0036] In step S2, fresh Koelreuteria paniculata seed samples were collected, placed in sealed bags, and stored at low temperature. 1g of fresh Koelreuteria paniculata seeds were selected, treated with 75% alcohol for 30s, rinsed three times with sterile water, then soaked in 10% sodium hypochlorite for 10min, and rinsed three times with sterile water. Due to the hard texture of the seeds, they were soaked in phosphate buffer for 48h, then ground, and 1mL of the homogenate supernatant was diluted to 10... －1 -10 －7 100 μL of each strain was spread onto PDA, LB, and Gao's No. 1 solid plates, with each dilution gradient repeated three times. The solid plates were placed in a constant temperature incubator and incubated in the dark at 30°C. After the strains grew, strains with different colony morphologies and sizes were picked and purified using the streak plate method. After the colony morphology of the isolated strains was consistent, the strains were preserved in 30% glycerol. The preserved endophytic bacteria were cultured in a shaker at 37°C and 200 rpm for 48 h. 1 mL of the bacterial solution was then serially diluted to the appropriate concentration for later use. The adhesive was prepared according to a ratio of 5.2 g / 200 ml water. The adhesive was stirred evenly and fully dissolved, and the foam was removed by standing or centrifugation.
[0037] In step S3, weigh the required mass of soaked and dried Amorpha fruticosa seeds, add an adhesive of the corresponding volume to the carrier, stir evenly, then add the prepared endophytic bacterial solution of appropriate concentration, continue stirring, and then add maifanite and bentonite that have been screened through a 150-mesh sieve as carriers to quickly coat the seeds evenly. The amount of maifanite and bentonite added should be determined according to the actual situation to ensure the pelleting effect.
[0038] In step S4, the mixture is placed into the mixer. According to the total volume of the mixed material, an appropriate amount of water is added based on the dryness of the material. The mixer is started to rotate and mix evenly. After the mixture is evenly mixed, the material enters the granulator to expand and form.
[0039] In step S5, the shaped material is dried in a drying device. After drying, it is screened. A screening machine is used to select pellets of uniform size and remove unqualified products. Qualified pellets are bagged and sealed and stored in a dry and ventilated place, avoiding moisture and high temperature. A dryer can be used to remove moisture. The seed condition is checked regularly to ensure that there is no mold or pest infestation.
[0040] A pelleting treatment device for improving the germination rate of Amorpha fruticosa aerial seed includes a pelleting treatment device for Amorpha fruticosa aerial seed and a control device electrically connected to the pelleting treatment device for Amorpha fruticosa aerial seed. The control device includes a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, it implements the method described in any of the above descriptions.
[0041] Example 2
[0042] Figure 1 This invention provides a method for pelleting Amorpha fruticosa aerial seeds to improve germination rate, specifically including the following steps:
[0043] Step S1: Clean, disinfect, and soak the seeds of Amorpha fruticosa. After soaking, rinse with clean water and then air dry.
[0044] Step S2: Collect fresh seeds of Koelreuteria paniculata to prepare endophytic bacterial solution, prepare adhesive, and stir the adhesive evenly and completely to dissolve it;
[0045] Step S3: Add the Amorpha fruticosa seeds and adhesive to the mixer and mix evenly. Then add the endophytic bacterial solution and continue mixing. Next, use maifanite and bentonite as carriers to quickly coat the seeds evenly and obtain the mixture.
[0046] Step S4: Mix the mixture and then granulate it.
[0047] Step S5: The granulated material is dried and screened, and finally bagged and stored.
[0048] In step S1, first manually remove obvious impurities from the Amorpha fruticosa seeds, such as stones and leaves. Then, soak the seeds in water in a container for 2 hours to soften the attached substances. Next, gently rub the seeds with your hands to remove the pulp and impurities. Use different sieves to filter and separate the seeds from the impurities. Then, rinse the seeds with water until the water becomes clear. Finally, place the cleaned seeds on a towel or paper towel and place them in a ventilated place to dry, avoiding direct sunlight.
[0049] In step S1, the washed seeds are soaked in a 1% potassium permanganate solution for 13 minutes to kill some of the pathogens, pests and insect eggs on the surface and inside of the seeds, while breaking seed dormancy, softening the seed coat and making it easier for water and oxygen to enter the seeds.
[0050] In step S1, place the sterilized seeds into a container, pour in warm water at 35℃ to completely submerge the seeds, and soak for 15 hours. The soaking time may be advanced or delayed depending on the specific condition of the seeds. Stir the seeds during the soaking period to promote even absorption of water. After soaking, check whether the seeds have absorbed water and swelled, and whether the seed coat has softened. Finally, rinse with clean water and air dry naturally.
[0051] In step S2, fresh Koelreuteria paniculata seed samples were collected, placed in sealed bags, and stored at low temperature. 1g of fresh Koelreuteria paniculata seeds were selected, treated with 75% alcohol for 30s, rinsed four times with sterile water, then soaked in 10% sodium hypochlorite for 10min, and rinsed four times with sterile water. Due to the hard texture of the seeds, they were soaked in phosphate buffer for 48h, then ground, and 1mL of the homogenate supernatant was diluted to 10. －6 100 μL of each strain was spread onto PDA, LB, and Gao's No. 1 solid plates, with each dilution gradient repeated three times. The solid plates were placed in a constant temperature incubator and incubated in the dark at 30°C. After the strains grew, strains with different colony morphologies and sizes were picked and purified using the streak plate method. After the colony morphology of the isolated strains was consistent, the strains were preserved in 30% glycerol. The preserved endophytic bacteria were cultured in a shaker at 37°C and 200 rpm for 48 h. 1 mL of the bacterial solution was then serially diluted to the appropriate concentration for later use. The adhesive was prepared according to a ratio of 5.2 g / 200 ml water. The adhesive was stirred evenly and fully dissolved, and the foam was removed by standing or centrifugation.
[0052] In step S3, weigh the required mass of soaked and dried Amorpha fruticosa seeds, add an adhesive of the corresponding volume to the carrier, stir evenly, then add the prepared endophytic bacterial solution of appropriate concentration, continue stirring, and then add maifanite and bentonite that have been screened through a 150-200 mesh sieve as carriers to quickly coat the seeds evenly. The amount of maifanite and bentonite added should be determined according to the actual situation to ensure the pelleting effect.
[0053] In step S4, the mixture is placed into the mixer. According to the total volume of the mixed material, an appropriate amount of water is added based on the dryness of the material. The mixer is started to rotate and mix evenly. After the mixture is evenly mixed, the material enters the granulator to expand and form.
[0054] In step S5, the shaped material is dried in a drying device. After drying, it is screened. A screening machine is used to select pellets of uniform size and remove unqualified products. Qualified pellets are bagged and sealed and stored in a dry and ventilated place, avoiding moisture and high temperature. A dryer can be used to remove moisture. The seed condition is checked regularly to ensure that there is no mold or pest infestation.
[0055] A pelleting treatment device for improving the germination rate of Amorpha fruticosa aerial seed includes a pelleting treatment device for Amorpha fruticosa aerial seed and a control device electrically connected to the pelleting treatment device for Amorpha fruticosa aerial seed. The control device includes a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, it implements the method described in any of the above descriptions.
[0056] Example 3
[0057] Figure 1 This invention provides a method for pelleting Amorpha fruticosa aerial seeds to improve germination rate, specifically including the following steps:
[0058] Step S1: Clean, disinfect, and soak the seeds of Amorpha fruticosa. After soaking, rinse with clean water and then air dry.
[0059] Step S2: Collect fresh seeds of Koelreuteria paniculata to prepare endophytic bacterial solution, prepare adhesive, and stir the adhesive evenly and completely to dissolve it;
[0060] Step S3: Add the Amorpha fruticosa seeds and adhesive to the mixer and mix evenly. Then add the endophytic bacterial solution and continue mixing. Next, use maifanite and bentonite as carriers to quickly coat the seeds evenly and obtain the mixture.
[0061] Step S4: Mix the mixture and then granulate it.
[0062] Step S5: The granulated material is dried and screened, and finally bagged and stored.
[0063] In step S1, first manually remove obvious impurities from the Amorpha fruticosa seeds, such as stones and leaves. Then, soak the seeds in water in a container for 3 hours to soften the attached substances. Next, gently rub the seeds with your hands to remove the pulp and impurities. Use different sieves to filter and separate the seeds from the impurities. Then, rinse the seeds with water until the water becomes clear. Finally, place the cleaned seeds on a towel or paper towel and place them in a ventilated place to dry, avoiding direct sunlight.
[0064] In step S1, the washed seeds are soaked in a 1% potassium permanganate solution for 15 minutes to kill some of the pathogens, pests and insect eggs on the surface and inside of the seeds, while breaking seed dormancy, softening the seed coat and making it easier for water and oxygen to enter the seeds.
[0065] In step S1, place the sterilized seeds into a container, pour in warm water at 40℃ to completely submerge the seeds, and soak for 18 hours. The soaking time may be advanced or delayed depending on the specific condition of the seeds. Stir the seeds during the soaking period to promote even absorption of water. After soaking, check whether the seeds have absorbed water and swelled and whether the seed coat has softened. Finally, rinse with clean water and air dry naturally.
[0066] In step S2, fresh Koelreuteria paniculata seed samples were collected, placed in sealed bags, and stored at low temperature. 1g of fresh Koelreuteria paniculata seeds were selected, treated with 75% alcohol for 30s, rinsed 5 times with sterile water, then soaked in 10% sodium hypochlorite for 10min, and rinsed 5 times with sterile water. Due to the hard texture of the seeds, they were soaked in phosphate buffer for 48h, then ground, and 1mL of the homogenate supernatant was diluted to 10. －7 100 μL of each strain was spread onto PDA, LB, and Gao's No. 1 solid plates, with each dilution gradient repeated three times. The solid plates were placed in a constant temperature incubator and incubated in the dark at 30°C. After the strains grew, strains with different colony morphologies and sizes were picked and purified using the streak plate method. After the colony morphology of the isolated strains was consistent, the strains were preserved in 30% glycerol. The preserved endophytic bacteria were cultured in a shaker at 37°C and 200 rpm for 48 h. 1 mL of the bacterial solution was then serially diluted to the appropriate concentration for later use. The adhesive was prepared according to a ratio of 5.2 g / 200 ml water. The adhesive was stirred evenly and fully dissolved, and the foam was removed by standing or centrifugation.
[0067] In step S3, weigh the required mass of soaked and dried Amorpha fruticosa seeds, add an adhesive of the corresponding volume to the carrier, stir evenly, then add the prepared endophytic bacterial solution of appropriate concentration, continue stirring, and then add maifanite and bentonite that have been screened through a 200-mesh sieve as carriers to quickly coat the seeds evenly. The amount of maifanite and bentonite added should be determined according to the actual situation to ensure the pelleting effect.
[0068] In step S4, the mixture is placed into the mixer. According to the total volume of the mixed material, an appropriate amount of water is added based on the dryness of the material. The mixer is started to rotate and mix evenly. After the mixture is evenly mixed, the material enters the granulator to expand and form.
[0069] In step S5, the shaped material is dried in a drying device. After drying, it is screened. A screening machine is used to select pellets of uniform size and remove unqualified products. Qualified pellets are bagged and sealed and stored in a dry and ventilated place, avoiding moisture and high temperature. A dryer can be used to remove moisture. The seed condition is checked regularly to ensure that there is no mold or pest infestation.
[0070] A pelleting treatment device for improving the germination rate of Amorpha fruticosa aerial seed includes a pelleting treatment device for Amorpha fruticosa aerial seed and a control device electrically connected to the pelleting treatment device for Amorpha fruticosa aerial seed. The control device includes a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, it implements the method described in any of the above descriptions.
[0071] The seeds of Amorpha fruticosa (purple locust) were pelleted according to Examples 1-3. Germination rate and survival rate were calculated. The control group consisted of naked seeds. The results are shown in the table below:
[0072]
[0073]
[0074] As can be seen from the table above, the pelleting treatment of Amorpha fruticosa aerial seeds using Examples 1-3 significantly improved the germination rate and survival rate, and Example 2 was the best example.
[0075] Advantages of pelleted seed broadcasting:
[0076] 1. Change the seed shape. Change from winged and flat to round, making it easier for the seeds to contact the soil surface during sowing;
[0077] 2. Increased seed weight. After pelleting, the seed weight generally increases by more than 1.5 times. This speeds up the seed landing speed during broadcasting, reduces seed drift during aerial sowing and displacement after landing, and makes it easier to achieve uniform seed distribution.
[0078] 3. Improved bird repellency. Seed treatment effectively prevents damage from birds and rodents, enhances drought resistance, and improves seed germination rate and survival rate after sowing.
[0079] 4. Better moisture retention. After sowing, the part of the seed attached to the surface begins to break down when it comes into contact with water, forming a protective layer on the soil, which improves the moisture retention effect and is more conducive to seed germination.
[0080] 5. Reduces seed "snap germination". Pelleted seeds only germinate when rainfall reaches a certain level, effectively avoiding the "snap germination" phenomenon of naked seeds and improving seed germination ability.
[0081] 6. More conducive to precision sowing. After the seeds are pelleted, a tough outer shell is formed on the outside of the seeds, which increases the volume of the small seeds and is more conducive to field production and precision sowing. Manual sowing allows for better selection of sowing area and seed quantity, is easier to control in terms of timing, and is also simpler and more convenient in terms of organization.
[0082] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.
[0083] In conclusion, the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A method for pelleting Amorpha fruticosa aerial seeds to improve germination rate, characterized in that, Includes the following steps: Step S1: Clean, disinfect, and soak the seeds of Amorpha fruticosa. After soaking, rinse with clean water and then air dry. Step S2: Collect fresh seeds of Koelreuteria paniculata to prepare endophytic bacterial solution, prepare adhesive, and stir the adhesive evenly and completely to dissolve it; Step S3: Add the Amorpha fruticosa seeds and adhesive to the mixer and mix evenly. Then add the endophytic bacterial solution and continue mixing. Next, use maifanite and bentonite as carriers to quickly coat the seeds evenly and obtain the mixture. Step S4: Mix the mixture and then granulate it. Step S5: The granulated material is dried and screened, and finally bagged and stored.
2. The method for pelleting Amorpha fruticosa aerial seeds to improve germination rate according to claim 1, characterized in that: In step S1, first manually remove obvious impurities from the Amorpha fruticosa seeds. Then, soak the seeds in water in a container for 2-3 hours to soften the attached substances. Next, gently rub the seeds with your hands to remove the pulp and impurities. Use different sieves to filter and separate the seeds from the impurities. Then, rinse the seeds with water until the water becomes clear. Finally, place the cleaned seeds on a towel or paper towel and place them in a ventilated place to dry, avoiding direct sunlight.
3. The method for pelleting Amorpha fruticosa aerial seeds to improve germination rate according to claim 2, characterized in that: In step S1, the washed seeds are soaked in a 1% potassium permanganate solution for 10-15 minutes to kill some of the pathogens, pests and insect eggs on the surface and inside of the seeds, while breaking seed dormancy and softening the seed coat.
4. The method for pelleting Amorpha fruticosa aerial seeds to improve germination rate according to claim 3, characterized in that: In step S1, place the sterilized seeds into a container and pour in warm water at 30-40℃ to completely submerge the seeds. Soak for 12-18 hours, adjusting the soaking time according to the specific condition of the seeds. Stir the seeds during soaking to promote even water absorption. After soaking, check whether the seeds have absorbed water and swelled and whether the seed coat has softened. Finally, rinse with clean water and air dry naturally.
5. The method for pelleting Amorpha fruticosa aerial seeds to improve germination rate according to claim 4, characterized in that: In step S2, fresh Koelreuteria paniculata seed samples were collected, placed in sealed bags, and stored at low temperature. 1g of fresh Koelreuteria paniculata seeds were selected, treated with 75% alcohol for 30 seconds, rinsed 3-5 times with sterile water, then soaked in 10% sodium hypochlorite for 10 minutes, and rinsed 3-5 times with sterile water. Due to the hard texture of the seeds, they were soaked in phosphate buffer for 48 hours, then ground, and 1mL of the homogenate supernatant was diluted to 10. －1 -10 －7 100 μL of each strain was spread onto PDA, LB, and Gao's No. 1 solid plates, with each dilution gradient repeated three times. The solid plates were placed in a constant temperature incubator and incubated in the dark at 30°C. After the strains grew, strains with different colony morphologies and sizes were picked and purified using the streak plate method. After the colony morphology of the isolated strains was consistent, the strains were preserved in 30% glycerol. The preserved endophytic bacteria were cultured in a shaker at 37°C and 200 rpm for 48 h. 1 mL of the bacterial solution was then serially diluted to the appropriate concentration for later use. The adhesive was prepared according to a ratio of 5.2 g / 200 ml water. The adhesive was stirred evenly and fully dissolved, and the foam was removed by standing or centrifugation.
6. The method for pelleting Amorpha fruticosa aerial seeds to improve germination rate according to claim 5, characterized in that: In step S3, weigh the required mass of soaked and dried Amorpha fruticosa seeds, add an adhesive of the corresponding volume to the carrier, stir evenly, then add the prepared endophytic bacterial solution of appropriate concentration, continue stirring, and then add maifanite and bentonite that have been screened through a 150-200 mesh sieve as a carrier to quickly coat the seeds evenly. The amount of maifanite and bentonite added is determined according to the actual situation.
7. The method for pelleting Amorpha fruticosa aerial seeds to improve germination rate according to claim 6, characterized in that: In step S4, the mixture is placed into the mixer. According to the total volume of the mixed material, an appropriate amount of water is added based on the dryness of the material. The mixer is started to rotate and mix evenly. After the mixture is evenly mixed, the material enters the granulator to expand and form.
8. The method for pelleting Amorpha fruticosa aerial seeds to improve germination rate according to claim 7, characterized in that: In step S5, the shaped material is dried in a drying device. After drying, it is screened. A screening machine is used to select pellets of uniform size and remove unqualified products. Qualified pellets are bagged and sealed and stored in a dry and ventilated place, avoiding moisture and high temperature. A dryer can be used to remove moisture. The seed condition is checked regularly to ensure that there is no mold or pest infestation.
9. A pelleting treatment device for Amorpha fruticosa aerial seed to improve germination rate, characterized in that, The device includes a pelleting treatment apparatus for Amorpha fruticosa seeds and a control device electrically connected to the pelleting treatment apparatus. The control device includes a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, it implements the method as described in any one of claims 1 to 8.