A fungicidal composition for controlling rice panicle rot and its preparation method

Abstract

This invention proposes a fungicide composition for controlling rice panicle rot and its preparation method, belonging to the field of plant disease control technology. The fungicide composition for controlling rice panicle rot comprises a biological fermentation broth, a chemical composition, and a carrier mixture in a mass ratio of 1:(2-3):(4-6); the biological fermentation broth includes fermentation broths of *Bacillus pilaris* and *Bacillus belesiensis*; the chemical composition includes *Amorpha fruticosa* extract, thiamine, propolis alcohol, and methionine; the carrier mixture includes chitin, diatomaceous earth, and sucrose. The novel fungicide composition prepared using this invention can solve the problem of unsatisfactory control efficacy of existing chemical agents against rice panicle rot, achieving a control efficacy of over 98%, good control effect, simple preparation method, and all components are environmentally friendly, without causing environmental or food safety problems.

Description

Technical Field

[0001] This invention belongs to the field of plant disease control technology, and in particular relates to a fungicide composition for controlling rice panicle rot and its preparation method. Background Technology

[0002] Rice is a staple food crop, one of the most important food crops for humankind, with a long history of cultivation and consumption. Its grain is rice, and nearly half the world's population relies on rice for sustenance. Rice is also a direct economic crop; besides being eaten directly, it can be used to brew alcohol and produce sugar, serving as a raw material for industry. Rice husks and straw can also be used as livestock feed.

[0003] Due to changes in climate, farming practices, increased fertilization, and variety changes, rice panicle rot has become a widespread disease in rice-growing areas across China in recent years. Rice panicle rot is a fungal disease that mainly occurs in the late heading stage, causing seedling blight, stem rot, and basal rot. Infected spikelets develop brown, water-soaked lesions that gradually spread to the entire panicle, causing it to wither, turn yellow, and the grains to shrivel and rot. White mycelium fills the space between the diseased panicle and the husks, and sometimes grayish-white mycelium also develops between the grains. This mycelium can continue to develop during storage, causing the entire panicle to dry out and rot. Rice infected with panicle rot not only affects yield but also alters the appearance of the rice, reducing its quality and severely impacting rice production and market prices. Furthermore, the toxins produced by the pathogen during transportation and storage can cause poisoning in humans and livestock, threatening their health and posing a serious food safety problem.

[0004] Currently, most methods for controlling rice panicle rot rely on chemical agents. For example, spraying with methyl thiophanate, carbendazim, tricyclazole wettable powder, triadimefon emulsifiable concentrate, carbendazim wettable powder, and mancozeb wettable powder during the late booting and heading stages of rice. However, using chemical agents to control rice panicle rot has drawbacks, including unsatisfactory control effects and the generation of pesticide residues. Therefore, this invention proposes a novel fungicidal composition for controlling rice panicle rot and its preparation method. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention proposes a bactericidal composition for preventing and controlling rice panicle rot and its preparation method.

[0006] To achieve the above objectives, the present invention provides a bactericidal composition for preventing and controlling rice panicle rot, comprising a biological fermentation broth, a chemical composition, and a carrier mixture in a mass ratio of 1:(2-3):(4-6);

[0007] The fermentation broth includes Bacillus pilaris fermentation broth and Bacillus vesiliformes fermentation broth.

[0008] The chemical composition includes Amorpha fruticosa extract, thiamine, propolis alcohol, and methionine;

[0009] The carrier mixture includes chitin, diatomaceous earth, and sucrose.

[0010] Furthermore, the volume ratio of Bacillus pilaris fermentation broth to Bacillus belyi fermentation broth in the fermentation broth is (1-2):1.

[0011] Furthermore, the mass ratio of Amorpha fruticosa extract, thiamine, propolis alcohol, and methionine in the chemical composition is 6:2:3:1.

[0012] Furthermore, the mass ratio of chitin, diatomaceous earth and sucrose in the carrier mixture is (3-4):(1-2):1.

[0013] The present invention also provides a method for preparing the aforementioned fungicidal composition for preventing and controlling rice panicle rot, comprising the following steps:

[0014] The biological fermentation broth, chemical composition, and carrier mixture are mixed evenly according to the mass ratio to obtain the bactericidal composition for preventing and controlling rice panicle rot.

[0015] Furthermore, the preparation method of the biological fermentation broth includes the following steps:

[0016] Bacillus pilaris and Bacillus belye were separately inoculated into solid culture medium for activation, and then inoculated into liquid culture medium for shaking culture to obtain Bacillus pilaris fermentation broth and Bacillus belye fermentation broth. The Bacillus pilaris fermentation broth and Bacillus belye fermentation broth were mixed to obtain the biological fermentation broth.

[0017] Furthermore, the shaking culture was conducted at a temperature of 37°C and 200 rpm for 48 hours.

[0018] Furthermore, the inoculum size in the liquid culture medium was 1.5%.

[0019] Furthermore, the method for preparing the chemical composition includes the following steps:

[0020] The seeds of Amorpha fruticosa were crushed and extracted by reflux with ethanol. The filtrate was concentrated under reduced pressure and extracted to obtain Amorpha fruticosa extract. Propolis was soaked in ethanol to obtain propolis alcohol. The Amorpha fruticosa extract, thiamine, propolis alcohol and methionine were mixed evenly to obtain the chemical composition.

[0021] Furthermore, the reflux extraction temperature is 90°C and the time is 2 hours.

[0022] Furthermore, the ratio of propolis to ethanol is 1g:(30-40)mL, and the soaking is performed at room temperature for 36 hours.

[0023] Furthermore, the method for preparing the carrier mixture includes the following steps:

[0024] Chitosan, diatomaceous earth, and sucrose are mixed evenly according to the mass ratio to obtain the carrier mixture.

[0025] The present invention also proposes the application of the fungicidal composition for preventing and controlling rice panicle rot in the biological control of rice panicle rot.

[0026] Furthermore, the fungicide composition is sprayed during the late booting stage and the heading and flowering stage of rice, respectively.

[0027] Compared with the prior art, the present invention has the following advantages and technical effects:

[0028] (1) This invention proposes a novel bactericidal composition for the prevention and control of rice panicle rot. The novel bactericidal composition prepared by this invention can solve the problem that the chemical agents used in the prior art are not effective against rice panicle rot.

[0029] (2) The bactericidal composition provided by the present invention has a significant inhibitory effect on pathogenic fungi of ear rot such as Fusarium moniliforme and Alternaria alternata, and the control effect on ear rot is over 98%, with good control effect.

[0030] (3) The bactericidal composition provided by the present invention is simple to prepare and can be applied after being diluted with water. All components are environmentally friendly and will not cause environmental or food safety problems. It has the potential to be used as a new type of biological pesticide for the prevention and control of rice panicle rot in rice production and is worthy of further research. Detailed Implementation

[0031] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0032] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0033] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0034] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be readily apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0035] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0036] This invention provides a bactericidal composition for preventing and controlling rice panicle rot, comprising a biological fermentation broth, a chemical composition, and a carrier mixture in a mass ratio of 1:(2-3):(4-6);

[0037] The fermentation broth includes Bacillus pilaris fermentation broth and Bacillus vesiliformes fermentation broth.

[0038] The chemical composition includes Amorpha fruticosa extract, thiamine (purchased from Qingdao Jiaweida Biotechnology Co., Ltd.), propolis alcohol, and methionine (purchased from Shandong Huaheng Food Ingredients Co., Ltd.);

[0039] The carrier mixture includes chitin (purchased from Xi'an Paifite Industrial Co., Ltd.), diatomaceous earth (purchased from Lingshou County Zhanteng Mineral Products Processing Plant), and sucrose (purchased from Suzhou Zhengyang Chemical Technology Co., Ltd.).

[0040] In a preferred embodiment of the present invention, the volume ratio of Bacillus pilaris fermentation broth to Bacillus belyi fermentation broth in the fermentation broth is (1-2):1.

[0041] The *Bacillus pilaris* used in the embodiments of this invention is *Bacillus pilaris* SAAS-3-B4, with accession number CCTCC NO:M 20221703, and the *Bacillus belyss* is *Bacillus belyss* NSZ-YBGJ0001, with accession number CGMCC No.14384, both purchased from the China General Microbiological Culture Collection Center.

[0042] In a preferred embodiment of the present invention, the mass ratio of Amorpha fruticosa extract, thiamine, propolis alcohol and methionine in the chemical composition is 6:2:3:1.

[0043] In a preferred embodiment of the present invention, the mass ratio of chitin, diatomaceous earth and sucrose in the carrier mixture is (3-4):(1-2):1.

[0044] This invention also provides a method for preparing the aforementioned fungicidal composition for controlling rice panicle rot, comprising the following steps:

[0045] The biological fermentation broth, chemical composition, and carrier mixture are mixed evenly according to the mass ratio to obtain the bactericidal composition for preventing and controlling rice panicle rot.

[0046] In a preferred embodiment of the present invention, the method for preparing the biological fermentation broth includes the following steps:

[0047] Bacillus pilaris and Bacillus belye were separately inoculated onto solid culture medium (LB solid medium, purchased from Shanghai Lianshuo Biotechnology Co., Ltd.) for activation, and then inoculated onto liquid culture medium (LB liquid medium, purchased from Beijing Kulaibo Technology Co., Ltd.). The cultures were cultured at 37°C and 200 rpm with shaking for 48 h. The cultures were collected by centrifugation at 2500 rpm for 10 min at room temperature, and the cells were resuspended in sterile physiological saline. Then, the cells were inoculated onto liquid culture medium (LB liquid medium) and cultured at 37°C and 200 rpm with shaking for 48 h to obtain Bacillus pilaris fermentation broth and Bacillus belye fermentation broth. The Bacillus pilaris fermentation broth and Bacillus belye fermentation broth were mixed to obtain the fermentation broth of the biological bacteria.

[0048] In a preferred embodiment of the present invention, the method for preparing the chemical composition includes the following steps:

[0049] After crushing the seeds of Amorpha fruticosa, extract them with 70wt% ethanol under reflux at 90℃ for 2 hours. Filter the mixture and repeat the extraction twice. Combine the filtrates and concentrate them under reduced pressure. Extract the Amorpha fruticosa extract with petroleum ether, ethyl acetate, and n-butanol in equal volumes. Soak propolis in ethanol at a material-to-liquid ratio of 1g:(30-40)mL at room temperature for 36 hours to obtain propolis alcohol. Mix the Amorpha fruticosa extract, thiamine, propolis alcohol, and methionine evenly to obtain the chemical composition.

[0050] In a preferred embodiment of the present invention, the method for preparing the carrier mixture includes the following steps:

[0051] Chitosan, diatomaceous earth, and sucrose are mixed evenly to obtain the carrier mixture.

[0052] This invention also proposes the application of the fungicide composition for preventing and controlling rice panicle rot in the biological control of rice panicle rot, wherein the fungicide composition is sprayed during the late booting stage and the heading and flowering stage of rice, respectively.

[0053] All raw materials used in the embodiments of this invention were purchased commercially.

[0054] When this invention relates to numerical ranges, it should be understood that the two endpoints of each numerical range and any value between the two endpoints can be selected. To avoid redundancy, the present invention describes the following preferred embodiments, and the technical solution of the present invention will be further explained through the embodiments below.

[0055] Example 1

[0056] Bacillus pilaris SAAS-3-B4 and Bacillus belye SZ-YBGJ0001 were activated by inoculating them onto LB solid medium plates, and then inoculated onto LB liquid medium. The plates were cultured at 37°C and 200 rpm for 48 h with shaking. The culture was collected by centrifugation at 2500 rpm for 10 min at room temperature, and the cells were resuspended in sterile physiological saline. The cells were then inoculated onto LB liquid medium and cultured at 37°C and 200 rpm for another 48 h with shaking. The fermentation broths of Bacillus pilaris and Bacillus belye were obtained. The fermentation broths of Bacillus pilaris and Bacillus belye were mixed at a volume ratio of 1:1 to obtain the fermentation broth of the bacteria.

[0057] After crushing the seeds of Amorpha fruticosa, extract them with 70wt% ethanol under reflux at 90℃ for 2 hours. Filter the mixture and repeat the extraction twice. Combine the filtrates and concentrate them under reduced pressure. Extract the Amorpha fruticosa extract with petroleum ether, ethyl acetate, and n-butanol in equal volumes. Soak propolis in ethanol at a material-to-liquid ratio of 1g:35mL at room temperature for 36 hours to obtain propolis alcohol. Mix the Amorpha fruticosa extract, thiamine, propolis alcohol, and methionine in a mass ratio of 6:2:3:1 to obtain a chemical composition.

[0058] Chitosan, diatomaceous earth and sucrose were mixed evenly in a mass ratio of 3:2:1 to obtain a carrier mixture.

[0059] The biological fermentation broth, chemical composition, and carrier mixture are mixed evenly at a mass ratio of 1:3:4 to obtain a bactericidal composition for controlling rice panicle rot.

[0060] Example 2

[0061] Bacillus pilaris SAAS-3-B4 and Bacillus belyss SZ-YBGJ0001 were activated by inoculating them onto LB solid medium plates, and then inoculated onto LB liquid medium. The plates were cultured at 37°C and 200 rpm for 48 h with shaking. The culture was collected by centrifugation at 2500 rpm for 10 min at room temperature, and the cells were resuspended in sterile physiological saline. The cells were then inoculated onto LB liquid medium and cultured at 37°C and 200 rpm for another 48 h with shaking. The fermentation broths of Bacillus pilaris and Bacillus belyss were obtained. The fermentation broths of Bacillus pilaris and Bacillus belyss were mixed at a volume ratio of 2:1 to obtain the fermentation broth of the bacteria.

[0062] After crushing the seeds of Amorpha fruticosa, extract them with 70wt% ethanol under reflux at 90℃ for 2 hours. Filter the mixture and repeat the extraction twice. Combine the filtrates and concentrate them under reduced pressure. Extract the Amorpha fruticosa extract with petroleum ether, ethyl acetate, and n-butanol in equal volumes. Soak propolis in ethanol at a material-to-liquid ratio of 1g:30mL at room temperature for 36 hours to obtain propolis alcohol. Mix the Amorpha fruticosa extract, thiamine, propolis alcohol, and methionine in a mass ratio of 6:2:3:1 to obtain a chemical composition.

[0063] Chitosan, diatomaceous earth and sucrose were mixed evenly in a mass ratio of 4:1:1 to obtain a carrier mixture.

[0064] The biological fermentation broth, chemical composition, and carrier mixture were mixed evenly at a mass ratio of 1:2:6 to obtain a bactericidal composition for controlling rice panicle rot.

[0065] Example 3

[0066] Bacillus pilaris SAAS-3-B4 and Bacillus belye SZ-YBGJ0001 were activated by inoculating them onto LB solid medium plates, and then inoculated onto LB liquid medium. The plates were cultured at 37°C and 200 rpm for 48 h with shaking. The culture was collected by centrifugation at 2500 rpm for 10 min at room temperature, and the cells were resuspended in sterile physiological saline. The cells were then inoculated onto LB liquid medium and cultured at 37°C and 200 rpm for another 48 h with shaking. The fermentation broths of Bacillus pilaris and Bacillus belye were obtained. The fermentation broths of Bacillus pilaris and Bacillus belye were mixed at a volume ratio of 1:1 to obtain the fermentation broth of the bacteria.

[0067] After crushing the seeds of Amorpha fruticosa, extract them with 70wt% ethanol under reflux at 90℃ for 2 hours. Filter the mixture and repeat the extraction twice. Combine the filtrates and concentrate them under reduced pressure. Extract the extracts with petroleum ether, ethyl acetate, and n-butanol in equal volumes to obtain Amorpha fruticosa extract. Soak propolis in ethanol at a material-to-liquid ratio of 1g:40mL at room temperature for 36 hours to obtain propolis alcohol. Mix the Amorpha fruticosa extract, thiamine, propolis alcohol, and methionine in a mass ratio of 6:2:3:1 to obtain a chemical composition.

[0068] Chitosan, diatomaceous earth and sucrose were mixed evenly in a mass ratio of 3:1:1 to obtain a carrier mixture.

[0069] The biological fermentation broth, chemical composition, and carrier mixture are mixed evenly at a mass ratio of 1:2:5 to obtain a bactericidal composition for controlling rice panicle rot.

[0070] Comparative Example 1

[0071] Mancozeb 80% wettable powder (purchased from Henan Tianchou Chemical Products Co., Ltd.)

[0072] Comparative Example 2

[0073] Tricyclazole wettable powder (purchased from Linyi Baoyun Agricultural Technology Co., Ltd.)

[0074] Comparative Example 3

[0075] Same as Example 1, except that the addition of the biological fermentation broth is omitted, and the chemical composition and carrier mixture are mixed evenly at a mass ratio of 3:4 to obtain the bactericidal composition.

[0076] Comparative Example 4

[0077] Same as Example 1, except that the addition of chemical composition is omitted, and the biological fermentation broth and carrier mixture are mixed evenly at a mass ratio of 1:4 to obtain the bactericidal composition.

[0078] Comparative Example 5

[0079] Same as Example 1, except that the fermentation broth does not contain Bacillus repens fermentation broth:

[0080] Bacillus belye SZ-YBGJ0001 was inoculated onto LB solid medium plates for activation, and then inoculated onto LB liquid medium. The culture was incubated at 37°C and 200 rpm for 48 h with shaking. The culture was then collected by centrifugation at 2500 rpm for 10 min at room temperature, and the cells were resuspended in sterile physiological saline. The cells were then inoculated onto LB liquid medium and incubated at 37°C and 200 rpm for another 48 h with shaking to obtain the Bacillus belye fermentation broth, which is the biological fermentation broth.

[0081] After crushing the seeds of Amorpha fruticosa, extract them with 70wt% ethanol under reflux at 90℃ for 2 hours. Filter the mixture and repeat the extraction twice. Combine the filtrates and concentrate them under reduced pressure. Extract the Amorpha fruticosa extract with petroleum ether, ethyl acetate, and n-butanol in equal volumes. Soak propolis in ethanol at a material-to-liquid ratio of 1g:35mL at room temperature for 36 hours to obtain propolis alcohol. Mix the Amorpha fruticosa extract, thiamine, propolis alcohol, and methionine in a mass ratio of 6:2:3:1 to obtain a chemical composition.

[0082] Chitosan, diatomaceous earth and sucrose were mixed evenly in a mass ratio of 3:2:1 to obtain a carrier mixture.

[0083] The biological fermentation broth, chemical composition, and carrier mixture are mixed evenly at a mass ratio of 1:3:4 to obtain a bactericidal composition.

[0084] Comparative Example 6

[0085] Same as Example 1, except that the biological fermentation broth, chemical composition and carrier mixture are mixed evenly in a mass ratio of 1:4:2 to obtain a bactericidal composition.

[0086] Performance testing

[0087] Field control trials

[0088] Rice (variety: Kongyu 131) was planted at the experimental base of the Institute of Plant Protection, Jiangxi Academy of Agricultural Sciences. The row spacing of the rice was 30cm and the plant spacing was 12cm. Normal field management was carried out.

[0089] Ten groups were set up with 50 rice plants each. Nine groups were sprayed with the fungicides of Examples 1-3 and Comparative Examples 1-6 once during the late booting stage (July 20) and the heading and flowering stage (July 9 and 12). The amount of each spray was 5 mL / plant. The fungicide was diluted with 50 times the amount of water before spraying. The other group served as a blank control and was sprayed with water.

[0090] Fusarium moniliformes and Alternaria alterniflora were prepared into 5×10⁻⁶ samples. 5 A suspension of spores / mL was injected into the glumes of rice spikelets on the day of flowering, with 1 mL of suspension inoculated into each spikelet.

[0091] On the 30th day after inoculation with suspension, 100 rice panicles were randomly selected from each group, and the disease incidence was recorded. The disease incidence of the grains was divided into 5 levels, as shown in Table 1.

[0092] Table 1 Grading Standards for Rice Ear Rot Disease

[0093] Disease classification 0 No brown spots on the grains 1 Brown spot type: Small brown spots on the grains 2 Brown-spotted type: Large brown spots on the grains. 3 Semi-brown type: Nearly half of the grain turns yellowish-brown / dark brown. 4 All-brown type: The grains turn completely yellowish-brown / dark brown.

[0094] The disease index and control effect of each group of rice were calculated according to the formula, and the results are shown in Table 2.

[0095] In the formula, "control" refers to the blank control group, and "treatment" refers to the group after treatment in the example or comparative example.

[0096] Table 2 Disease index and control effect of rice in each group

[0097]

[0098] As can be seen from Table 2, the bactericidal composition prepared in the embodiments of the present invention has a control effect of over 98% on rice panicle rot, and the control effect is good. It can solve the problem of unsatisfactory control effect of chemical agents on rice panicle rot in the prior art. At the same time, it will not cause environmental pollution or pesticide residue when used.

[0099] The above are merely preferred embodiments of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A fungicidal composition for controlling rice panicle rot, characterized in that, The mixture of the bio-bacteria fermentation liquor, the chemical composition and the carrier mixture has a mass ratio of 1:(2-3):(4-6); The bio-bacteria fermentation liquor comprises Paenibacillus peoriae fermentation liquor and Bacillus velezensis fermentation liquor; the chemical composition comprises Amorpha fruticosa extract, thiamine, propolis alcohol and methionine; the Paenibacillus peoriae fermentation liquor is Paenibacillus peoriae SAAS-3-B4 fermentation liquor, and the Paenibacillus peoriae SAAS-3-B4 has a preservation number of CCTCC NO:M 20221703; the Bacillus velezensis fermentation liquor is Bacillus velezensis NSZ-YBGJ0001 fermentation liquor, and the Bacillus velezensis NSZ-YBGJ0001 has a preservation number of CGMCC No.14384; the carrier mixture comprises chitin, diatomite and sucrose; the bio-bacteria fermentation liquor comprises Paenibacillus peoriae fermentation liquor and Bacillus velezensis fermentation liquor, and the volume ratio of the two is (1-2):1; the chemical composition comprises Amorpha fruticosa extract, thiamine, propolis alcohol and methionine, and the mass ratio of the four is 6:2:3:1; the Amorpha fruticosa extract is obtained by crushing Amorpha fruticosa seeds, extracting the crushed Amorpha fruticosa seeds with 70wt% ethanol at 90°C for 2h, filtering, repeatedly extracting for 2 times, combining the filtrates, and successively extracting the combined filtrate with petroleum ether, ethyl acetate and n-butanol in equal volumes; and the carrier mixture comprises chitin, diatomite and sucrose, and the mass ratio of the three is (3-4):(1-2):

1. The method comprises the following steps: The bio-bacteria fermentation liquor, the chemical composition and the carrier mixture are mixed according to a mass ratio to obtain the fungicidal composition for preventing and treating rice panicle rot. The preparation method of the bio-bacteria fermentation liquor comprises the following steps: Paenibacillus peoriae and Bacillus velezensis are inoculated in solid culture medium respectively for activation, and then inoculated in liquid culture medium for shaking culture to obtain Paenibacillus peoriae fermentation liquor and Bacillus velezensis fermentation liquor, and the two are mixed to obtain the bio-bacteria fermentation liquor. The preparation method of the chemical composition comprises the following steps: Amorpha fruticosa seeds are crushed, and then extracted with ethanol by refluxing, and the filtrate is concentrated under reduced pressure and extracted to obtain Amorpha fruticosa extract; propolis is soaked with ethanol to obtain propolis alcohol; and the Amorpha fruticosa extract, thiamine, propolis alcohol and methionine are mixed to obtain the chemical composition.

2. A method for preparing the fungicidal composition for controlling rice panicle rot according to claim 1, characterized by, The preparation method of the carrier mixture comprises the following steps: Chitin, diatomite and sucrose are mixed according to a mass ratio to obtain the carrier mixture.

3. The method of preparing the fungicidal composition for controlling rice panicle rot according to claim 2, characterized in that, 6. The fungicidal composition for preventing and treating rice panicle rot according to claim 1 is applied in biological prevention of rice panicle rot. The fungicidal composition is sprayed at the late booting stage and the heading and flowering stage of rice.

4. The method of preparing the fungicidal composition for controlling rice panicle rot according to claim 2, characterized in that, ​ ​ 5. The method of preparing the fungicidal composition for controlling rice panicle rot according to claim 2, characterized in that, ​ ​ ​ 7. Use according to claim 6, characterized in that, ​

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