5 results about "Isomerase Gene" patented technology

The application discloses a platycodon grandiflorum phosphoglucoisomerase gene PgGPI and an application of a product coded by the platycodon grandiflorum phosphoglucoisomerase gene PgGPI, and belongs to the technical field of platycodon grandiflorum genes. The platycodon grandiflorum phosphoglucoisomerase gene PgGPI is cloned from the platycodon grandiflorum, and a nucleotide sequence of the platycodon grandiflorum phosphoglucoisomerase gene PgGPI is shown as SEQ ID NO. 1. The PgGPI gene is successfully expressed in Escherichia coli, and it is verified through an enzymatic experiment that the PgGPI has catalytic activity and can effectively isomerize glucose-6-phosphate into fructose-6-phosphate, thereby laying a solid foundation for further exploring a biosynthesis mechanism of the platycodon grandiflorum polysaccharide. In addition, by means of the PgGPI gene and gene engineering technology, it is expected to significantly improve the content of the polysaccharide substance in the platycodon grandiflorum.

The present application relates to the field of bioengineering and genetic engineering, and particularly provides a rapid mining method of isomerase genes. The method is based on big data analysis and bioinformatics technology, combined with sequence alignment, gene expression analysis and function prediction, etc. means, realizes the rapid and accurate mining of isomerase genes, and provides strong technical support for the development and application of enzyme engineering field.

The application provides a saccharomyces cerevisiae engineering bacterium for producing squalene through endogenous and artificial synthetic pathways and an application thereof, the saccharomyces cerevisiae engineering bacterium takes ZS00 strain as a starting strain, overexpresses acetyl coenzyme A acetyltransferase gene, hydroxymethylglutaryl coenzyme A synthase gene and N-terminal truncated hydroxymethylglutaryl coenzyme A reductase gene, and effectively improves the yield of squalene. Heterologous expression of mevalonate kinase gene and mevalonate pyrophosphate decarboxylase gene, overexpression of the key gene squalene synthase gene for promoting the conversion of precursors into squalene effectively promotes the synthesis of squalene. The introduction of isopentenol utilization pathway genes and their mutants, through the regulation of IU pathway key substrate, overexpression of isopentenyl pyrophosphate isomerase gene and farnesyl pyrophosphate synthase gene, the final strain can effectively accumulate squalene to 687.93 mg / L. The application realizes the breakthrough of squalene yield and efficiency through complementation and synergistic effect.

This invention relates to a genetically engineered strain for synthesizing retinal, its construction method, and its applications. The construction method involves introducing DNA fragments of the β-carotene 15,15'-oxygenase gene, isopentenyl pyrophosphate isomerase gene, phytoene dehydrogenase gene, geranyl-geranyl pyrophosphate synthase gene, and phytoene synthase gene into *Rhizopus cylindrica* to obtain a genetically engineered strain. This strain enhances the expression of β-carotene 15,15'-oxygenase, isopentenyl pyrophosphate isomerase, phytoene dehydrogenase, geranyl-geranyl pyrophosphate synthase, and phytoene synthase in *Rhizopus cylindrica*. By selecting *Rhizopus cylindrica* as a host, introducing key synthetic genes, and achieving stable genome integration, this method yields an engineered strain capable of efficiently synthesizing retinal using glucose as a carbon source, simplifying the production process, reducing costs, and improving industrial applicability.

This invention discloses the cloning and application of a mannuronic acid C5-isomerase gene related to high-temperature response in kelp, belonging to the field of biotechnology. This invention screens out a mannuronic acid C5-isomerase (MC5E) gene related to high-temperature stress response in kelp; based on this, specific primers are designed to clone the gene from kelp. MC5E The cDNA sequence of the gene was obtained; soluble recombinant protein was successfully expressed using a prokaryotic expression system, and high-purity MC5E protein was purified; 1H NMR spectroscopy analysis showed that this recombinant protein can convert mannuronic acid (M) in alginate to guluronic acid (G), increasing the G content in alginate; quantitative real-time PCR detection showed that under high temperature stress, kelp larvae... MC5E The significantly elevated transcription level of the gene indicates its involvement in kelp's response to high-temperature stress.