The invention relates to the technical field of glucose preparation technologies, and discloses a technology for preparing N-acetyl glucosamine through enzymatic hydrolysis chitin. The technology comprises the steps that a fresh shrimp and crab shell material is taken to be cleaned and crushed, a proteineous enzyme liquid is poured for enzymolysis treatment, a filter is adopted to filter the mixedliquid, soaking is carried out through 5%-8% of NaOH and 5%-8% of hydrochloric acid, drying is carried out after cleaning, a finished chitin product is obtained, 5000 L of deionized water is used forswelling, reaction enzyme is added, inactivation is carried out, centrifugal treatment is carried out, a liquid supernatant is extracted and mixed with an ethyl alcohol solution for precipitation, vacuum drying is carried out, the crushed material is packaged and stored, and the finished N-acetyl glucosamine product is obtained. The technology for preparing the N-acetyl glucosamine through the enzymatic hydrolysis chitin has the advantages that the common fresh shrimp and crab shell material is adopted for preparing the chitin, the operation is simple, the preparation efficiency is high, andthe product quality is high; the mixed enzyme liquid is adopted for the enzymolysis of the chitin in combination with auxiliary oscillation of ultrasonic wave, so that the preparation efficiency is improved, the technological process is simplified, the material is easy to obtain, and the product quality is higher.