Method for producing N-acetyl-glucosamine by using insect chitinase to degrade solid chitin

Abstract

The invention discloses a method for producing N-acetyl-glucosamine by using insect chitinase to degrade solid chitin. According to the method, the solid chitin in a crystal state is used as a substrate to perform hydrolysis, and a composite enzyme used in the hydrolysis is chitinase H with a final concentration greater than 0.3mg / mL and chitinase I with a final concentration greater than 0.06mg / mL; and beta-N acetylhexosaminidase 1 with a final concentration greater than 0.06mg / mL can also be added. The method of the invention also comprises a pretreatment step of solid chitin, i.e., the solid chitin is crushed to serve as a substrate for producing the N-acetyl-glucosamine, the amount of a monosaccharide product is determined by virtue of a potassium ferricyanide method, the composite enzyme is used for degrading the solid chitin substrate, so that the conversion of more than 81 percent of substrate can be realized in 5 hours, and the industrialized application prospect is good.

Description

technical field [0001] The invention relates to a method for producing N-acetylglucosamine by using insect chitinase to degrade solid chitin. Background technique [0002] Chitin, also known as chitin or chitin, is a polymer bound by N-acetylglucosamine through β-1,4 glycosidic bonds. In nature, chitin is the nitrogen-containing polysaccharide with the highest annual output, about 10 billion tons, and is an important biomass resource. Chitin is an important component of the exoskeleton of arthropods such as shrimp and crab, and it is not effectively utilized as waste in the fishery production process. Its degradation products amino sugars can be widely used in medicine, food, agriculture and other fields, and have great economic and social value. Chitin can be degraded by chemical method and biological enzymatic method, but the commonly used chemical degradation method consumes a large amount of strong acid and strong alkali, which causes huge environmental pollution, and ...

AI Technical Summary

Problems solved by technology

Chitin can be degraded by chemical method and biological enzymatic method, but the commonly used chemical degradation method consumes a large amount of strong acid and strong alkali, which causes great environmental pollution, and the reaction efficiency is low. The composition of the product is complex and the quality is poor. Degradation of Chitin Replacement

[0003] One of the main problems of enzymatic degradation of chitin is that solid chitin exists in a crystalline and compact form under natural conditions, and there are a large number of hydrogen bonds between its internal molecules, which makes biological enzymes very efficient in degrading chitin. Low, limiting the application of chitin for biomass energy

In the current methods for degrading chitin, the chitinase used is mostly derived from microorganisms, but there is no method for using insect chitinase to degrade chitin to produce N-acetylglucosamine

Moreover, few of the current methods for degrading chitin clearly use crystalline solid chitin as a substrate for hydrolysis, because crystalline chitin is more difficult to be hydrolyzed than amorphous chitin, and the hydrolysis efficiency relatively low

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