Technology for preparing N-acetyl glucosamine through enzymatic hydrolysis chitin

Abstract

The invention relates to the technical field of glucose preparation technologies, and discloses a technology for preparing N-acetyl glucosamine through enzymatic hydrolysis chitin. The technology comprises the steps that a fresh shrimp and crab shell material is taken to be cleaned and crushed, a proteineous enzyme liquid is poured for enzymolysis treatment, a filter is adopted to filter the mixedliquid, soaking is carried out through 5%-8% of NaOH and 5%-8% of hydrochloric acid, drying is carried out after cleaning, a finished chitin product is obtained, 5000 L of deionized water is used forswelling, reaction enzyme is added, inactivation is carried out, centrifugal treatment is carried out, a liquid supernatant is extracted and mixed with an ethyl alcohol solution for precipitation, vacuum drying is carried out, the crushed material is packaged and stored, and the finished N-acetyl glucosamine product is obtained. The technology for preparing the N-acetyl glucosamine through the enzymatic hydrolysis chitin has the advantages that the common fresh shrimp and crab shell material is adopted for preparing the chitin, the operation is simple, the preparation efficiency is high, andthe product quality is high; the mixed enzyme liquid is adopted for the enzymolysis of the chitin in combination with auxiliary oscillation of ultrasonic wave, so that the preparation efficiency is improved, the technological process is simplified, the material is easy to obtain, and the product quality is higher.

Description

technical field [0001] The invention relates to the technical field of glucose preparation technology, in particular to a technology for preparing N-acetylglucosamine by enzymatically decomposing chitin. Background technique [0002] Chitin, also known as chitin and chitin, is widely found in the shells of crustaceans such as shrimp and crab shells, the shells of various insects, the cartilage of some higher animals, and the cell walls of fungi and microorganisms. It is one of the most abundant biomass resources on the earth. one. Chitin is an important natural high molecular polysaccharide polymer, and the product after deacetylation is called chitosan. Chitin and chitosan are widely used in food, chemical industry, medicine, agriculture and other fields. [0003] In the industry, chitin is often used to prepare N-acetylglucosamine. N-acetylglucosamine is a monomer of chitin. Chitin has a very large stock in nature, second only to cellulose. N-acetylglucosamine has impor...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a process for preparing N-acetylglucosamine by enzymolysis of chitin, which has the advantages of fast preparation of finished products and excellent preparation quality, and solves the problem of preparing N-acetylglucosamine by enzymolysis of chitin. The glucosamine process has the disadvantages of low work efficiency and poor quality of finished products, which cannot meet the needs of N-acetylglucosamine.

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