Process for preparing conjugated fatty acid

A technology of fatty acid and fatty acid salt, applied in the field of preparing conjugated fatty acid, to achieve the effect of broadening the scope of application

Inactive Publication Date: 2005-06-22
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Kepler first discovered that Butyrivibrio fibrisolvens in the rumen can isomerize linoleic acid into 9c, 11t-CLA, but since rumen bacteria are strictly anaerobic bacteria, the requirements for bacterial culture are strict, and CLA will be destroyed Reduction to saturated fatty acids, thus restricting the use of rumen bacteria to produce CLA

Method used

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  • Process for preparing conjugated fatty acid
  • Process for preparing conjugated fatty acid
  • Process for preparing conjugated fatty acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Optimization of isomerization conditions

[0023] 1.1 Effect of linoleic acid concentration on conjugated linoleic acid

[0024] CLA is the product of isomerization of linoleic acid by lactic acid bacteria, so the concentration of substrate linoleic acid is one of the most important factors determining the formation of CLA. Due to the high concentration of unsaturated fatty acids have inhibitory effect on bacterial growth and metabolism, and this inhibitory effect on G + The lactic acid bacteria are particularly obvious, so respectively add different amounts of linoleic acid in the culture medium, and measure the output of CLA after cultivating for 24 hours. It is found that the amount of CLA formed by adding 0.1% (v / v) linoleic acid is the largest ( image 3 ), when the concentration of linoleic acid was higher than 0.4%, the number of bacteria was significantly reduced.

[0025] 1.2 Effect of culture time on CLA production

[0026] At present, it is generally belie...

Embodiment 2

[0028] Comparison of Isomerization of Different Forms of Fatty Acids

[0029] The specific isomerase produced by lactic acid bacteria can isomerize 9c and 12c unsaturated fatty acids to form 9c and 11t conjugated fatty acids. In order to understand the effect of this enzyme on unsaturated fatty acid derivatives containing 9c and 12c structures, select Linoleic acid, sodium linoleate, and corn oil were used as substrates to study the level of isomerization. The three substrates were added to the MRS medium at 0.1% respectively, 10% of the strains were added, mixed evenly, and cultured at 30° C. for 42 hours. In the linoleic acid group, conjugated linoleic acid was directly extracted with n-hexane; in the sodium linoleate group, the pH was first adjusted to 2.0 with concentrated sulfuric acid, and then conjugated linoleic acid was extracted with n-hexane; in the corn oil group, sodium hydroxide was first used to After saponification and neutralization with concentrated sulfuric a...

Embodiment 3

[0037] Comparison of different isomerization methods

[0038] Isomerase in lactic acid bacteria is a catalyst for bioisomerization to form conjugated fatty acids. In order to understand the isomerization level of lactic acid bacteria in the cultivation process, lactic acid bacteria cells, and crude enzyme solution of lactic acid bacteria cells, three methods and The relationship formed by the CLA.

[0039] Isomerization method of lactic acid bacteria during cultivation: add 10% lactic acid bacteria strains and 0.1% linoleic acid to MRS medium, mix well, and culture at 30° C. for 42 hours.

[0040] Isomerization method of lactic acid bacteria: culture lactic acid bacteria in MRS medium for 24 hours, collect the bacteria by centrifugation, add pH 7.0 phosphate buffer to the washed bacteria to make a bacterial suspension, and add 0.1% linoleic acid, mix well, and stand at 30°C for 12h.

[0041] Isomerization method of crude enzyme solution of lactic acid bacteria: After culturi...

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Abstract

The invention provides a specific isomerase produced from lactic acid bacteria, wherein biological isomerization is employed for preparing conjugated aliphatic acid, the enzyme isomerizes 9c, 12c unsaturated fatty acid to form 9c, 11t conjugated aliphatic acid, such as 9c, 11t conjugated linolic acid, 9c, 11t conjugated linolenic acid. The invention also discloses the process for preparing conjugated aliphatic acid by conducting mutagenesis, modification, isomerase gene cloning to lactic acid bacteria, and various products of the prepared conjugated aliphatic acid and lactic acid bacteria product related with conjugated aliphatic acid.

Description

technical field [0001] The present invention relates to a method for preparing conjugated fatty acids. Specifically, it describes the preparation of conjugated fatty acids through bioisomerization of specific isomerases produced by lactic acid bacteria, specifically 9c, 11t conjugated linoleic acid, 9c, 11t conjugated linolenic acid. Background technique [0002] Conjugated fatty acids refer to fatty acids containing a pair of conjugated double bonds. Because of their specific physiological activities, they have attracted great attention, such as conjugated linoleic acid and conjugated linolenic acid. conjugated linoleic acid. Conjugated linoleic acid (CLA) is a set of positions (C8, C10; C9, C11; C10, C12; C11, C13) and geometry (cis, cis; cis, trans; trans, cis; trans, Trans) isomers of octadeca-conjugated dienoic acids collectively. In nature, CLA mainly exists in the meat and milk fat of ruminants, with 0.83-5.5 mg per gram of fat, and its isomers are mainly 9c, 11t-C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40
Inventor 曹郁生刘晓华
Owner NANCHANG UNIV
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