Method for avoiding microalgae photoinhibition and improving astaxanthin yield

Abstract

The invention relates to a new method for avoiding microalgae photoinhibition and improving astaxanthin yield in the culture process of microalgae. In general, a two-stage method is adopted to cultivate the microalgae to produce astaxanthin, wherein on the first stage, massive reproduction of cells and accumulation of biomass are achieved in a heterotrophic, autotrophic or mixotrophic culture mode, and on the second stage, accumulation of the astaxanthin in the cells are achieved in methods, such as light stress assisted with nutrition stress. However, when the microalgae is transformed from the firsts stage to the second stage, phenomenons, such as light stress usually occur, and accordingly the astaxanthin yield is seriously influenced. Therefore, multiple methods are adopted to control culture conditions in a transformation process, such as medium components controlling, sunshading, weak light transition, initial inoculated density improving, gradual dilution and outdoor inoculated time adjusting so that cell conditions can be adjusted and the cells can improve the ability to resist a strong light; therefore, thelight stress problem of the microalgae under the strong light is solved completely, the efficiency of the microalgae for producing the astaxanthin is improved greatly, and low cost, high efficiency and large-scale cultivation of the microalgae for producing the astaxanthin are achieved.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for regulating the cell state of microalgae and increasing the output of astaxanthin. Background technique [0002] Astaxanthin, the chemical name is 3,3'-dihydroxy-4,4'-diketonyl-β,β'-carotene, and the molecular formula is C 40 h 52 o 4 , with a relative molecular mass of 596.86, also known as astaxanthin, astaxanthin or lobster shell pigment, is a ketone carotenoid. The color is pink, fat-soluble, insoluble in water, easily soluble in organic solvents such as chloroform, acetone, benzene and carbon disulfide. The chemical structure of astaxanthin is composed of 4 isoprene units linked by conjugated double bonds, and 2 isoprene units at both ends to form a six-membered ring structure. Since the chemical structure of astaxanthin contains a long conjugated unsaturated double bond system, it is easily damaged by the action of light, heat, oxides, etc...

AI Technical Summary

Problems solved by technology

In order to overcome the defect that the astaxanthin production is unfavorable when the cell growth condition is switched from the cell growth condition to the astaxanthin accumulation condition in the two-stage culture process, the present invention designs a method for adjusting the cell state of Haematococcus pluvialis to increase the astaxanthin production. There are three ways: (1) directly induce the high-density cells obtained in culture, and then transfer to the normal second-stage induction; (2) dilute the high-density cells obtained in culture, perform low-light induction, and then transfer to to the normal second-stage induction; (3) transfer the cultured high-density cells to the normal second-stage induction, but by means of shading (including shading with transparent or opaque materials) and optimizing the time of inoculation (that is, in the Inoculate at a time point when the light intensity is weak within a day) to make the cells adapt to the stress environment, and then cancel the shading

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