Method for avoiding microalgae photoinhibition and improving astaxanthin yield

A technology of astaxanthin and photoinhibition, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as unfavorable astaxanthin production, and achieve the effect of various means and improving efficiency.

Active Publication Date: 2017-09-22
云南保山泽元藻业健康科技有限公司
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Problems solved by technology

In order to overcome the defect that the astaxanthin production is unfavorable when the cell growth condition is switched from the cell growth condition to the astaxanthin accumulation condition in the two-stage culture process, the present invention designs a method for adjusting the cell state of Haematococcus pluvialis to increase the astaxanthin production. There are three ways: (1) directly induce the high-density cells obtained in culture, and then transfer to the normal second-stage induction; (2)

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  • Method for avoiding microalgae photoinhibition and improving astaxanthin yield
  • Method for avoiding microalgae photoinhibition and improving astaxanthin yield
  • Method for avoiding microalgae photoinhibition and improving astaxanthin yield

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Effect test

Embodiment 1

[0097] Embodiment 1. Transition process makes the state of microalgae cell change

[0098] Such as figure 1 As shown, in the first stage of culture, high-density Haematococcus pluvialis cells were obtained by heterotrophic culture. Add the transition medium and water to 1L in the 1L bioreactor and steam sterilize it, then insert 12ml of high-density (26g / L) Haematococcus pluvialis heterotrophically obtained cells when the temperature drops to 25°C, Start the transition culture, maintain the temperature at 28-38°C, the air flow rate is 1vvm, artificially illuminate for 24 hours, and the light intensity on each side is about 50klx.

[0099] During cultivation, 1% carbon dioxide was fed intermittently to keep the pH at 8-9. Promote microalgae to begin to adapt to the stress environment. After 144 hours, the cells changed from chlamydospores to green cells, and the transition process ended.

[0100] figure 2 Shows the transition culture process of Haematococcus pluvialis i...

Embodiment 2

[0101] Example 2. The culture method through the transition process makes the cell dry weight increase and the astaxanthin production increase

[0102] In this example, the microalgae cells are cultured in the first stage, then undergo the transitional process of the present invention, and then conduct cell induction, and compare it with direct cell induction without the transitional process of the present invention.

[0103] image 3 Shows the comparison of Haematococcus pluvialis cells with and without the transition process in a 1L bioreactor induction culture for 10 days. Depend on image 3 It can be seen that after 10 days of light-induced culture, the dry weight of the cells after the transition process reached 1.4g / L, the astaxanthin increased to 4.6%, and the astaxanthin output reached 64mg / L. After 10 days of light-induced culture of Haematococcus pluvialis cells without the transition process, the dry weight of the cells after the transition process only reached ...

Embodiment 3

[0105] Embodiment 3. Changes in the intracellular photosynthetic system of the cells treated by the transition process

[0106] Figure 4 It shows that the difference between the intracellular photosynthetic system of the cells treated by the transition process and the control is the difference of the electron transfer efficiency (ETR) of the intracellular photosynthetic system II of each treatment (Figure A), the difference of the non-photochemical quenching (NPQ) Difference (Panel B), photosynthetic efficiency Y(II) of photosystem II (Panel C) and other non-photochemical energy (Y(NO), Diagram D) differences. The above results indicated that the efficiency of photosynthetic system II in the cell was improved through the transitional process, especially the photosynthetic system was significantly restored by the increase of nitrogen during the transitional process. The non-photochemical quenching part is relatively small, indicating that the cells have a strong ability to ...

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Abstract

The invention relates to a new method for avoiding microalgae photoinhibition and improving astaxanthin yield in the culture process of microalgae. In general, a two-stage method is adopted to cultivate the microalgae to produce astaxanthin, wherein on the first stage, massive reproduction of cells and accumulation of biomass are achieved in a heterotrophic, autotrophic or mixotrophic culture mode, and on the second stage, accumulation of the astaxanthin in the cells are achieved in methods, such as light stress assisted with nutrition stress. However, when the microalgae is transformed from the firsts stage to the second stage, phenomenons, such as light stress usually occur, and accordingly the astaxanthin yield is seriously influenced. Therefore, multiple methods are adopted to control culture conditions in a transformation process, such as medium components controlling, sunshading, weak light transition, initial inoculated density improving, gradual dilution and outdoor inoculated time adjusting so that cell conditions can be adjusted and the cells can improve the ability to resist a strong light; therefore, thelight stress problem of the microalgae under the strong light is solved completely, the efficiency of the microalgae for producing the astaxanthin is improved greatly, and low cost, high efficiency and large-scale cultivation of the microalgae for producing the astaxanthin are achieved.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for regulating the cell state of microalgae and increasing the output of astaxanthin. Background technique [0002] Astaxanthin, the chemical name is 3,3'-dihydroxy-4,4'-diketonyl-β,β'-carotene, and the molecular formula is C 40 h 52 o 4 , with a relative molecular mass of 596.86, also known as astaxanthin, astaxanthin or lobster shell pigment, is a ketone carotenoid. The color is pink, fat-soluble, insoluble in water, easily soluble in organic solvents such as chloroform, acetone, benzene and carbon disulfide. The chemical structure of astaxanthin is composed of 4 isoprene units linked by conjugated double bonds, and 2 isoprene units at both ends to form a six-membered ring structure. Since the chemical structure of astaxanthin contains a long conjugated unsaturated double bond system, it is easily damaged by the action of light, heat, oxides, etc...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 李元广万民熙章真黄建科樊飞王军
Owner 云南保山泽元藻业健康科技有限公司
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