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Tobacco carotenoid isomerase gene and its application

A technology of carotene and isomerase, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2019-07-12
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies on this gene in tobacco, so only some valuable information can be obtained from the study of CRTISO genes in other plants

Method used

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  • Tobacco carotenoid isomerase gene and its application
  • Tobacco carotenoid isomerase gene and its application
  • Tobacco carotenoid isomerase gene and its application

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] A tobacco carotenoid isomerase gene, the base sequence of which is shown in SEQ ID NO:1.

[0018] The application of the tobacco carotenoid isomerase gene, the tobacco carotenoid isomerase gene increases the tobacco carotenoid content.

[0019] A method for increasing carotenoid content in tobacco comprises the steps of interfering and silencing tobacco carotenoid isomerase gene through transient expression technology to obtain transformed tobacco plants with increased carotenoid content.

[0020] In the method for increasing the content of tobacco carotenoids, the specific nucleic acid fragment of the tobacco carotenoid gene is used as a guide sequence, and the specific nucleic acid fragment is inserted into the plant expression vector in both positive and negative directions to construct The virus-induced gene silencing carrier and RNAi carrier of the tobacco carotenoid gene.

[0021] 1. Homologous cloning and expression pattern analysis of NtCRTISO gene

[0022] Ac...

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PUM

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Abstract

The invention discloses a tobacco carotenoid isomerase gene. The base sequence of the tobacco carotenoid isomerase gene is represented by SEQIDNO:1. In tobacco, a key carotenoid synthesis gene NtCRTISO is cloned, and the gene can be inhibited to effectively improve the carotenoid content of tobacco leaf and improve the photoinhibition resistance of tobacco plants. The invention discloses an important function of the CRTISO gene in tobacco in carotenoid synthesis. The CRTISO gene plays a great role in the regulation of the composition of carotenoid. A method for improving the content of carotenoid, especially beta-carotene, in the tobacco is searched, and a good theoretic guidance is provided for the improvement of the molecules of the plants.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, the invention relates to NtCRTISO, a key gene for synthesis of tobacco carotenoids, isomerase gene and application thereof. In particular, it relates to the important role of carotenoid isomerase (CRTISO) in carotenoid synthesis and plant growth and development. Background technique [0002] The carotenoid synthesis pathway plays an important role in higher plants. The intermediate metabolites of this pathway, such as β-carotene and lutein, participate in plant photosynthesis; the final products include abscisic acid, strigolactone, etc., which are related to plant antioxidant and growth and development. Carotenoids in tobacco belong to terpene compounds, and their content can affect the appearance and internal quality of tobacco leaves. It is not only related to the color of tobacco leaves after conditioning, but also its degradation and pyrolysis products contain nearly a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/61C12N15/84A01H5/00A01H6/82
Inventor 王燃史艳梅魏攀武明珠陈千思罗朝鹏李锋金立锋魏春阳林福呈屈凌波杨军范楷陈霞郑庆霞
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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