A method to avoid photoinhibition of microalgae to improve astaxanthin production

A technology of photoinhibition and astaxanthin, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unfavorable astaxanthin production, achieve the effect of various methods and improve efficiency
CN107189946BActive Publication Date: 2022-07-01云南保山泽元藻业健康科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
云南保山泽元藻业健康科技有限公司
Publication Date
2022-07-01

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Abstract

The present invention relates to a new method for avoiding photoinhibition to improve astaxanthin production during microalgae cultivation. The cultivation of microalgae to produce astaxanthin usually adopts a two-stage method. The first stage realizes the mass reproduction of cells and the accumulation of biomass through heterotrophic, autotrophic or polytrophic methods, and the second stage adopts light stress supplemented by nutrient stress and other methods. Accumulation of intracellular astaxanthin is achieved. However, from the first stage to the second stage, the phenomenon of photoinhibition often occurs, which seriously affects the yield of astaxanthin. Therefore, the present invention regulates the cell state by controlling the culture conditions in the transformation process, such as: controlling the composition of the medium, shading, transition to low light, increasing the initial seeding density, gradually diluting, adjusting the outdoor seeding time, etc. Therefore, it fully overcomes the problem of photoinhibition of microalgae under strong light, which can greatly improve the efficiency of microalgae production of astaxanthin, and realize low-cost, high-efficiency and large-scale cultivation of microalgae to produce astaxanthin.
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Description

technical field

[0001] The invention belongs to the technical field of microalgae biology, and in particular relates to a method for adjusting the state of microalgae cells and improving the yield of astaxanthin. Background technique

[0002] Astaxanthin, the chemical name is 3,3'-dihydroxy-4,4'-diketo-β,β'-carotene, the molecular formula is C40H52O4, the relative molecular mass is 596.86, also known as astaxanthin , astaxanthin, or lobster shell pigment, is a keto carotenoid. It is pink in color, fat-soluble, insoluble in water, and easily soluble in organic solvents such as chloroform, acetone, benzene and carbon disulfide. The chemical structure of astaxanthin is composed of 4 isoprene units linked by a conjugated double bond, and 2 isoprene units at both ends to form a six-membered ring structure. Since the chemical structure of astaxanthin contains a long conjugated unsaturated double bond system, it is easily damaged by light, heat, oxides, etc. The chemical structu...

Claims

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