A method for alleviating the photoinhibition of Haematococcus pluvialis

A technology of Haematococcus pluvialis and photoinhibition, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., to achieve the effects of high cell density, low price, and various adding methods

Active Publication Date: 2021-06-01
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no effective solution in production except for shading adjustment to prevent the occurrence of photoinhibition, and it is urgent to find a new way to solve it

Method used

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  • A method for alleviating the photoinhibition of Haematococcus pluvialis
  • A method for alleviating the photoinhibition of Haematococcus pluvialis
  • A method for alleviating the photoinhibition of Haematococcus pluvialis

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Experimental program
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Effect test

Embodiment 1

[0021] At a light intensity of 200μmol / m 2 Cultivate Haematococcus pluvialls cells under the condition of 14h / 10h light / dark ratio and reach the plateau stage, when the cell density is 502,400 cells / mL, dilute the Haematococcus pluvialls cells to 197,400 cells with tap water Cells / mL, add sodium acetate to a final concentration of 0.01mol / L, at a light intensity of 2800μmol / m 2 / s and the light-dark ratio of 14h / 10h induced the accumulation of astaxanthin. Such as figure 1 As shown, compared with the control group (no sodium acetate group), one-time addition of sodium acetate significantly reduced the degree of photoinhibition of Haematococcus pluvialis (the degree of decline in Fv / Fm).

Embodiment 2

[0023] At a light intensity of 200μmol / m 2 / s, light-dark ratio of 14h / 10h under the condition of culturing Haematococcus pluvialis cells reached the plateau stage, at this time the cell density was 513,600 cells / mL, and the Haematococcus pluvialls cells were diluted to 184,700 cells with tap water Cells / mL, add glycerol to a final concentration of 0.01mol / L, under a light intensity of 2000μmol / m 2 / s and the light-dark ratio of 14h / 10h induced the accumulation of astaxanthin. Such as figure 2 As shown, compared with the control group (without adding glycerol), the one-time addition of glycerin significantly reduced the degree of photoinhibition (decrease degree of Fv / Fm) of Haematococcus pluvialis.

Embodiment 3

[0025] At a light intensity of 160μmol / m 2 / s, light-dark ratio of 14h / 10h under the conditions of culturing Haematococcus pluvialis cells to reach the plateau stage, at this time the cell density is 497,100 cells / mL, dilute the Haematococcus pluvialls cells to 200,400 cells with tap water Cells / mL, add glycerol to a final concentration of 0.005mol / L, under a light intensity of 2500μmol / m 2 / s and the light-dark ratio of 14h / 10h induced the accumulation of astaxanthin. Such as image 3 As shown, compared with the control group (without adding glycerol), the one-time addition of glycerin significantly reduced the degree of photoinhibition (decrease degree of Fv / Fm) of Haematococcus pluvialis.

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Abstract

The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for alleviating the photoinhibition of Haematococcus pluvialis. When the culture of Haematococcus pluvialis cells reaches the plateau stage, when the light is transferred to strong light for light induction, small molecule organic matter glycerol or sodium acetate is added to the culture system, thereby reducing the photoinhibition of algal cells and promoting the production of astaxanthin during the culture process. accumulation. The present invention enhances the ability of Haematococcus pluvialls to resist strong light by adding small molecular organic matter (glycerol or sodium acetate) one or more times during the transition of the cultivation stage, and fully overcomes the strong light resistance of Haematococcus pluvialls. The occurrence of photoinhibition leads to a decrease in cell density, which in turn increases the production efficiency of astaxanthin in Haematococcus pluvialis.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for alleviating the photoinhibition of Haematococcus pluvialis. Background technique [0002] Natural astaxanthin is a safe and efficient coloring agent and strong antioxidant. It has great application potential and broad market prospects in the fields of aquatic products, feed, medicine, food and advanced health products. Haematococcus pluvialis is the organism with the highest astaxanthin content in nature, accounting for about 1-5% of the dry cell weight, so it is regarded as the "concentrate" and the best biological source of natural astaxanthin. Haematococcus pluvialis astaxanthin has attracted the attention of scholars at home and abroad for its high quality and high biological activity, and has become a research hotspot in the production of natural astaxanthin in the world. Cultivating Haematococcus pluvialis has become the best way to obtain n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/36C12N1/12C12R1/89
CPCC12N1/12C12N1/36
Inventor 张立涛刘建国张春辉
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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