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Arachis hypogaca violaxanthin de-epoxidase (AhVDE) gene and coding protein and application thereof

A technology of cyclooxygenase and violaxanthin, which is applied in the biological field to achieve obvious effects, improve the level of deepoxidation, and protect the photosystem

Inactive Publication Date: 2014-04-02
BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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Problems solved by technology

At present, there is no report on VDE gene cloning and its function research in peanut

Method used

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  • Arachis hypogaca violaxanthin de-epoxidase (AhVDE) gene and coding protein and application thereof
  • Arachis hypogaca violaxanthin de-epoxidase (AhVDE) gene and coding protein and application thereof
  • Arachis hypogaca violaxanthin de-epoxidase (AhVDE) gene and coding protein and application thereof

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Effect test

Embodiment 1

[0034] 1. Amplified peanut violaxanthin decycloxygenase ( AhVDE )

[0035] RNA was extracted from peanut leaves with RNA kit (purchased from Tiangen Biochemical Technology Co., Ltd.) and reverse transcribed. According to the highly conserved regions of known homologous genes, degenerate primers (5'-CCTGAYGARACKGAATGTC-3', 5'-TACCAGTCATCYTGRTAGTG-3') were designed for polymerase chain reaction (PCR), and the PCR products were analyzed by agarose gel The amplified fragment detected by electrophoresis was consistent with the estimated fragment size (489 bp) (see figure 1 A).

[0036] The obtained PCR product was connected with pMD18-T cloning vector, and transformed into Escherichia coli. The positive clones were quickly screened by colony PCR, the plasmids of the positive clones were extracted, and the plasmids were cut using the enzyme cutting sites inside the vector to obtain bands with the same size as the PCR products. Send the identified bacterial liquid to Shanghai ...

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Abstract

The invention relates to the field of biotechnology, and particularly relates to an arachis hypogaca violaxanthin de-epoxidase (AhVDE) gene. A nucleotide sequence is shown by the sequence 1 in a sequence table. An amino acid sequence of the AhVDE gene is shown by the sequence 2 in the sequence table. The invention also discloses an application of the AhVDE gene in a protective photosynthesis mechanism. The AhVDE gene is transfected to tobacco to obtain a transgenic plant; under the stress of excess luminous energy, the transgenic plant has higher NPQ and de-epoxidation state of xanthophyll cycle than a wild plant, thereby indicating that the over-expression of AhVDE changes the relative content of xanthophyll cycle components and leads to the change of the de-epoxidation state under the condition of excess luminous energy, and the heat dissipation capacity of xanthophyll cycle is enhanced. The capacity of the transgenic plant for dissipating the excess luminous energy is enhanced, so that the PSII excitation energy pressure is reduced, the photoinhibition degree of PSII and PSI under the stress of excess luminous energy is relieved, and the photosynthesis mechanism is protected finally.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to peanut violaxanthin decycloxygenase gene ( AhVDE ) and amino acid sequence and its application in photosystem protection. Background technique [0002] Peanut is an important oil crop and economic crop, which plays an important role in my country's agricultural production and even the entire national economy. High temperature and strong light often cause photoinhibition, which affects the accumulation of photosynthetic products in peanuts, which in turn affects the development of pods and reduces yield. With the continuous deterioration of the environment, how to improve the ability of crops to resist light damage has become one of the main goals. The current rapid development of genetic engineering technology provides a new way for plant genetic improvement, genetic transformation using genes that play an important role in the heat dissipation process is an important means to o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/11A01H5/00
Inventor 杨莎万书波李新国郭峰孟静静张佳蕾黄超杨连群
Owner BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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