Induced proliferation method for glehnia littoralis hairy roots

Abstract

The invention provides an induced proliferation method for glehnia littoralis hairy roots. The method comprises the following steps: obtaining glehnia littoralis aseptic seedlings; culturing agrobacterium rhizogenes for infection; performing co-culture on the transformed explant; and inducing hairy root generation and hairy root culture proliferation. The formation of glehnia littoralis hairy roots is induced by utilizing the agrobacterium rhizogenes infection, an in-vitro hairy root culture system is established, the content of medicinal component coumarin is analyzed and measured, the content of medicinal component coumarin is contrasted with the content of coumarin in adventitious root and artificially cultured glehnia littoralis roots, the content of coumarin in the hairy roots is the highest, and the glehnia littoralis hairy roots has the advantages of fast growth, short period, relatively high yield of the secondary metabolite and the like and can be used for industrial production of glehnia littoralis coumarin; and moreover, a novel path is provided for industrial production of the secondary metabolite of the glehnia littoralis by utilizing the hairy roots in the future, the threatened plant is protected, and the ecological balance is maintained.

Description

Technical field [0001] The invention belongs to the field of plant tissue culture, and particularly relates to a method for inducing the proliferation of coral coral hairy roots. Background technique [0002] Plant tissue culture includes organ culture, callus culture, shoot tip tissue culture, cell culture, and protoplast culture. Among them, callus culture is the most common, because in addition to shoot apex meristem culture and a few organ cultures, other types have to go through the callus stage to produce regenerated plants. The principle is to use the pluripotency of plant cells and the growth of callus Formation plays a vital role in tissue culture technology. The active ingredients in many medicinal plants can be synthesized directly from callus tissue. [0003] Coral vegetable is a perennial herb of the Umbelliferae family that grows on the beaches of the North Pacific coast. In my country, it is distributed in Liaoning, Shandong, Jiangsu and other places. It grows on b...

AI Technical Summary

Problems solved by technology

However, at present, the induction and production of the active substances of coral dish are still at the level of using conventional tissue culture and cell culture methods, and no one has studied the induction of hairy roots of coral dish

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