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A method for increasing the content of flavonoids in Scutellaria baicalensis

A technology of Scutellaria baicalensis and compound, applied in chemical instruments and methods, botanical equipment and methods, horticultural methods, etc., can solve problems such as successful exploration and immature technology, and achieve simple operation, stable cultivation system, new The effect of high-quality sustainable sources of medicine

Inactive Publication Date: 2016-05-25
雷桅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bioreactor cultivation of hairy roots to produce secondary metabolites is an emerging hotspot. Although the technology is not yet mature, there are many successful explorations

Method used

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  • A method for increasing the content of flavonoids in Scutellaria baicalensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of Elicitors and Biochemical Treatment of Hairy Roots of Scutellaria baicalensis

[0027] First prepare the Pr(NO 3 ) 3 Mother liquor, the concentration is 1mmol / L, and the pH value is adjusted to be 5.5. Then cut the hairy roots of Scutellaria baicalensis into about 2-3cm long segments, and transfer them to the elicitor biochemical medium. The formula is 1 / 2MS medium + a certain concentration (0, 5, 10, 15, 20, 25, 30mmol / L) of Pr 3+ (Pr(No 3 ) 3 、Pr 2 (SO 4 ) 3 , or PrCl 3 ), placed in 25°C±1.0°C constant temperature suspension shaking culture, rotating speed 110rpm, after 7 days, it can be seen that praseodymium can significantly promote the growth of Scutellaria baicalensis hairy roots.

Embodiment 2

[0029] Preparation of Elicitors and Biochemical Treatment of Hairy Roots of Scutellaria baicalensis

[0030] First prepare the Pr(SO 4 ) 2 Mother liquor, the concentration is 1mmol / L, and the pH value is adjusted to be 5.5. Then cut the hairy roots of Scutellaria baicalensis into about 2-3cm long segments, and transfer them to the elicitor biochemical medium. The formula is 1 / 2MS medium + a certain concentration (0, 5, 10, 15, 20, 25, 30mmol / L) of Pr 3+ (Pr(No 3 ) 3 、Pr 2 (SO 4 ) 3 , or PrCl 3 ), placed in 25°C±1.0°C constant temperature suspension shaking culture, rotating speed 110rpm, after 7 days, it can be seen that praseodymium can significantly promote the growth of Scutellaria baicalensis hairy roots.

Embodiment 3

[0032] Preparation of Elicitors and Biochemical Treatment of Hairy Roots of Scutellaria baicalensis

[0033] First prepare the PrCl mother solution, the concentration is 1mmol / L, and the pH value is adjusted to 5.5. Then cut the hairy roots of Scutellaria baicalensis into about 2-3cm long segments, and transfer them to the elicitor biochemical medium. The formula is 1 / 2MS medium + a certain concentration (0, 5, 10, 15, 20, 25, 30mmol / L) of Pr 3+ (Pr(No 3 ) 3 、Pr 2 (SO 4 ) 3 , or PrCl 3 ), placed in 25°C±1.0°C constant temperature suspension shaking culture, rotating speed 110rpm, after 7 days, it can be seen that praseodymium can significantly promote the growth of Scutellaria baicalensis hairy roots.

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Abstract

The invention relates to a method for increasing the content of flavonoids in Scutellaria baicalensis, which belongs to the field of biotechnology; adopting biochemical engineering technology, using rare earth praseodymium to induce the hairy roots of Scutellaria baicalensis to increase the content of flavonoids, which specifically includes the following steps: (1) sticking Suspension culture of hairy roots of Scutellaria baicalensis: Weigh about 100 mg of fresh hairy roots of Scutellaria baicalensis, place them in a large-scale culture container, and then add 1 / 2 MS0 medium with a volume fraction of 80% for suspension culture; (2) Pr Treatment of elicitors on the hairy root culture of Scutellaria baicalensis: add 25mmol / L Pr3+ solution to the culture medium and place it at a constant temperature of 25°C±1.0°C for 7 days; (3) Extraction and detection of flavonoids ; The present invention uses rare earth praseodymium to improve the medium, which can efficiently produce a new culture system of baicalin flavones. Compared with the 1 / 2MS0 control medium, the production of flavones increases by 4.502 times; A new high-quality sustainable drug source.

Description

technical field [0001] The invention relates to a method for increasing the content of flavonoids, in particular to a method for increasing the content of flavonoids in Scutellaria baicalensis, belonging to the field of biotechnology. Background technique [0002] Flavonoids are the medicinal plants of Labiatae Scutellaria baicalensis ( Scutellaria baicalensis Bunge) has the functions of clearing heat and detoxifying, stopping bleeding and preventing miscarriage, antibacterial and anti-inflammatory, protecting liver and gallbladder, lowering blood pressure and desensitization, sunscreen and whitening. With the continuous increase in international research on baicalin flavonoids and the gradual deepening of understanding, it is believed that it can scavenge oxygen free radicals, reduce tissue ischemia-reperfusion injury, regulate immunity, promote cell apoptosis, and anti-tumor and HIV. has an effect. In recent years, due to the extensive attention to the pharmacological ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08A01H4/00A01H5/06G01N21/78
Inventor 雷桅陈灿税晓容
Owner 雷桅
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