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Method for improving content of glycyrrhizae hairy root secondary metabolism production

A technology of secondary metabolites and hairy roots, applied in botany equipment and methods, chemicals for biological control, biocides, etc.

Inactive Publication Date: 2008-09-17
BEIJING WEIMING KAITUO CROP DESIGN CENT COMPANYLIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the detection of glycyrrhizic acid in licorice hairy roots. Some people reported that licorice hairy roots do not contain glycyrrhizic acid. For example, the research of Japan’s Gao Jingxiu et al. showed that licorice hairy roots contained glycyrrhizic acid; while some studies reported that licorice hairy roots contained glycyrrhizic acid. Glycyrrhizic acid was not detected in hairy roots, which may be related to the variety and source of licorice

Method used

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  • Method for improving content of glycyrrhizae hairy root secondary metabolism production
  • Method for improving content of glycyrrhizae hairy root secondary metabolism production

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Embodiment 1

[0020] Example 1. Treating the hairy roots of Glycyrrhizae uralensis with the method of the present invention induces the synthesis and secretion of secondary metabolites in the hairy roots.

[0021] 1. PEG induces the synthesis of secondary metabolites in the hairy roots of Glycyrrhiza Uralica

[0022] In the present embodiment, the hairy root of Glycyrrhizae uralensis is used as the material; Polyethylene glycol (PEG) with different degrees of polymerization includes PEG6000, PEG8000, PEG20000 or different concentrations of the same type of PEG (0.02g / ml-0.2g / ml ) to process licorice hairy roots. The specific operation steps are as follows:

[0023] 1. Cut off 2-3cm of the white root tip part of the vigorously growing hairy root in the induced licorice hairy root solid culture, and inoculate it into a 300ml culture bottle containing 100ml MS liquid medium. Each bottle of hairy root The inoculum size is 1g, and the MS liquid medium does not contain any hormones. The tempera...

Embodiment 2

[0038] Example 2, detection of total flavonoid content after Tween-80 treatment of licorice hairy roots

[0039] According to the method of step 1 in the step 1 of embodiment 1, the licorice hairy roots obtained by liquid culture are cultivated, and then the licorice hairy roots of this liquid culture are subcultured twice (MS medium, the temperature is 25 ℃, and the rotation speed is After growing in the dark on a shaker at 100rpm), collect all the hairy roots, remove the browned and dead hairy roots, and distribute the remaining hairy roots evenly in 300ml culture bottles. The wet weight of each bottle of hairy roots is 2g, and 100ml is added MS medium containing different concentrations of Tween-80 (0.01g / ml, 0.02g / ml, 0.03g / ml or 0.04g / ml) was induced to treat 6, 12, 24 and At 36 hours, hairy roots treated with MS medium without elicitor for the same time were used as control (ck).

[0040] According to the method detection of the step 2 of embodiment 1 the total flavonoi...

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Abstract

The invention discloses a method to improve the synthetic quantity of secondary metabolites in liquorice hairy roots; the method processes the liquorice hairy roots in a liquid medium by a matter capable of regulating the osmotic pressure of cells to form drought stress, and then liquorice hairy roots with a higher content of secondary metabolites is obtained. The matter capable of regulating the osmotic pressure of cells is PEG or tween. The method of the invention can improve the content of secondary metabolites in liquorice hairy roots by processing the liquorice hairy roots with the matter capable of regulating the osmotic pressure of cells to form drought stress. Experiment showed the method to improve the synthetic quantity of secondary metabolites in liquorice hairy roots has the advantages that, the content of total flavone in the liquorice hairy roots processed by the method is increased to 44% to 55%; the invention provides a new way to rapidly and efficiently obtain high yield liquorice effective secondary metabolites by utilizing liquorice hairy roots culture system.

Description

technical field [0001] The invention relates to a method for increasing the synthetic amount of secondary metabolites in hairy roots of licorice. Background technique [0002] Licorice is an important traditional Chinese medicine in my country. It has the functions of clearing away heat and detoxification, moisturizing the lungs and eliminating phlegm, relieving spasms and relieving pain, invigorating qi and benefiting the spleen and stomach, and harmonizing various medicines. It is known as the "king of all medicines". The main effective secondary metabolites of licorice include triterpenoid glycyrrhizic acid and licorice flavonoids, among which glycyrrhizic acid is a very precious antidote, which can prevent and treat viral hepatitis, hyperlipidemia, cancer and AIDS; Tumor, anti-inflammatory, anti-ulcer, anti-aging, liver protection and other pharmacological effects. Licorice is not only an important medicinal material, but also widely used in daily chemicals and food fie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N31/02A01P21/00
Inventor 周骅刘敬梅张海超李毅
Owner BEIJING WEIMING KAITUO CROP DESIGN CENT COMPANYLIMITED
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