Tissue culturing method for licorice adventitious root

A technology of root tissue and licorice, which is applied in the field of tissue culture of traditional Chinese medicine licorice, to achieve the effect of high yield

Inactive Publication Date: 2005-08-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there are few reports about tissue culture of licorice, and mainly focus on callus culture, and the culture of adventitious roots has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0007] Rinse the seeds of Glycyrrhizae uralensis (purchased from Beijing Shizhen Institute of Chinese Herbal Medicine) with tap water, soak them with 75% ethanol for 10 seconds, rinse them with sterile water for 2-3 times, and then soak them with 2% sodium hypochlorite solution for 15 minutes. After rinsing with sterile water for 3-4 times, inoculate the seeds in MS medium and culture for 4 days. After the buds grow to 1.1 cm, cut the middle of the roots into 0.5 cm segments and transfer them to additional 2,4- On the MS solid medium of D 2mg / L and KT 0.7mg / L, cut the formed callus into small pieces after 20 days, and transfer to the MS solid medium supplemented with IBA 2mg / L and KT 0.2mg / L After 20 days of culture, the adventitious roots in the form of fluff are formed, and then the adventitious roots continue to grow, and the adventitious roots that have grown for 20 days are transferred to the liquid medium with the same composition for culture, and the growth rate is faste...

example 2

[0009] Rinse the licorice seeds with tap water, soak them in 72% ethanol for 14 seconds, rinse them with sterile water for 2 to 3 times, then soak them in 2% sodium hypochlorite solution for 20 minutes, and rinse them with sterile water for 3 to 4 times. , the seeds were inoculated in MS medium, and cultured for 4 days. After the buds grew to 1.3 cm, the root tips were cut into small sections of 0.7 cm, and transferred to 2, 4-D 1mg / L and KT 1mg / L On MS solid medium, cut the formed callus into small pieces after 18 days, transfer to the MS solid medium supplemented with IBA 1mg / L and KT 0.1mg / L for culture, after 17 days of culture, there will be fluffy The adventitious roots were formed, and then the adventitious roots continued to grow. The adventitious roots that had grown for 23 days were transferred to the liquid medium with the same composition for culture, and the growth rate was faster.

example 3

[0011] Rinse the licorice seeds with tap water, soak them in 71% ethanol for 11 seconds, rinse them with sterile water for 2-3 times, then soak them in 2% sodium hypochlorite solution for 18 minutes, and rinse them with sterile water for 3-4 times. , inoculate the seeds in MS medium, and cultivate for 4 days. After the buds grow to 1.1-1.3cm, cut the top of the root into 0.3cm pieces, and transfer them to MS solids supplemented with IAA 3mg / L and KT 1mg / L. On the culture medium, cut the formed callus into small pieces after 15 days, transfer to the MS solid medium supplemented with IBA 3mg / L and KT 0.5mg / L for culture, and villous adventitious roots will appear after 20 days of culture After generation, the adventitious roots continue to grow, and the adventitious roots that have grown for 22 days are transferred to the liquid medium with the same composition for culture, and the growth rate is faster.

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Abstract

The present invention discloses the process of culturing adventitious root tissue of licorice, and belongs to the tissue culture of licorice as one kind of Chinese medicinal materials. The process of the present invention includes soaking licorice seed in solution with ethanol in 70-75 vol% and sodium hypochlorite in 2 vol% to sterilize and planting in MS culture medium to induce bacteria-free bud; inoculating the cut cotyledon and bud in MS culture medium containing different plant growth regulator to induce callus; transferring the callus growing for 20 days in MS culture medium with indolebutyric acid and kinin to induce adventitious root; and finally transferring the adventitious root to liquid culture medium containing the same plant growth regulator for fast growth. The present invention has simple process, high inducing rate, fast adventitious root proliferating speed and other features.

Description

Technical field: [0001] The invention discloses a tissue culture method of licorice adventitious root, which belongs to the field of tissue culture of traditional Chinese medicine licorice. Background technique: [0002] Licorice is a perennial herb of the leguminous genus Glycyrrhizae, and its roots and rhizomes are used as medicine. Modern pharmacological studies have shown that licorice has pharmacological effects such as anti-ulcer, anti-inflammatory, anti-allergic, antitussive and expectorant. At the same time, licorice is also an important sand-fixing plant in the desert and semi-desert areas of western my country. Over the years, licorice medicinal materials have mainly relied on wild resources, but in recent years due to the uncontrolled excavation of licorice, the resources of licorice in our country have been severely damaged. At present, the reserve of wild licorice is only 50% of that before the founding of the People's Republic of China. Artificial cultivation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 高文远陈海霞郭肖红陈巍
Owner TIANJIN UNIV
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