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Method for screening allotrophic nitrobacteria

A screening method, heterotrophic nitrification technology, applied in the direction of chemical instruments and methods, bacteria, water/sludge/sewage treatment, etc., can solve the problems of long generation cycle, slow growth, application limitations of autotrophic microorganisms, etc., and achieve generation cycle Short, strong adaptability, fast growth and reproduction

Inactive Publication Date: 2006-12-27
HARBIN INST OF TECH
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AI Technical Summary

Problems solved by technology

Traditional nitrous bacteria and nitric acid bacteria have a long generation cycle, extremely slow growth, and are very sensitive to environmental changes, which limits the application of autotrophic microorganisms
[0003] Compared with autotrophic nitrifying bacteria, although the decomposition efficiency of some heterotrophic bacteria is low, their quantity and growth rate in the environment are often much larger than those of autotrophic bacteria, so in some environments, heterotrophic The contribution of nitrification can be comparable to that of autotrophs, or even exceed

Method used

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Experimental program
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specific Embodiment approach 1

[0009] Specific embodiment one: present embodiment screens heterotrophic nitrifying bacteria according to the following steps:

[0010] 1. Prepare the following medium:

[0011] Beef extract peptone medium: beef extract 1g, NaCl 1g, peptone 3g, sodium acetate 1g, K 2 HPO 4 0.5g, MnSO 4 4H 2 O 0.01g, FeSO 4 0.01g, H 2 O 1000mL, pH=7.0~7.2.

[0012] Ammonia medium: NH 4 Cl 0.382g, sodium acetate 2g, MgSO 4 ·7H 2 O 0.05g, K 2 HPO 4 0.2g, NaCl 0.12g, MnSO 4 4H 2 O 0.01g, FeSO 4 0.01g, H 2 O 1000mL.

[0013] Nitrosation medium: NaNO 2 0.5g, sodium acetate 2g, MgSO 4 ·7H 2 O 0.05g, K 2 HPO 4 0.2g, NaCl 0.12g, MnSO 4 4H 2 O 0.01g, FeSO 4 0.01g, H 2 O 1000mL.

[0014] 2. Screen heterotrophic nitrifying bacteria:

[0015] 1. Microbial enrichment culture: put the water sample aside and discard the supernatant, take the activated sludge and inoculate it in the beef extract peptone liquid medium, the inoculum amount is 5-10%, at 30-35°C, 120-140rpm Cultivat...

specific Embodiment approach 2

[0018] Specific implementation mode two: this implementation mode is realized through the following steps:

[0019] 1. Microbial enrichment culture: put the water sample at rest and discard the supernatant, take the activated sludge and inoculate it in the beef extract peptone liquid medium, the inoculum amount is 5%, and cultivate at a constant temperature of 30°C and 120rpm, and there is obvious growth (visually inspect the turbidity of the culture solution to increase), then inoculate the enriched culture solution on the beef extract peptone solid medium plate, and cultivate in an incubator at 30°C for 2 to 3 days. Pick a single colony and transfer it to an inclined test tube, and then streak and purify colonies with different characters on a beef extract peptone solid medium plate, and invert the plate in an incubator for 2 to 3 days. Repeat the above operation until the separation is pure.

[0020] 2. Pick a single colony from the beef extract peptone solid medium and in...

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Abstract

A sieve method of heterotrophic nitrifying bacteria belongs to sewage treatment technical sphere. The invention is to carry out heterotrophic nitrifying phenomenon from the biology angle by screening heterotrophic nitrifying bacteria and the technology is the condition precedent to carry out short-range self-synchronous nitration and denitrification. The invention is proceeded by the following steps: 1. enriched culturing of microorganism; 2. picking out single colony from beef cream protease leather solid medium and seeding them in ammonifying culture medium to culture and purify; 3. picking out single colony from ammonifying culture medium solid medium and seeding them in nitrosation culture medium to culture and purify to obtain heterotrophic nitrifying bacteria. The heterotrophic nitrifying bacteria of this invention not only can grow and breed in culture medium using ammonia nitrogen as unique nitrogen source but also can normally grow and breed in culture medium using nitrite as unique nitrogen source, which has stronger adaptive capacity for environment and has bigger development potential in actual engineering application.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a screening method for heterotrophic nitrifying bacteria. Background technique [0002] Nitrogen pollution is one of the important causes of water eutrophication, so denitrification of sewage plays an important role in maintaining clean water and preventing eutrophication. Nitrification can be divided into two conversion processes from ammonia to nitrous acid (ammonia oxidation) and from nitrous acid to nitric acid (nitrous acid oxidation). The formed nitrate is denitrified to form nitrogen gas and escape from the water body to complete denitrification. It is generally believed that the two steps of nitrification are completed by two types of chemoautotrophic microorganisms. The nitrous bacteria that can oxidize ammonia nitrogen to nitrite are the nitrite bacteria, and the nitrite that can oxidize nitrite to nitrate are nitrate bacteria. plays a very importa...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/00
Inventor 马放苏俊峰杨基先
Owner HARBIN INST OF TECH
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