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Method for repairing cadmium-polluted soil by combination of microorganisms and plants

A cadmium-contaminated soil and joint restoration technology is applied in the field of joint restoration of cadmium-contaminated soil by microbial inoculants and plants, which can solve problems such as strong changes and achieve the effects of strong adaptability, broad application prospects and low cost.

Active Publication Date: 2015-12-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few cases of combined application of microorganisms and plants, and most of them still use a single microorganism, which is often strongly affected by environmental changes.

Method used

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  • Method for repairing cadmium-polluted soil by combination of microorganisms and plants
  • Method for repairing cadmium-polluted soil by combination of microorganisms and plants
  • Method for repairing cadmium-polluted soil by combination of microorganisms and plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Screening, identification and biological characteristics analysis of Bacillus cereus JYJ

[0030] 1) Soil sample source: Activated sludge from an electroplating sludge factory in Nanjing

[0031] 2) Strain screening: Take 1g of activated sludge and add it to 10ml of liquid LB medium (peptone 10g / L, sodium chloride 10g / L, yeast powder 5g / L.) Enrichment culture, culture conditions 37 ℃ 180r / min, cultivated for 24 hours; take 1ml of the enriched culture solution and inoculate it in the fresh liquid medium containing 100mg / L cadmium, culture at 37°C and 180r / min; / L metal ion liquid medium, increase the metal ion concentration step by step. Then use the inoculation loop to take the culture solution with the highest tolerance concentration and inoculate it on the solid plate containing 100mg / L cadmium ion, and incubate at 37℃ for 24h. According to the colony morphology on the plate, single colonies were picked and inoculated into the cadmium ion selective mediu...

Embodiment 2

[0038] A method for microorganism-plant joint restoration of cadmium-contaminated soil, in which ryegrass seeds are evenly sprinkled into cadmium-containing soil, and the seeding rate is 2-4g / m 2, and the upper part is covered with 1cm of soil. The suitable temperature of ryegrass in cadmium-contaminated soil is 15-38°C. Water regularly to keep the soil water content at 60-80% of the maximum water holding capacity in the field. After 10-20 days, when the plants germinate and grow, they will grow into the ryegrass root soil Add microbial compound bacterial agent, the dosage of microbial compound bacterial agent in soil is 0.3~1L / m 2 Utilize the absorption of ryegrass to cadmium and the intensification of microbial compound bacterial agent to jointly remove cadmium in soil; Bacillus subtilis) ATCC23857 and Shewanella (Shewanella) ATCC700550 are mixed according to the wet bacteria weight ratio of 1-10:1:1.

Embodiment 3

[0039] Example 3: The enrichment ability of cadmium in soil by different combinations of ryegrass and microbial agents

[0040] In the experiment, plastic pots with a diameter of about 10 cm were used, and each pot was filled with about 1 kg of soil. After the test soil was air-dried, it was passed through a 2 mm sieve. The heavy metal cadmium was added into the pot in the form of a solution and mixed evenly. The amount of water is 80% of the maximum water holding capacity in the field. After a week of natural balance, ryegrass seeds are sprinkled in the pots, and the seeding rate is 2g / m 2 , the upper part is covered with 1cm of soil, and after 10 days of plant growth, the experiment of each treatment group is started, and the amount of the experimental group with microbial agent is 0.4L / m 2 .

[0041] in,

[0042] Group 1 consisted of only ryegrass without any added microorganisms.

[0043] The second group is ryegrass + Bacillus cereus 0.4L / m 2 .

[0044] The third gro...

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Abstract

The invention provides a method for repairing cadmium-polluted soil by the combination of microorganisms and plants. The method comprises the following steps: uniformly scattering ryegrass seeds into cadmium-containing soil and watering to keep a moist soil condition; and after 10-20 days, adding a microorganism compound bacterial agent into ryegrass root soil when the plants sprout and grow, so as to remove cadmium in the soil by the combination of the absorption effect on the cadmium of ryegrass and the strengthening effect of the microorganism compound bacterial agent, wherein the microorganism compound bacterial agent is prepared by mixing Bacillus cereus, Bacillus subtilis and Shewanella in a wet bacterium mass ratio of (1-10) to 1 to 1. According to the method, the operation is convenient, the cost is low, the management is easy, secondary pollution cannot be caused, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of environmental remediation and relates to a method for jointly remediating cadmium-contaminated soil with microbial agents and plants. Background technique [0002] In recent years, no matter in China or in the world, controlling and mitigating the pollution and harm of heavy metals to the environment has become a focal issue. Compared with water pollution and air pollution, soil heavy metal pollution is invisible and concealed. As of 2010, more than 20 million hectares of farmland in China were polluted by heavy metals such as cadmium, chromium, zinc, lead, etc., accounting for about 1 / 5 of the total cultivated land area in my country. Every year, China loses 10 million tons of grain due to heavy metal pollution. In April 2013, the "cadmium rice" incident in the Taihu Lake Basin broke out. According to a press release issued by the Jiangsu Provincial Institute of Geological Survey, because many enterpr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/00B09C1/10
CPCB09C1/105
Inventor 谢婧婧宋天顺
Owner NANJING UNIV OF TECH
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