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High-electricity-generation shewanella bacterium and application of high-electricity-generation shewanella bacterium

A high-yielding technology for Shewanella bacteria, applied in bacteria, biochemical fuel cells, and microbial-based methods, can solve problems such as low electrical power, low electrochemical activity, and hindering the electron exchange between microorganisms and electrodes

Inactive Publication Date: 2015-01-07
THE FIRST PEOPLES HOSPITAL OF CHANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many strains capable of producing electricity, most of them have low electrochemical activity, and the electric power produced by them is relatively low
Especially for Gram-positive bacteria, not only a layer of oily membrane wrapped around the cell is a non-conductive material, but also the cell membrane is rich in some insulating substances such as peptidoglycan, which seriously hinders the electron exchange between the microorganism and the electrode, reducing the Reduces the efficiency of the MFC

Method used

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  • High-electricity-generation shewanella bacterium and application of high-electricity-generation shewanella bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] This example illustrates the method of performing the first step of plasma mutagenesis on the original strain of Shewanella.

[0050] The method for the first step plasma mutagenesis of the original strain of Shewanella is as follows:

[0051] Activation culture of Shewanella oneidensis MR-1 strain, culture temperature 28-30 ℃, 50mL centrifuge tube liquid volume 5-10mL, rotation speed 150rpm, culture time 12-16h, to obtain vigorously growing bacterial liquid; press 5v / v% was transferred to secondary, and the culture conditions were the same as for activation. Take freshly cultured cells and dilute to cell concentration OD 600 =1~1.5, drop on the sterilized and cooled slide, dry with sterile air; use helium as the discharge gas, 100W as the radio frequency power, 10SLM as the gas flow rate, and 10~1800s as the irradiation time Plasma mutagenesis was performed on the bacterial strain, and after the mutagenesis, the bacterial film on the carrier was eluted, and the surv...

Embodiment 2

[0053] This example illustrates the method for screening out high electricity producing Clostridium beijerinckii.

[0054] Wherein, the culture medium formula used is as follows:

[0055] (1) TSB seed medium: 3g TSB / 100ml water

[0056] (2) The sandwich medium is as follows:

[0057] a), solid medium: yeast powder 5g / L, peptone 10g / L, sodium chloride 10g / L, agar 15-20g / L.

[0058] b), WO 3 Agar suspension: WO 3 Cluster 5g / L, sodium chloride 10g / L, agar 20g / L.

[0059] (3) Sodium lactate minimum salt solution: sodium lactate 2.02g / L, sodium chloride 5.85g / L, hydroxyethylpiperazineethanesulfonic acid 11.91g / L, sodium hydroxide 0.3g / L, ammonium chloride 1.498g / L , potassium chloride 0.097g / L, sodium dihydrogen phosphate dihydrate 0.67g / L.

[0060] Additional trace element solution (ratio 1:1000): NTA(C 6 h 9 NO 6 ) 1.5g / L, magnesium sulfate heptahydrate 30g / L, manganese sulfate monohydrate 5g / L, sodium chloride 10g / L, ferrous sulfate heptahydrate 1g / L, calcium chloride d...

Embodiment 3

[0078]This example illustrates the electricity production experiment using 20 mM sodium lactate with Shewanella as the anode catalyst.

[0079] (1) Construction of microbial fuel cells

[0080] In this embodiment, a microbial fuel cell using Shewanella as an anode catalyst to generate electricity is established according to existing technologies and methods, as shown in the attached figure 2 As shown, it includes four parts: anode chamber, cathode chamber, proton exchange membrane and external circuit. The anode electrode and cathode electrode are PAN-based graphite soft felt (5×5cm), with titanium wire as the external circuit, the external resistance is 1000Ω, the proton exchange membrane is DuPont NafionN117, and the data collector is Keithley series.

[0081] The anolyte formula is as follows: sodium lactate 20mmol / L, ammonium chloride 0.31g / L, sodium dihydrogen phosphate dihydrate 2.772g / L, disodium hydrogen phosphate dodecahydrate 11.5g / L, potassium chloride 0.13g / L, so...

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Abstract

The invention discloses a high-electricity-generation shewanella bacterium. The high-electricity-generation shewanella bacterium is named as Shewanella oneidensis; the high-electricity-generation shewanella bacterium has been preserved in the typical culture preservation center of China; the preservation serial number of the high-electricity-generation shewanella bacterium is CCTCC No: M2014340; the preservation date is July 16, 2014. The invention further discloses application of the high-electricity-generation shewanella bacterium to electricity generation. By means of the high-electricity-generation shewanella bacterium and the application of the high-electricity-generation shewanella bacterium, a survey direction is provided for the electricity-generation capacity of the shewanella bacterium in an MFC. According to the high-electricity-generation shewanella bacterium, when 20mM sodium lactate serves as an electron donor, the maximum output voltage is as high as 449 mV and is improved by 18.2% compared with that ofan initial bacterial strain MR-1, and the maximum output power density is 160 mW / m<2> and is improved by 82.6% compared with that of the initial bacterial strain MR-1.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution biological treatment and bioenergy, and in particular relates to a Shewanella bacterium and its application in microbial fuel cell electricity production. Background technique [0002] Energy shortage is an increasingly serious problem that our country is facing today, so energy development has become a major challenge for the sustainable development of our modern society. Scientists are constantly looking for new technical solutions, among which the Microbial Fuel Cell (MFC) is one of the new devices used to generate alternative energy, and its importance is becoming increasingly apparent today. [0003] At the same time, environmental pollution caused by energy development and utilization is also a major challenge to the sustainable development of modern human society. The recycling of sewage seems to be one of the models to achieve recycling while eliminating pollution. [000...

Claims

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Application Information

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IPC IPC(8): C12N1/20H01M8/16C12R1/01
CPCC12N1/205C12R2001/01H01M8/16Y02E60/50
Inventor 朱大伟郑晓孙梦茹
Owner THE FIRST PEOPLES HOSPITAL OF CHANGZHOU
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