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Algicidal Shewanella and application thereof in controlling blue algae water bloom

A technology of Shewanella and cyanobacteria blooms, applied in the field of environmental microorganisms, can solve the problems of limited processing capacity, secondary pollution, etc., and achieve the effects of short preparation cycle, good lethal effect, and good algae-dissolving effect

Inactive Publication Date: 2015-07-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the limited processing capacity of physical and chemical methods in the prior art, and the defects of easily causing secondary pollution, the technical problem to be solved in the present invention is to find efficient algae-dissolving bacteria and to separate and enrich the highly efficient algae-dissolving active substances produced by the metabolism of algae-dissolving bacteria , for safe and efficient control of cyanobacterial blooms

Method used

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  • Algicidal Shewanella and application thereof in controlling blue algae water bloom
  • Algicidal Shewanella and application thereof in controlling blue algae water bloom
  • Algicidal Shewanella and application thereof in controlling blue algae water bloom

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Experimental program
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Effect test

Embodiment 1

[0036] The screening of embodiment 1 algae-lytic bacteria

[0037] Add 10mL of natural water samples collected from the Meiliang Bay waters of Taihu Lake to 90mL of algae liquid of Microcystis aeruginosa 9110 in the logarithmic phase, take the yellow algae liquid two days later, apply the gradient dilution method to the beef extract peptone medium agar plate, and cultivate at 28°C After 24 hours, take a plate with a moderate colony density, and select different strains according to the different colony shapes.

[0038] The screened strains were inoculated into 10 mL of beef extract peptone medium, cultured at 28°C and 200 rpm for 24 hours, and 10 mL of cultured bacterial liquid was added to 90 mL of algae liquid of Microcystis aeruginosa in logarithmic phase. In addition, 10 mL of sterilized beef extract peptone medium was also added to 90 mL of algae liquid as a control. All the algae fluids of the experimental group and the control group were cultured in the light incubator...

Embodiment 2

[0042] The identification of embodiment 2 Shewanella Lzh-2 bacterial strains

[0043] Lzh-2, which has the strongest algicidal effect, was identified by morphological observation, staining, physiological and biochemical reactions, and 16srRNA gene sequence analysis. μm ~ 1.0μm × 1.0μm ~ 4.0μm, after 24 hours of shaker culture in liquid medium, individual cells up to 7μm. After being cultured on nutrient agar solid medium plate for 24 hours, the colony shape is round, the surface is smooth and flat, the edges are neat, colorless to light flesh red, transparent, and the size of the colony is 2mm to 3mm. After 16srRNA gene sequence analysis and homology comparison, it was known that it had 99% homology with a certain Shewanella strain in GenBank, so it was identified as Shewanella sp. and named Shewanella Lzh- 2. The strain has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Strains, the preservation number is CGMCC No.65...

Embodiment 3

[0044] Embodiment 3 Shewanella Lzh-2 fermented liquid and the preparation method of ethyl acetate extract thereof

[0045] Shewanella Lzh-2 was inoculated in sterilized beef extract peptone medium according to the inoculum amount of 1%, and cultured on a shaker at 200 rpm at 28°C for 48 hours to obtain a fermentation broth containing Shewanella Lzh-2. Add ethyl acetate into the fermentation broth at a ratio of 1:1, put it into a shaker and vibrate for 24 hours, and separate the upper layer solution, that is, the ethyl acetate extract. The ethyl acetate was evaporated to dryness, dissolved in water, and filtered through a microporous membrane with a pore size of 0.22 μm for further purification of metabolites.

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Abstract

The invention discloses an algicidal Shewanella and application thereof in controlling blue algae water bloom. The Shewanella sp. Lzh-2 (CGMCC No.6549) with significant algae-lysed activity is isolated from Tai Hu lake water, and effective algicidal components hexahydro-pyrrolo[1,2-A]pyrazine-1,4-dione and indoline-2,3-dione are isolated, purified and identified from the metabolites of the Shewanella, wherein hexahydro-pyrrolo[1,2-A]pyrazine-1,4-dione has median lethal dose LD50 to Microcystis aeruginosa 9110 of 5.7 Mug / mL and has no lethal effect to Synechococcus BN60, and indoline-2,3-dione has median lethal doses to Microcystis aeruginosa 9110 and Synechococcus BN60 of 12.5 Mug / mL and 34.2 Mug / mL respectively. The Shewanella sp. Lzh-2 can be used for developing and producing novel biological algicides and ultimately used for controlling lake blue algae bloom.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a strain of Shewanella sp. Lzh-2 with algae-dissolving activity and its application in the control of cyanobacteria blooms. Background technique [0002] In recent years, cyanobacteria blooms caused by lake eutrophication have polluted lake water systems more and more seriously, and freshwater areas such as Taihu Lake, Chaohu Lake, and Dianchi Lake have been seriously affected. Therefore, it is very necessary to explore effective ways to control the cyanobacterial biomass and inhibit the occurrence of cyanobacterial blooms. [0003] The control technology of cyanobacteria bloom can be summarized as physical method, chemical method and biological method. Physical methods such as mechanical algae removal, electromagnetic field algae removal, etc. can be used as auxiliary control measures for algal blooms, but the disadvantages are that they treat the symptoms but not th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/50C12R1/01
Inventor 杨虹李正华柳向龙潘建良谭晶
Owner SHANGHAI JIAO TONG UNIV
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