Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biological electrochemical detection method of fumaric acid

A bioelectrochemical and detection method technology, applied in the field of bioelectrochemical detection of fumaric acid, can solve the problems of high detection cost, long response time, and complicated operation process, and achieve high consistency, specificity, and high The effect of sensitivity

Active Publication Date: 2014-11-26
JIANGSU UNIV
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a new method for specific determination of fumaric acid, to solve the problems of high detection cost, expensive detection equipment, long response time, complicated operation process and poor repeatability in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological electrochemical detection method of fumaric acid
  • Biological electrochemical detection method of fumaric acid
  • Biological electrochemical detection method of fumaric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Bacteria used: Shewanella ( Shewanella oneidensis ), purchased from ATCC American Type Culture Collection, strain number ATCC700550.

[0038] (2) Obtaining the working bacteria solution: Inject 200 μL of glycerol bacteria into 45 mL of LB medium (containing tryptone 10 g / L, yeast extract 5 g / L and sodium chloride 5 g / L, pH=7.0) (Shewanella was mixed with 30% glycerol aqueous solution in equal proportions and stored in a -70°C refrigerator), and cultured at a temperature of 30°C and a shaking speed of 200 rpm for 16 h to obtain a bacterial liquid.

[0039] (3) Preparation of reaction solution for bioelectrochemical sensors: 95 mL of M9 medium (containing Na 2 HPO 4 .12H 2 O 17.8 g / L, KH 2 PO 4 3g / L, NaCl 0.5g / L, NH 4 Cl 1 g / L) and 5 mL LB liquid medium (containing tryptone 10 g / L, yeast extract 5 g / L and sodium chloride 5 g / L, pH=7.0) were mixed thoroughly, and sodium lactate, chloride Calcium, magnesium sulfate to the final concentration of 18 mM, 0.1 mM and...

Embodiment 2

[0049] It is basically the same as Example 1, but with the following changes: the working electrode is carbon felt. The test chart obtained is attached Figure 6 shown.

Embodiment 3

[0051] It is basically the same as Example 1, but with the following changes: the applied voltage of the working electrode is -0.4 volts. Current signal such as Figure 7 shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a biological electrochemical detection method of fumaric acid, and belongs to the technical field of biochemical detection. The method is used for specific detection of the fumaric acid, a bacteria liquid of activate cultured shewanella spp. and a buffer are mixed to use as a reaction liquid of a biochemical electrochemical sensor, a fumaric acid-containing sample is added into the reaction liquid of the biochemical electrochemical sensor, a certain electric potential is controlled, change of output current signals is detected, and the fumaric acid concentration in the sample can be calculated according to the relationship between current response and the fumaric acid concentration change. The detection method does not rely on the large-scale instrument and equipment, reduces the cost of determination and operation difficulty, is free of interference of fumaric acid structural analogues, is high in specificity, high in accuracy, fast and efficient, accurate and reliable in test results, and can be applied to biological sample detection, food and drug detection, and cancer screening and diagnosis.

Description

technical field [0001] The invention relates to a bioelectrochemical detection method for fumaric acid, belonging to the technical field of biochemical detection. Background technique [0002] Fumaric acid, also known as fumaric acid, or fumaric acid (IUPAC name (E)-butenedioic acid), is a colorless, flammable crystal, a carboxylic acid derived from butene. Its chemical formula is C 4 h 4 o 4 . Fumaric acid is an important four-carbon platform organic chemical raw material and fine chemical product. Fumaric acid and its derivatives are widely used in food, medicine, feed, chemical industry, paint, resin due to their non-toxic and low corrosion characteristics. , plasticizers and other fields. The rapid and accurate determination of its content is of great significance in the synthesis of fumaric acid and the production of its derivatives. According to reports (W. Marston Linehan & Tracey A. Rouault. Molecular Pathways: Fumarate Hydratase-Deficient Kidney Cancer: Target...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/26G01N27/30
Inventor 雍阳春高璐司荣炜翟丹丹孙建中
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com