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Shewanella putrefaciens and application thereof in organic waste treatment

A technology of Shewanella putrefaction and organic waste, which is applied in the field of agricultural biology, can solve the problems of slow and impossible heating of compost fermentation, and achieve the effects of increasing the temperature of compost fermentation, reducing losses, and shortening the fermentation cycle

Inactive Publication Date: 2013-02-13
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the technical problem of slow or even impossible heating of compost fermentation in the treatment of organic waste in small and medium-sized organic fertilizer plants under low temperature conditions in winter, the present invention makes full use of the characteristics of low-temperature microorganisms from long-term placement in low-temperature cow dung ( Cow dung samples were placed in a low-temperature refrigerator for more than 6 months) to isolate a microbial strain that can dominate the effective microbial flora in organic compost such as cow dung in a low-temperature environment, named CR7 strain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment one: the separation of cow dung composting starter bacterial classification

[0026] Collect fresh cow dung and the mix of cow dung compost (add 1% soybean meal, 1% rice bran, and 2% plant ash to the cow dung) and place them in a refrigerator at 0-4°C. After 6 months, use broth medium to isolate microbial strains , the separation method is the dilution separation method, the separation temperature is 1-4 °C, pick the microbial strains that grow a single colony within 48 hours, continue to induce culture with broth medium at low temperature, and select the microbial strains that grow a single colony within 24 hours Microbial strain number, save for future use.

Embodiment 2

[0027] Embodiment two: the mensuration of bacterial classification adaptability in sterilized cow dung medium

[0028] Example 1 The series of strains screened out were inoculated into sterilized cow dung medium, and the cultivation and formula were to add 1% soybean meal, 1% rice bran, and 2% plant ash to cow dung. Put it in a refrigerator at 0-5°C, measure the number of bacteria every 2 hours, formulate the growth curve of each strain according to the number of bacteria, and keep it within 48 hours to grow to 10 5 For the strains with more than cfu / min, select the strains that can reproduce in the shortest time to reach the maximum growth number and the highest number of bacteria reproduction. Through the experiment, a strain of milky red strain was obtained on the beef extract medium, the experimental number was CR7, and CR7 reached 1.3×10 in 16 hours. 9 cfu / ml, OD 400 It is 1.473, which is 4 hours faster than the reproduction speed of Escherichia coli CGMCC1.907 reachi...

Embodiment 3

[0029] Embodiment three: CR7 bacterial strain is measured in the survival quantity in open-air composting cow dung

[0030] The purified CR7 strain was fermented and cultured on beef extract peptone medium for 48 hours, and the fermented liquid was centrifuged at 4000r / min to retain the sediment, that is, the CR7 strain cells, which were mixed into the sterilized huller according to the volume ratio of 1:10. In bran or bran, the bacteria content is measured at 10 8 cfu / min or more, and prepared CR7 bacterial agent. According to the ratio of 0.1%, it is inoculated into fresh cow dung, mixed evenly, and composted for fermentation. Take samples under 20cm in the compost every day, and immediately use the dilution separation method in the laboratory to determine the number of CR7 strains in the samples. Beef extract peptone medium was used as the medium for separation, and the number of milky red bacterial colonies on the plate was investigated, that is, the colony number o...

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Abstract

The invention discloses a shewanella putrefaciens CR7 strain of which the preservation number is CGMCC NO.6192. The strain is separated out from cattle manure compost which is kept in a low-temperature environment for a long time; and a microbial inoculum prepared from the strain can be applied to livestock and poultry manure compost such as cattle manure and the like in winter, and has the effects of quickly starting compost fermentation, increasing compost fermentation temperature, shortening compost fermentation period and reducing nitrogen element loss. The microbial inoculum prepared from the strain is used for producing an organic fertilizer in winter, and can omit the heating procedure in the early stage of the composting of the organic fertilizer, shorten the production period of the organic fertilizer, increase the yield and lower the production cost.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to the technical application of microorganisms in the production of bio-organic fertilizers, mainly a strain of Shewanella putrefaciens and its application in organic waste treatment. technical background [0002] A large number of low-temperature bacteria are widely distributed and survive in low-temperature environments such as oceans, polar regions, glaciers, mountains, and frozen soils that account for more than 80% of the earth's surface. [ During their long-term evolution, they have established a growth mechanism that can adapt to cold environments, relying on a unique low-temperature promoter, and their RNA polymerase can perform efficient gene transcription even at 0°C, maintaining the normal growth of bacteria at low temperatures. Growth and reproduction, this kind of life activity phenomenon that is different from normal temperature bacteria and high temperature...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/00C12R1/01
CPCY02W30/40
Inventor 陈双林张磊闫淑珍
Owner NANJING NORMAL UNIVERSITY
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