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Shewanella putrefaciens capable of effectively degrading acetonitrile and application of Shewanella putrefaciens

A technology capable of degrading Shewanella putrefaciens, applied in bacteria, microbial-based methods, water/sludge/sewage treatment, etc., can solve the problems of acetonitrile compatibility research and has not yet been formed, and achieve strong adaptability , good biological stability

Active Publication Date: 2015-04-29
PETROCHINA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned studies are all acetonitrile single compound and high-concentration acetonitrile simulated wastewater degradation tests. There are few studies on the degradation ability of acetonitrile in actual sewage and the compatibility of strains with the original sewage treatment system. Process technology to improve the degradation ability of acetonitrile in sewage and meet the national discharge standard

Method used

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  • Shewanella putrefaciens capable of effectively degrading acetonitrile and application of Shewanella putrefaciens
  • Shewanella putrefaciens capable of effectively degrading acetonitrile and application of Shewanella putrefaciens
  • Shewanella putrefaciens capable of effectively degrading acetonitrile and application of Shewanella putrefaciens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the screening of efficient bacteria

[0039]The specific components and contents of the inorganic salt medium used in this example are: sodium chloride 1g / L, ammonium nitrate 1.5g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, heptahydrate Ferrous sulfate 0.02g / L, magnesium sulfate 0.1g / L, pH=7.2, the concentration of acetonitrile in the culture medium is specifically shown in each embodiment; the purity of the acetonitrile used is chromatographically pure and in solution state.

[0040] The activated sludge was taken from the outlet of the O pool of the activated sludge method treatment process in the sewage workshop of PetroChina Dalian Petrochemical Company, and the highly efficient bacteria degrading acetonitrile in the sewage of petrochemical enterprises were separated and purified by a combination of liquid enrichment culture method and dilution plate coating method . Specific steps are as follows:

[0041] (1) Add...

Embodiment 2

[0054] Embodiment 2, ZLR3 are to the degradation characteristic of acetonitrile

[0055] The sewage samples and activated sludge samples used in various experiments in this example were all taken from the sewage treatment workshop of Dalian Petrochemical Company, and acetonitrile was added to the sewage to prepare acetonitrile sewage with different concentrations.

[0056] The components and contents of the LB medium used in this example are: yeast powder 5g / L, peptone 10g / L, sodium chloride 10g / L, pH=7.2. After culturing for 18 hours under min conditions, it was used as the seed solution in the following experiments in this example (the bacteria solutions used in this example and subsequent examples are the seed solutions of each bacterium).

[0057] Experiment 1, the degradation rate of acetonitrile

[0058] Inoculate the seed solution of the strain ZLR3, DR, DLS3 screened in Example 1 to the inorganic salt liquid medium containing different concentrations of acetonitrile w...

Embodiment 3

[0075] Compatibility of embodiment 3, ZLR3 and activated sludge

[0076] Mix the activated sludge, ZLR3 seed solution and DR bacteria seed solution at a volume ratio of 1:3, 1:2, 1:1, 2:1, and 3:1, and then inoculate the mixed solution at a volume ratio of 5%. Inoculate petrochemical sewage with acetonitrile concentration of 50mg / L, and measure the COD removal rate of sewage. The preparation of the seed liquid in this embodiment is the same as that in Example 2.

[0077] The result is as Figure 7 As shown, the COD removal rate in acetonitrile wastewater is the highest after mixing activated sludge and ZLR3 seed solution at a ratio of 1:3, and the overall COD removal rate after mixing ZLR3 with activated sludge is higher than that of DR combined with activated sludge The results showed that ZLR3 was significantly better than other strains screened in this study in improving the COD removal efficiency of petrochemical wastewater containing acetonitrile.

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Abstract

The invention provides Shewanella putrefaciens capable of effectively degrading acetonitrile and application of Shewanella putrefaciens. The collection number of Shewanella putrefaciens is CGMCC No.10148. The Shewanella putrefaciens can be used for treating petrochemical enterprise sewage water having low acetonitrile concentration, and rivers, lakes and seas polluted by the petrochemical enterprise sewage water, is high in adaptability to the petrochemical enterprise sewage water, and high in biostability, and can be used for effectively removing the low-concentration acetonitrile in the petrochemical enterprise sewage water.

Description

technical field [0001] The invention relates to a Shewanella putrefaciens capable of efficiently degrading acetonitrile and its application, in particular to a high-efficiency bacterium capable of rapidly degrading acetonitrile in petrochemical sewage and its related application, and relates to the field of petrochemical sewage treatment. Background technique [0002] With the development of the petrochemical industry and the implementation of refining and chemical integration, petrochemical enterprises have more and more complex processes and more and more types of products, followed by a large amount of complex organic matter entering the sewage treatment system. The original sewage treatment system cannot fully adapt to the refractory and highly toxic organic matter, resulting in problems such as substandard effluent and prolonged hydraulic retention time. [0003] Acetonitrile, also known as methyl nitrile, is a cyanide-containing organic compound widely used in petroche...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C02F2103/365C12N1/20C12N1/205C12R2001/01
Inventor 孙珊珊闫萍罗一菁林英张敏段永鸿韩政张忠智朱宇硕张志勇张超宋昭峥刘黎斌
Owner PETROCHINA CO LTD
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