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Microbial agent for improving soil quality and preparation method and applications thereof

A microbial agent and biochar technology, applied in the field of environmental engineering, can solve the problems of high micro-environmental requirements, restricted soil quality, and long repair period, so as to avoid secondary pollution, avoid waste, and increase the content of soil organic matter.

Inactive Publication Date: 2019-04-12
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bioremediation has high requirements on the microenvironment and long repair period, which still restricts the rapid improvement of soil quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) The preparation method of microbial bacterial agent:

[0028] Activate Pseudomonas putrefaciens and Geobacter metallireducens in LB liquid medium at 37°C, 170r.min -1 , cultured under anaerobic conditions for 11 hours, Trichoderma viride and Trichoderma harzianum in liquid PDA medium at 30°C, 200r.min -1 , cultivated under aerobic conditions for 108h, Bacillus subtilis (Bacillus subtilis) in LB liquid medium at 37°C, 180r.min -1 , cultivated under aerobic conditions for 12h. After the above bacteria were collected and freeze-dried, Pseudomonas putrefaciens (S.putrefaciens): Metallireducens (G.metallireducens): Trichoderma viride (T.viride): Trichoderma harzianum (T.harzianum): subtilis Bacillus (B. subtilis) is mixed at a mass ratio of 4:3:2:2:4 to obtain a composite bacterial agent I, and the composite bacterial agent I: glucose: amino acid is mixed at a mass ratio of 75:10:15 to obtain a composite bacterial agent II

[0029] (2) The preparation method of bioch...

Embodiment 2

[0036] The microbial inoculum prepared in Example 1 was applied to the soil quality improvement test to detect the ability of the inoculum to improve soil quality. The results show that adding 20% ​​of the bacterial agent according to the mass percentage and treating it for 20 days, the organic matter content of the treatment group has increased by more than 7% compared with the control without adding the bacterial agent, increased the number of micro-aggregate particles in the soil, improved soil porosity, and has obvious advantages. improvement effect. In addition, the organic matter in the soil environment (such as polychlorinated biphenyls (PCBs) polluted farmland soil near a paper mill in Hebei (the average value of 7 indicative PCBs such as PCB28, PCB52, PCB118, PCB138, PCB153, and PCB180 was 95.82 μg / kg) , after 20 days of treatment, the average value of the above-mentioned PCB content was reduced by 51% compared with the control without adding bacterial agents) or heavy ...

Embodiment 3

[0038] The microbial bacterial agent that embodiment 1 is made is applied to Beijing Huairou district vegetable greenhouse phthalate polluted soil (dimethyl phthalate, dibutyl phthalate, butyl benzyl phthalate, o- Diethyl phthalate, bis(2-ethylhexyl) phthalate, n-octyl phthalate and other six phthalates with a total concentration of 0.58-3.26 mg / kg) to detect the bacterial agent The ability to promote the degradation of organic pollutants. The results showed that the total concentration of the six phthalates decreased by 48% in the soil repaired for 15 days by adding 20% ​​of the bacterial agent according to the mass percentage. ester degradation. In addition, the soil organic matter content of the treatment group increased by 7.3% compared with the control group.

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PUM

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Abstract

This invention relates to a microbial agent. The microbial agent is characterized by comprising functional bacteria and a protective bacterium, wherein the functional bacteria are composed of shewanella putrefaciens, geobacter metallireducens, trichoderma viride and trichoderma harzianum and the protective bacterium is bacillus subtilis. The invention also relates to a method of improving soil quality. The preparation method comprises the following steps: (1) mixing the functional bacteria, the protective bacterium and bioactive substances in proportion to obtain a composite bacterial agent; and (2) fixing the composite bacterial agent obtained in the step (1) on biochar. The microbial agent prepared by the invention has very excellent effect in improving the content of soil organic matters, is simple in method, low in cost and free from secondary pollution, can synergistically degrade soil organic matter pollution, and has broad market value.

Description

technical field [0001] The invention belongs to the field of environmental engineering, and in particular relates to a microbial agent for improving soil quality, a preparation method and application. Background technique [0002] At present, the degree and area of ​​organic pollution in my country are increasing, especially the high amount of fertilization and continuous cropping in facility agriculture, resulting in the accumulation of organic pollutants such as phthalates and polycyclic aromatic hydrocarbons year by year, which has become an important issue affecting soil quality and agricultural product quality. important factor. Microbial remediation technology can use the life activities of microorganisms to regulate or control the biochemical reactions in the habitat, and then realize the degradation and removal of organic pollutants. It has low cost and no secondary pollution, and has great application potential. However, bioremediation has high requirements on the m...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14B09C1/10C12R1/885C12R1/125C12R1/38C12R1/01
CPCC12N1/14C12N1/20B09C1/10B09C1/105
Inventor 黄彩红席北斗檀文炳袁文超杨天学夏湘勤唐朱睿
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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