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Method for repairing lead-polluted soil by combination of plants and microorganisms

A lead-contaminated soil and joint remediation technology, applied in the field of environmental remediation, can solve the problems of inability to remove heavy metals, high cost, secondary pollution, etc., and achieve the effects of no secondary pollution, easy management, and improved absorption capacity.

Active Publication Date: 2015-12-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical and chemical methods currently used to restore heavy metal-contaminated soil are expensive, damage the environment, and easily cause secondary pollution, and often cannot achieve the purpose of truly removing heavy metals.

Method used

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  • Method for repairing lead-polluted soil by combination of plants and microorganisms
  • Method for repairing lead-polluted soil by combination of plants and microorganisms
  • Method for repairing lead-polluted soil by combination of plants and microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Screening, identification and biological characteristics analysis of Bacillus mycoides ADB.

[0032] 1) Soil sample source: Activated sludge from an electroplating sludge factory in Nanjing

[0033] 2) Strain screening: Take 1g of activated sludge and add it to 10ml of liquid LB medium (peptone 10g / L, sodium chloride 10g / L, yeast powder 5g / L.) Enrichment culture, culture conditions 37 ℃ 180r / min, cultivated for 24 hours; take 1ml of the enriched culture solution and inoculate it in the fresh liquid medium containing 100mg / L lead, culture at 37°C and 180r / min; / L metal ion liquid medium, increase the metal ion concentration step by step. Then use the inoculation loop to take the culture solution with the highest tolerated concentration and inoculate it on the solid plate containing 100mg / L lead ion, and incubate at 37℃ for 24h. According to the colony morphology on the plate, single colonies were picked and inoculated into the lead ion selection medium for ...

Embodiment 2

[0039] Embodiment 2: the preparation of microbial compound bacterial agent.

[0040] The preparation method of bacterial agent in the present invention specifically comprises the following steps:

[0041] (1) Mix 5 g of beef extract, 10 g of peptone, 5 g of NaCl and 1 L of distilled water, adjust the pH value to 7.0, and sterilize to obtain a culture medium;

[0042] (2) transfer the strains preserved in the slant culture medium to inoculate the culture medium respectively, and cultivate them for 48 hours at a temperature of 37° C. and a rotation speed of 250 r / min;

[0043](3) Mix the strains obtained in step (2) according to the ratio, add them again to the culture medium prepared in step (1) to amplify together, and expand the culture for 48 hours at a temperature of 37°C and a rotation speed of 250r / min , to obtain the fermentation liquid;

[0044] (4) Mix the bran sterilized and cooled in advance according to the mass ratio of 30:1 to the fermented liquid, air-dry it na...

Embodiment 3

[0046] Example 3: The enrichment ability of lead in soil by different combinations of Indian mustard and microbial agents.

[0047] The experiment uses a plastic pot with a diameter of about 10cm, and each pot is filled with about 1kg of soil. After the soil is air-dried, it passes through a 2mm sieve. The heavy metal cadmium is added to the pot in the form of a solution and mixed evenly. The amount of water is 80% of the maximum water holding capacity in the field. After a week of natural balance, Indian mustard seeds are sprinkled in the pots, and the seeding rate is 4g / m 2 , the upper part is covered with soil 1cm, and after 10 days of plant growth, the experiment of each treatment group is started, and the total amount of the experimental group with microbial agent is 2Kg / m 2 .

[0048] in,

[0049] Group 1 consisted of Indian mustard only, without adding any microorganisms.

[0050] Group 2 was Indian mustard + Bacillus mycoides.

[0051] Group 3 is Indian mustard + S...

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Abstract

The invention provides a method for repairing lead-polluted soil by the combination of plants and microorganisms. The method comprises the following steps: uniformly scattering Indian leaf mustard seeds into lead-containing soil and watering to keep a moist soil condition; and after 10-15 days, adding a microorganism compound bacterial agent into the Indian leaf mustard root soil when the plants sprout and grow, so as to remove lead in the soil by the combination of the absorption effect on the lead of Indian leaf mustards and the strengthening effect of the microorganism compound bacterial agent, wherein the microorganism compound bacterial agent is prepared by mixing Bacillus mycoides, Shewanella and Pseudomonas aeruginosa in a mass ratio of (3-10) to 1 to 1. According to the method, the operation is convenient, the cost is low, the management is easy, secondary pollution cannot be caused, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of environmental restoration, and relates to a method for jointly repairing lead-contaminated soil with plants and microbial agents. Background technique [0002] Lead is a toxic heavy metal element that seriously affects human health and the survival of animals and plants. Lead pollution in industrial production, on the one hand, constitutes a pollution source through metal mining, smelting, and metal product production, polluting the surrounding environment with lead dust and smoke, and endangering the surrounding population. Lead-containing dust particles in the atmosphere can directly fall to the ground, or lead-containing waste residues are piled up on the ground, or lead-containing sewage and sludge are used as fertilizers, which can cause lead pollution to the soil. After lead accumulates in the topsoil, it will affect the soil biochemical activity, reduce the yield of crops, and affect human health ...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10C09K17/00C12N1/20C12R1/07C12R1/385C12R1/01
CPCC09K17/00C12N1/20B09C1/00B09C1/105
Inventor 谢婧婧宋天顺
Owner NANJING UNIV OF TECH
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