Shewanella and application thereof to control of soil nitrogen pollution in vegetable field

A technology of Shewanella and soil nitrogen, applied in the field of soil nitrogen pollution control, to achieve the effect of promoting plant growth, controlling soil nitrogen pollution, and reducing pollution problems

Active Publication Date: 2019-04-09
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the above-mentioned problem of soil nitrogen pollution and provides a Shewanella bacterium and its

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Isolation, cultivation, screening and purification of Shewanella A0B14

[0031] The sample is the rhizosphere soil in the greenhouse of Hongqiang Vegetable Production and Marketing Professional Cooperative in Lanling County, Shandong Province. The collected soil is put into a sealed bag and recorded.

[0032] After the soil sample was brought back to the laboratory, take 0.1g of the soil sample and add it to 5 mL of PBS buffer solution. -8 , then take 10 -4 to 10 -7 The diluted solution was spread on the LB plate, and after 36 hours of cultivation in the incubator at 28°C, a single colony was picked and streaked on a new LB plate, and the streaked single colony was picked and stored on the LBA plate.

[0033] Prepare reagents A and B, A is 5 mol / L of aminobenzenesulfonic acid (100 mL of acetic acid solution), B is 5 mol / L of α-naphthylamine (100 mL of acetic acid solution), and a part of the pure strain is inoculated in the nitrate medium (containing small inverted t...

Embodiment 2

[0038] Shewanella Shewanella Growth-promoting effect of sp. A0B14 on pepper

[0039] Use sterile soil to plant peppers in flowerpots, and conduct experiments when the plant height is about 7cm.

[0040] The preparation method of Shewanella A0B14 fermented liquid, concrete steps are as follows:

[0041] (1) Seed cultivation

[0042] Preparation of primary seeds: Use LB medium as the medium, with 10g of peptone, 10g of sodium chloride, 5g of yeast extract, 10g of agar, adjust the pH to 7.0-7.2, add water to 1000 mL, and sterilize at 121°C Standby for 15 minutes; the composition of the liquid medium LB is similar to that of the solid medium, except that agar is not added. After AOB14 was inoculated into 200ml of the above LB medium, cultured overnight at 180rpm / min at 30°C until OD 600 0.6-0.8, the above-mentioned culture solution is used as the primary seed.

[0043] Preparation of secondary seeds: The medium used is LB medium, the ingredients are peptone 10g, sodium chlori...

Embodiment 3

[0048] Shewanella Shewanella Control effect of sp. A0B14 soil nitrogen pollution control bacterial agent on vegetable soil nitrogen pollution

[0049] The preparation method of Shewanella A0B14 fermented liquid, concrete steps are as follows:

[0050] (1) Seed cultivation

[0051] Preparation of primary seeds: Use LB medium as the medium, with 10g of peptone, 10g of sodium chloride, 5g of yeast extract, 10g of agar, adjust the pH to 7.0-7.2, add water to 1000 mL, and sterilize at 121°C Standby for 15 minutes; the composition of the liquid medium LB is similar to that of the solid medium, except that agar is not added. After AOB14 was inoculated into 200ml of the above LB medium, cultured overnight at 180rpm / min at 30°C until OD 600 0.6-0.8, the above-mentioned culture solution is used as the primary seed.

[0052] Preparation of secondary seeds: The medium used is LB medium, the ingredients are peptone 10g, sodium chloride 10g, yeast extract 5g, agar 10g, pH is adjusted to...

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PUM

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Abstract

The invention relates to the technical field of control of soil nitrogen pollution, in particular to shewanella and application thereof to control of soil nitrogen pollution in a vegetable field. Theshewanella has the collection number of CGMCC No. 16843 and is collected on November 30, 2018. The invention further discloses molecular biological characteristics and a culture method of the strain.The shewanella is verified to have the characteristic of promoting crop growth, and is further used as a soil conditioner for controlling the soil nitrogen pollution in the vegetable field.

Description

technical field [0001] The invention relates to the technical field of soil nitrogen pollution control, in particular to a Shewanella bacterium and its application in controlling vegetable field soil nitrogen pollution. Background technique [0002] my country is a traditional agricultural country. In order to pursue high yields of vegetable crops, farmers often use a large amount of nitrogen fertilizer in vegetable fields. Vegetables have a short growth cycle, and the frequency of fertilization and irrigation is higher than that of other crops. For a long time, the nitrogen input into vegetable soil is far greater than the crop demand, which leads to the continuous accumulation of nitrogen content in the soil, especially the nitrate in vegetable soil. The nitrogen content increased sharply. Moreover, the accumulation of nitrogen leads to a decrease in the absorption and utilization of nitrogen by crops. At the same time, nitrate nitrogen is not easily absorbed by soil coll...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00B09C1/10C12R1/01
CPCA01N63/00B09C1/10C12N1/20C12N1/205C12R2001/01Y02P60/21
Inventor 周方园张新建谢雪迎吴晓青赵晓燕周红姿张广志范素素
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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