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Biomaterial for in situ repair of organic contamination soil, preparation method and application thereof

A technology of biomaterials and composite microbial agents, applied in the field of environmental engineering, to achieve significant synergistic effects, promote degradation, and increase soil organic matter content

Active Publication Date: 2019-05-31
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many technologies for preparing various remediation materials by using biochar, but there are few reports on in situ bioremediation of contaminated soil by using compost products and using humus respiration flora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of compound microbial agent

[0024] Pseudomonas putrefaciens (S.putrefaciens, such as China Microorganism Strain Inquiry Network, numbering bio-097659), Metallireducens (G.metallireducens, such as China Microbial Strain Inquiry Network, numbering bio-091064), seaweed Warnerella (S.alga, e.g. China Microorganism Strain Inquiry Network, No. bio-74287), thermophilic methanogenic archaea (M.thermolithotrophicus, e.g. China Agricultural Microorganism Culture Collection Management Center, No. ATCC51181), anaerobic ammonia Brocardia oxidans (B.anammoxidans, such as the research progress of metabolic pathways and key enzymes of anammox bacteria, Zhou Yingjie, Wang Shumei, Zhang Zhaoji, Chen Shaohua, "Journal of Ecology", 2012, 31(3 ); 738-744) and Bacillus subtilis (B.subtilis, such as China Microbial Strain Inquiry Network, No.: bio-103318) were separately cultured to OD after activation 600 0.4, then mix evenly according to the weight of the bacteria 2:3:1:2:...

Embodiment 2

[0032] 1. Preparation of compound microbial agent

[0033] Pseudomonas putrefaciens (S.putrefaciens), metallireducens (G.metallireducens), algal Shewanella (S.alga), thermophilic methanogenic archaea (M.thermolithotrophicus), anammox cloth After activation, B. anammoxidans and B. subtilis were cultured separately to OD 600 0.7, and then mix evenly according to the weight of the cells 1:4:2:2:2:5.

[0034] 2. Preparation of biochar

[0035] Wash the corncobs with water, dry them, crush them through a 100-mesh sieve, adjust the moisture content to 18%, and place them in a muffle furnace at 400°C for pyrolysis and carbonization for 10 hours.

[0036] 3. Preparation of humus

[0037] Mixed composting with agricultural waste and livestock manure or household waste as raw materials requires a humus content of 30%, a humic acid: fulvic acid content ratio range of 3:1, a moisture content of 20%, and heavy metal content indicators that must meet the national standards. Relevant sta...

Embodiment 3

[0041] 1. Preparation of compound microbial agent

[0042] Pseudomonas putrefaciens (S.putrefaciens), metallireducens (G.metallireducens), algal Shewanella (S.alga), thermophilic methanogenic archaea (M.thermolithotrophicus), anammox cloth After activation, B. anammoxidans and B. subtilis were cultured separately to OD 600 0.5, then mix evenly according to the weight of the cells 2:4:2:2:2:4.

[0043] 2. Preparation of biochar

[0044] Wash the peanut shells with water, dry them, crush them through a 100-mesh sieve, adjust the moisture content to 20%, and place them in a muffle furnace at 450°C for 9 hours of pyrolysis and carbonization.

[0045] 3. Preparation of humus

[0046] Mixed composting with agricultural waste and livestock manure or household waste as raw materials requires a product with a humus content of 40%, a humic acid: fulvic acid content ratio of 6:1, a moisture content of 30%, and heavy metal content. Requirements of relevant national standards.

[0047...

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PUM

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Abstract

The invention relates to a composite microbial agent. The agent is characterized by being prepared from the following microorganisms of Shewanella putrefaciens, Geobacter metallireducens, Shewanella alga,Methanococcus thermolithotrophicus,Brocadia anammoxidans and Bacillus subtilis, preferably, the mass raatio of the Shewanella putrefaciens to the Geobacter metallireducens to the Shewanella alga to the Methanococcus thermolithotrophicus to the Brocadia anammoxidans to the Bacillus subtilis is A:B:C:D:E:F, A is selected from the numbers of 1-3,B is selected from the numbers of 3-5, C is selected from the numbers 1-3, D is selected from the numbers of 2-4, E is selected from the numbers of 1-3, and F is selected from the numbers of 3-5; a preparation method and application thereof are also provided. The microbial agent has very excellent effects on repairing organic mattercontamination, and has a simple preparation method, low cost, no secondary pollutionand a broad market value.

Description

technical field [0001] The invention belongs to the field of environmental engineering, and in particular relates to a biological material for in-situ repairing organic polluted soil and improving soil quality and a preparation method thereof. Background technique [0002] Soil pollution is an important global environmental problem. The remediation of soil pollution and its mechanism have always been a hot spot of continuous research in many fields. Organic polluted soil is carcinogenic, hysteresis, long-term and irreversible, making it difficult to remediate. In recent years, with the rapid development of my country's industrialization and urbanization, organic pollutants in soil have been detected from time to time, which has attracted great attention from the country. Compost products are not only high in humus, but also rich in nutrients, and are often used as organic fertilizers to apply to soil. Therefore, the direct use of compost products in soil remediation not on...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/08
Inventor 黄彩红席北斗唐朱睿夏湘勤刘可欣李一葳
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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