A strain of Brevibacillus lateralsporosa with rapid degradation of nitrite nitrogen and antibacterial function and its application

A Brevibacillus brevis, nitrite nitrogen technology, applied in the field of microorganisms, can solve the problems of unsuitable marine aquaculture, less research, etc., and achieve the effect of overcoming the frequent occurrence of epidemic diseases and the abuse of antibiotics and having broad application prospects.

Active Publication Date: 2017-08-22
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these bacillus are freshwater strains, which are not suitable for mariculture. There are few studies on bacillus and their microbial preparations that are specially used for mariculture and have high-efficiency purification of mariculture water.

Method used

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  • A strain of Brevibacillus lateralsporosa with rapid degradation of nitrite nitrogen and antibacterial function and its application
  • A strain of Brevibacillus lateralsporosa with rapid degradation of nitrite nitrogen and antibacterial function and its application
  • A strain of Brevibacillus lateralsporosa with rapid degradation of nitrite nitrogen and antibacterial function and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Screening, isolation and identification of embodiment 1 bacterial strain

[0018] 1. Preparation of culture medium:

[0019] Nitrite nitrogen degrading bacteria enrichment screening liquid medium: NaNO 2 1g, Na 2 CO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.03g, FeSO 4 ·7H 2 O 0.4g, aged sea water 1L, pH 7.2-7.5.

[0020] Solid medium for isolation and purification of nitrite nitrogen degrading bacteria: NaNO 2 0.5g, Na 2 CO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.03g, FeSO 4 ·7H 2 O 0.4g, aged sea water 1L, agar powder 12g, pH 7.2~7.5.

[0021] 2216E liquid medium: peptone 5g, yeast extract 1g, FePO 4 0.1g, old sea water 1L, pH 7.2; solid medium is liquid medium by adding 15g l -1 agar powder.

[0022] Nutrient broth (NB) culture medium: peptone 10g, beef extract powder 3g, sodium chloride 30g, distilled water 1000ml, pH value 7.2; solid culture medium is liquid culture medium by adding 15g l -1 agar powder.

[0023] Before use, the above medium was ...

Embodiment 2

[0030] Morphological characteristics and physiological and biochemical characteristics of embodiment 2 YB-02

[0031] Morphological characteristics of YB-02: such as figure 1 As shown, the colony of YB-02 on the 2216E plate is round, earthy yellow, smooth, moist and shiny, slightly raised in the middle, opaque, with neat edges and no halos.

[0032] Table 1. Culture characteristics of strains on 2216E medium

[0033]

[0034] According to the physiological and biochemical detection method of "Bergery Bacteria Identification Manual" (eighth edition), the Brevibacillus spp. YB-02 was detected.

[0035] The isolated and purified YB-02 has wide salinity, can grow in the salinity range of 20-40‰, and the optimum growth salinity is in the range of 28-32‰; it can grow normally in the temperature range of 10-40℃, and the most suitable The growth temperature is 20-30°C; it can grow in the range of pH 7-9, the optimum growth pH is 7.2-8.5, and it has poor tolerance to acidic environm...

Embodiment 3

[0040] Growth characteristics and culture conditions of embodiment 3 Brevibacillus lateralsporosa YB-02

[0041] Brevibacillus laterosporu (Brevibacillus laterosporu) CGMCC-No.9757 has eurysalinity because it is isolated from the marine aquaculture environment, and the optimum growth salinity is 28-32‰; the optimum growth temperature is 26-28°C, The growth pH is 7.2-8.5. After 14-16 hours of fermentation, the logarithmic phase is reached, and the number of bacteria reaches 5×10 10 cfu / mL.

[0042] The medium used for cultivation is as follows:

[0043] Seed medium (nutrient broth (NB) medium): beef extract powder 5g, peptone 10g, sodium chloride 30g, water 1L, pH7.2-7.5.

[0044] 2216E liquid medium: peptone 5g, yeast extract 1g, FePO4 0.1g, aged sea water 1L, pH 7.2~7.5.

[0045] Fermentation medium: 150g of corn flour, 250g of bean cake powder, 3.5g of medium-temperature amylase, 2.5g of neutral protease, 40g of corn steep liquor, 25g of glucose, 10g of magnesium sulfate...

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Abstract

The invention discloses a strain of Brevibacillus laterosporu with the function of rapidly degrading nitrite nitrogen and antibacterial function and its application. The screened strain is Brevibacillus laterosporu (Brevibacillus laterosporu) YB-02, and its strain preservation number is CGMCC-No. 9757. The bacterial strain of the present invention has the ability to efficiently degrade nitrite nitrogen in water body, bile salt resistance and trypsin resistance, and also has high inhibition to mariculture pathogenic bacteria such as Vibrio splendidus, Shewanella yellow sea and Edwardsiella tarda Effect. Therefore, the bacterial strain of the present invention can be developed as a probiotic product for water purification and bacteriostasis in marine aquaculture, avoiding the abuse of antibiotics, effectively preventing the occurrence of fishery diseases, improving the quality and output of seafood cultivation, and having high efficiency, fast and safe for acute water pollution. The water purification effect will have broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a strain of Brevibacillus lateralsporosa with the function of rapidly degrading nitrite nitrogen and the function of inhibiting bacteria and its application. Background technique [0002] Seawater is the basic environmental condition for the survival of seawater animals. As the saying goes: "Three points for fish, seven points for water". The quality of water quality directly affects their growth status and the economic benefits of farmers. With the improvement of living standards, people's demand for aquatic products is increasing day by day. When the catch cannot meet the market demand, high-density intensive aquaculture has developed rapidly. However, the degree of pollution of the water body in this aquaculture model far exceeds the purification ability of the water body itself. The residual bait and excrement are deposited in the water body, causing the water body itse...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C02F2101/166C12N1/205C12R2001/01
Inventor 徐永平车鉴李淑英李晓宇王丽丽王熙涛张建成阳桂菊
Owner DALIAN UNIV OF TECH
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