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Bacillus capable of resisting various aquaculture pathogenic bacteria and application thereof

A technology of pathogenic bacteria and mariculture, applied in the direction of application, bacteria, and microbial-based methods, can solve problems such as drug residues, aggravating animal diseases, and water environment pollution

Active Publication Date: 2018-04-27
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the prevention and control methods for bacterial aquatic diseases in my country still mainly rely on chemical drugs such as antibiotics. Antibiotic treatment and other measures can achieve the purpose of partially controlling the occurrence of diseases and promoting the growth of farmed animals, but the resulting drug resistance of pathogenic microorganisms and drug residues , water environment pollution and aquatic product quality and safety are becoming more and more serious; at the same time, due to the long-term use of these drugs, while killing pathogenic organisms, they also damage the normal microbial barriers of the skin and intestinal tract of farmed animals, making Those endogenous or exogenous pathogenic bacteria that were originally inhibited by the normal flora barrier can reproduce in large numbers, destroying the micro-ecological balance of the normal microbiota in the epidermis and intestinal tract of farmed animals, making the incidence of farmed animal diseases worse

Method used

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  • Bacillus capable of resisting various aquaculture pathogenic bacteria and application thereof
  • Bacillus capable of resisting various aquaculture pathogenic bacteria and application thereof

Examples

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Effect test

Embodiment 1

[0013] Example 1 Isolation, Identification and Preservation of Bacillus amyloliquefaciens InAD-160

[0014] Step 1. Strain separation and purification: The original sample collection place of the bacterial strain InAD-160 obtained in the present invention is the bottom seawater of a station (E88°; S4°) in the Indian Ocean. After the sample is collected, take 1 ml of seawater sample and add sterile glycerol to a final concentration of 20%, immediately store it in a -80°C refrigerator, take it back to the laboratory, take out the sample and thaw and aseptically apply it to MA 2216E medium (Difco, Cat. No. : 212185), put the coated petri dish upside down at 25°C and incubate for 24-96 hours, pick out the single colony that grows, line it on the MA2216E medium plate, and turn the marked petri dish upside down Cultivate at 25°C for 24-96 hours, pick out the single colony that grows, and line it again on the MA 2216E medium plate for purification, place the marked Petri dish upside ...

Embodiment 2

[0028] Example 2. Application of Bacillus amyloliquefaciens InAD-160

[0029] Step 1. Preparation of Bacillus amyloliquefaciens InAD-160 bacterial solution: Streak the strains stored at -80°C on 2216E seawater solid medium, culture at 25°C-30°C, pick a single colony and inoculate it in fresh 2216E seawater The liquid medium was fermented and cultivated at 25-30°C and 120-200rpm for 48 hours to obtain a fermentation broth, and the strain density of the fermentation broth was 1×10 9 / ml.

[0030]Step 2. Preparation of bait: The basic bait used in this experiment is the bait of juvenile fish from Santong Bioengineering (Weifang) Co., Ltd. The bait of the experimental group was prepared according to the standard of mixing 4ml of fermentation broth per 10g of basic bait, and the bait of the control group was mixed with cultured seawater.

[0031] Step 3. Turbot culture: 180 healthy turbots with an average body length of 8.37±1.13cm and an average weight of 9.2±1.99g were temporar...

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Abstract

The invention discloses a bacterial strain capable of resisting various aquaculture pathogenic bacteria and application thereof. The antagonistic bacterium is Bacillus amyloliquefaciens InAD-160, andthe microbial collection number is CGMCC No.14030. The Bacillus amyloliquefaciens InAD-160 is obtained by separating deep seawater of the Indian Ocean, can inhibit growth of various aquaculture pathogenic bacteria like Edwardsiella tarda, Aeromonas hydrophila, Shewanella putrefaciens and Vibrio vulnificus and can be used for preventing and controlling aquaculture diseases caused by the same. The Bacillus amyloliquefaciens InAD-160 obtained from deep sea is a biological prevention and control potential strain which is high in prevention and control efficiency and wide in prevention and controlrange and has great application and development prospect.

Description

technical field [0001] The invention belongs to the technical field of beneficial bacteria screening, and in particular relates to a bacillus capable of antagonizing various aquaculture pathogenic bacteria and an application thereof. Background technique [0002] my country is a big aquaculture country in the world, and it is the only country in the world where aquaculture production exceeds fishing production. However, in my country, aquaculture is affected to varying degrees by aquaculture diseases every year, whether in coastal or inland areas, causing huge losses and harm to the aquaculture industry. Bacterial diseases are relatively more common in aquaculture diseases, and the prevalence is relatively wide. For example, Edwardsiella tarda of the Enterobacteriaceae family, Edwardsiella genus, is a Gram-negative enteric pathogen that has caused diseases in more than 20 kinds of fish, such as eel, flounder, tilapia, Chinese soft-shelled turtle, Carp, etc. have caused hug...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/80C12R1/07
CPCA23K10/18A23K50/80C12N1/205C12R2001/07
Inventor 曲凌云尚琨杜光迅高萍
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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