Low-temperature-resistant composite microbial agent used for treating contaminated water, and preparation method and application thereof

A compound microbial inoculum, low temperature resistant technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of reduced enzyme efficiency, difficult to reach standards, enzyme denaturation inactivation, etc., to reduce the COD of sewage. and ammonia nitrogen content, prevent secondary pollution, and have the effects of strong adaptability

Inactive Publication Date: 2016-02-03
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Enzymes in organisms are greatly affected by temperature. Too high a temperature will denature and inactivate the enzyme, and too low a temperature will reduce the efficiency of the enzyme or even dormant. reduce
Therefore, under low temperature conditions, the biological treatment of urban sewage has long had the problem of poor treatment effect and difficult treatment standards.
In northern China, the seasons are distinct, the winter is long, and the temperature often cannot meet the requirements of biological treatment. Therefore, the problem of sewage treatment in winter has always been a major problem for people.
[0004] The metabolism of microorganisms can absorb various pollutants (mainly organic matter) in polluted water, because each microorganism plays a different role in the circulation of water substances; it is difficult for a single strain to meet the biological treatment of urban sewage, especially Requirements for handling at low temperatures

Method used

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  • Low-temperature-resistant composite microbial agent used for treating contaminated water, and preparation method and application thereof
  • Low-temperature-resistant composite microbial agent used for treating contaminated water, and preparation method and application thereof
  • Low-temperature-resistant composite microbial agent used for treating contaminated water, and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0034] A method for preparing a low-temperature-resistant composite microbial bacterial agent for treating polluted water, comprising the following steps:

[0035] (1) Cultivation of Shewanella putrefaciens: At room temperature, take Shewanella putrefaciens from -80°C, draw 200 μl, and evenly spread it on the sterilized solid sewage medium with a coating stick, seal the film Seal tightly, place the plate upside down in a 20°C incubator and cultivate for 12 hours, pick a large and smooth single colony, inoculate it into the sterilized liquid sewage medium, and culture it in a shaker at 20°C at a speed of 200rpm. About 12 hours, detect the number of live bacteria of Shewanella putrefaction, and the number of bacteria is greater than or equal to 3×10 8 pcs / ml bacterial liquid;

[0036] (2) Cultivation of Pseudomonas testosterone: At room temperature, take out Pseudomonas testosterone from -80°C, draw 200 μl, and evenly spread it on the sterilized solid sewage medium with a coati...

Embodiment 2

[0039]A method for preparing a low-temperature-resistant composite microbial bacterial agent for treating polluted water, comprising the following steps:

[0040] (1) Cultivation of Shewanella putrefaciens: At room temperature, take Shewanella putrefaciens from -80°C, draw 200 μl, and evenly spread it on the sterilized solid sewage medium with a coating stick, seal the film Seal tightly, place the plate upside down in a 15°C incubator and cultivate for 14 hours, pick a large and smooth single colony, inoculate it into the sterilized liquid sewage medium, and culture it in a shaker at 15°C at a speed of 150rpm. About 12 hours, detect the number of live bacteria of Shewanella putrefaction, and the number of bacteria is greater than or equal to 1×10 8 pcs / ml bacterial liquid;

[0041] (2) Cultivation of Pseudomonas testosterone: At room temperature, take out Pseudomonas testosterone from -80°C, draw 200 μl, and evenly spread it on the sterilized solid sewage medium with a coatin...

Embodiment 3

[0044] A method for preparing a low-temperature-resistant composite microbial bacterial agent for treating polluted water, comprising the following steps:

[0045] (1) Cultivation of Shewanella putrefaciens: At room temperature, take Shewanella putrefaciens from -80°C, draw 200 μl, and evenly spread it on the sterilized solid sewage medium with a coating stick, seal the film Seal tightly, place the plate upside down in a 25°C incubator and cultivate it for 10 hours, pick a large and smooth single colony, inoculate it into the sterilized liquid sewage medium, and culture it in a shaker at 25°C at a speed of 250rpm. About 12 hours, detect the number of live bacteria of Shewanella putrefaction, and the number of bacteria is greater than or equal to 5×10 8 pcs / ml bacterial liquid;

[0046] (2) Cultivation of Pseudomonas testosterone: At room temperature, take out Pseudomonas testosterone from -80°C, draw 200 μl, and evenly spread it on the sterilized solid sewage medium with a co...

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Abstract

The invention discloses a low-temperature-resistant composite microbial agent used for treating contaminated water, and a preparation method and application thereof. The microbial agent is composed of Shewanella putrefaciens liquid and Pesudomonas testosterone liquid in a volume ratio of (1-2): (1-2). The low-temperature-resistant composite microbial agent has strong adaptability to environments, particularly has low temperature resistance and can normally grow and metabolize in a low-temperature environment. Environment-friendly microbes are employed to strengthen the self-cleaning function of a water body, digest pollutants in river water and organic matters in sludge at river bottom and to prevent secondary pollution of the water body. In use, the composite microbial agent is directly added without complex engineering process, so the composite microbial agent is convenient to use. The composite microbial agent can effectively reduce the contents of COD and ammonia nitrogen in sewage under a low temperature condition, and removal efficiency of the composite microbial agent is obviously better than removal efficiency of a single microbial strain.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a low-temperature-resistant composite microbial bacterial agent, its preparation method and application. Background technique [0002] River water pollution has become the main factor affecting regional socio-economic development and restricting the improvement of people's living standards. Although methods such as reducing sludge load and increasing sludge concentration have improved the treatment effect to a certain extent, the operating efficiency and operating cost are not ideal, and the long-term polluted river water still cannot be improved. There are self-purifying microorganisms in rivers with better ecology and less pollution, but the self-purifying function has been lost in polluted rivers. Utilize and further use for environmental governance are very few. [0003] In recent years, microbial agents have been used in sewage treatment, but there are many proble...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
Inventor 季静史利平王罡金超尹日晖
Owner TIANJIN UNIV
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