Chryseobacterium sp. And application thereof in promoting corn growth and iron absorption

Abstract

The invention relates to a strain of Chryseobacterium sp. ZJUHULYY1 and application of the Chryseobacterium sp. ZJUHULYY1 in promotion of corn growth and iron absorption, and provides a strain of Chryseobacterium sp. ZJUHULYY1, which is preserved in China Center for Type Culture Collection on April 27, 2025, and has a preservation number of CCTCC NO: M 2025904. The strain of Chryseobacterium sp. ZJUHULYY1 is obtained by separating and purifying from rhizosphere soil of corn, and the strain of Chryseobacterium sp. ZJUHULYY1 is obtained by separating and purifying from rhizosphere soil of corn. The biological characteristics of the strain are as follows: after being cultured in an LB plate culture medium at 30 DEG C for 24 hours, the bacterial colony is round or oval, golden yellow, smooth and glossy in surface, neat in edge and negative in gram staining; when the strain is inoculated to rhizosphere of corn growing in calcareous soil, compared with a control group, the plant height, chlorophyll content, biomass and active iron content of the corn are all remarkably improved, the growth condition of the corn in the calcareous soil is effectively improved, and the absorption and utilization efficiency of the corn on iron elements is improved.

Description

Technical Field

[0001] The invention relates to the technical field of soil microorganisms, in particular to a Chryseobacterium strain and application thereof in promoting corn growth and iron absorption. Background Art

[0002] Iron is a cofactor for many enzymes involved in electron and oxygen transfer processes and is involved in cellular metabolic reactions such as photosynthesis and respiration. Although iron is abundant in most soils, its solubility at neutral and alkaline pH levels is extremely low, so it primarily exists as insoluble compounds such as iron hydroxide and iron oxide. This insoluble iron is difficult for plant roots to directly absorb and utilize, resulting in chronically low iron bioavailability. This severely impacts iron absorption in crops like corn, limiting their growth and development.

[0003] Currently, agricultural production primarily relies on the application of iron fertilizers for iron supplementation. While this can directly replenish iron in the soil, it is susceptible to soil conditions, such as pH and organic matter content, resulting in low iron fertilizer utilization efficiency. Furthermore, long-term application of iron fertilizers can lead to a series of environmental problems, such as soil structure damage and imbalances in soil pH, negatively impacting the sustainability of soil ecosystems.

[0004] There is no research and application of Chryseobacterium in promoting corn growth and iron absorption in agricultural production. It has been reported that Chryseobacterium has significant effects in applications such as degrading chlorpyrifos, promoting the growth of yam tissue culture seedlings, and degrading seaweed residue. For example, CN115637238A discloses a strain of Chryseobacterium and its application. The Chryseobacterium has the characteristics of promoting growth and degrading chlorpyrifos. It can be prepared into a microbial agent, which can enhance the degradation of residual chlorpyrifos in rice and promote rice growth. It is used to repair chlorpyrifos residual pollution in rice planting systems. CN116676215A discloses a strain of Chryseobacterium and its application in promoting the growth of yam tissue culture seedlings. The strain can significantly promote the growth of yam tissue culture seedlings. CN114717145B discloses a strain of Chryseobacterium cucumber and its application in degrading seaweed residue. The Chryseobacterium can not only efficiently degrade seaweed residue, but also contains ACC deaminase that can degrade ethylene synthesis precursors. This strain can not only provide plants with the nutrients they need for growth by transforming and degrading seaweed residue, but can also enhance plant tolerance and promote plant growth under adverse conditions.

[0005] Chryseobacterium is widely present in nature. Existing studies have shown that Chryseobacterium plays a role in degrading chlorpyrifos, promoting the growth of yam tissue culture seedlings, and degrading seaweed residue. However, there are no reports on Chryseobacterium's role in promoting corn growth and iron absorption. Summary of the Invention

[0006] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide a strain of Chryseobacterium and its application in promoting corn growth and iron absorption, so as to solve the problem in the background technology that corn and other crops have low absorption and utilization efficiency of iron elements, thereby limiting the growth and development of corn.

[0007] In order to achieve the above object, the present invention proposes a Chryseobacterium ( Chryseobacterium sp .ZJUHULYY1), which was deposited in the China Center for Type Culture Collection on April 27, 2025, with the deposit number being CCTCC NO: M 2025904.

[0008] The Chryseobacterium Chryseobacterium sp The .ZJUHULYY1) strain was isolated and purified from the rhizosphere soil of corn.

[0009] The biological characteristics of the strain are as follows: after culturing in LB plate medium at 30°C for 24 hours, the colonies are round or oval, golden yellow in color, smooth and shiny on the surface, with neat edges, and Gram staining is negative.

[0010] The present invention provides Chryseobacterium ( Chryseobacterium sp .ZJUHULYY1), which promotes maize growth and iron absorption, has shown significant effectiveness in applications that promote maize growth and iron absorption. Experimental results showed that when this strain was inoculated into the rhizosphere of maize grown in calcareous soil, plant height, chlorophyll content, biomass, and active iron content were significantly increased compared to the control group. This effectively improved maize growth in calcareous soil and increased maize's iron absorption and utilization efficiency, suggesting broad application prospects in agricultural production.

[0011] Compared with the prior art, the present invention has the following beneficial effects: The experimental results showed that the Chryseobacterium sp When the strain ZJUHULYY1 (.ZJUHULYY1) was inoculated into the rhizosphere of maize grown in calcareous soil, plant height, chlorophyll content, biomass, and active iron content were significantly increased compared to the control group. This effectively improved maize growth in calcareous soil and increased its iron absorption and utilization efficiency, demonstrating broad application prospects in agricultural production. It provides an efficient and environmentally friendly solution for maize growth and iron absorption, increasing maize yield and quality while reducing environmental pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0013] Figure 1 Figure 3 is a colony morphology diagram in the LB culture medium of the present invention.

[0014] Figure 2 This is a graph showing the experimental results of the present invention's Chryseobacterium promoting corn plant height and chlorophyll content.

[0015] Figure 3 This is a diagram showing the experimental results of the present invention's Chryseobacterium promoting the accumulation of corn biomass.

[0016] Figure 4 This is a diagram showing the experimental results of the present invention using Chryseobacterium chryseum to increase the active iron content in corn.

[0017] Figure 5 This is a phenotypic diagram of the Chryseobacterium spp. promoting corn growth and iron absorption. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. The components of the embodiments of the present invention generally described and shown in the drawings herein can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0019] Example 1: Chryseobacterium Chryseobacterium sp. ZJUHULYY1) was isolated and identified.

[0020] In the rhizosphere soil of corn, the strains of Chryseobacterium (such as Figure 1 Its colonies are round or oval, golden yellow in color, smooth and shiny, with neat edges, and Gram-negative.

[0021] In the clean bench, a single colony was picked using a sterile inoculation needle and placed in a PCR tube containing 20 μL of sterile water. The suspension was lysed at 98°C for 5 minutes. The bacterial suspension was then diluted to 100 μL as a DNA template. 16S rRNA sequence amplification was performed using primers 27F and 1492R. The PCR product was electrophoresed on a 1% agarose gel. Sequencing analysis showed that the 16S rRNA sequence amplified by the strain was 1388 bp long. BLAST comparison analysis of the 16S rRNA sequence of the strain showed that the strain was similar to the Chryseobacterium bernardetii The highest similarity is 99.06%, which may be a closely related species.

[0022] The results of 16S rDNA gene sequencing of the strain are as follows: Example 2: Chryseobacterium Chryseobacterium sp. Preparation of ZJUHULYY1 bacterial solution.

[0023] The strain was inoculated into LB liquid medium and cultured in a shaker at 30°C and 180 rpm for 24 h to obtain a suspension of Chryseobacterium. The suspension was centrifuged at 8500 rpm for 5 minutes, the cells were collected, washed twice with sterile water, and the OD was adjusted. 600 =1, that is, the bacterial solution of Chryseobacterium chryseum is obtained.

[0024] Example 3: Chryseobacterium Chryseobacterium sp. ZJUHULYY1) promotes corn growth and iron absorption.

[0025] Calcareous soil was collected for the greenhouse pot experiment. Each pot was filled with about 350 g of calcareous soil. About 7 days after corn was planted (two to three leaves), the Chryseobacterium ( Chryseobacterium sp. ZJUHULYY1) bacteria solution was evenly inoculated into the rhizosphere of corn, 15 ml per pot. Figures 2 - 5 As shown), after Chryseobacterium spp. ( Chryseobacterium sp. The corn treated with ZJUHULYY1) was superior to the control group in terms of plant height, chlorophyll content, biomass and active iron content, proving that the Chryseobacterium provided by the present invention ( Chryseobacterium sp. ZJUHULYY1) strain has a significant effect on promoting corn growth and iron absorption in calcareous soil.

[0026] Of course, the above description is only a specific embodiment of the present invention and is not intended to limit the scope of implementation of the present invention. Any equivalent changes or modifications made based on the structure, features and principles described in the scope of the patent application of the present invention should be included in the scope of the patent application of the present invention.

[0027] The embodiments described above are only specific implementation methods of the present invention, which are used to illustrate the technical solutions of the present invention rather than to limit them. The scope of protection of the present invention is not limited thereto. Although the present invention has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that any technician familiar with this technical field can still modify the technical solutions recorded in the aforementioned embodiments within the technical scope disclosed by the present invention, or make equivalent replacements for some of the technical features therein; and these modifications, changes or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention, and should all be covered by the scope of protection of the present invention.

Claims

1. A strain of Chryseobacterium Chryseobacterium sp. ZJUHULYY1), which was deposited in the China Center for Type Culture Collection on April 27, 2025, with the deposit number being CCTCC NO: M 2025904.

2. A strain of Chryseobacterium according to claim 1 ( Chryseobacterium sp. ZJUHULYY1), characterized in that The Chryseobacterium strain is isolated and purified from corn rhizosphere soil.

3. A strain of Chryseobacterium according to claim 2 ( Chryseobacterium sp. ZJUHULYY1), characterized in that The biological characteristics of the strain are as follows: after culturing in LB plate medium at 30°C for 24 hours, the colonies are round or oval, golden yellow in color, smooth and shiny on the surface, with neat edges, and Gram staining is negative.

4. A strain of Chryseobacterium according to claim 1, 2 or 3 ( Chryseobacterium sp. ZJUHULYY1) in promoting corn growth and iron absorption.

5. The use according to claim 4, characterized in that Chryseobacterium Chryseobacterium sp. The application of ZJUHULYY1) in promoting corn growth and iron absorption refers to: inoculating the strain into the rhizosphere of corn grown in calcareous soil to improve the growth of corn in calcareous soil and enhance the efficiency of corn in absorbing and utilizing iron.

Images