Preparation method of bacteriocin-producing Lactobacillus plantarum subsp. Plantarum Zhang-LL active bacterial preparation

Abstract

The invention relates to a preparation method of a bacteriocin-producing Lactobacillus plantarum subsp. Plantarum Zhang-LL active bacterial preparation, and belongs to the field of microbe application. In the preparation method, by using a bacteriocin-producing Lactobacillus plantarum subsp. Plantarum Zhang-LL CGMCC No. 6936 bacterial strain, the active bacterial preparation is prepared through activation, expanding culture, high-density fermentation, centrifugation, freeze-drying protective agent addition, a prefreezing process, a freeze-drying process and other processes. The preparation method comprises the following steps: firstly, rapeseed peptone is selected as a substituting nitrogen source, so that the culture condition of the lactobacillus plantarum is optimized while the cost of a culture medium is reduced; a 5L of full-automatic fermentation tank is used for performing the high-density fermentation, and under the optimized low-cost culture medium and the optimized culture condition, the viable count of fermented liquid can reach above 1010CFU / mL. In the preparation method, potato pulp is used as a matrix of a freeze-drying protective agent, and an optimal combined formula of the freeze-drying protective agent is determined, so that the survival rate of the Lactobacillus plantarum subsp. Plantarum Zhang-LL reaches above 60% and the viable content can reach above 1011CFU / g. The prepared active bacterial preparation is low in raw material price and relatively high in viable content, can provide a good foundation for developing a novel feed additive for poultry, and can provide a significant economic value.

Description

technical field [0001] The invention relates to a method for preparing live lactic acid bacteria preparations in the technical field of microorganisms, belongs to the field of microorganism applications, and can be used in the production of microecological preparations such as live feed preparations in poultry and animal husbandry. Background technique [0002] Lactic acid bacteria are a type of probiotic bacteria that exist in the human body. Lactic acid bacteria are able to ferment carbohydrates into lactic acid, hence the name. Adding probiotic lactic acid bacteria to food can not only improve the nutritional value of food, but also have health effects, such as promoting the absorption of nutrients such as protein, producing a large number of beneficial substances such as vitamin B, improving the function of the human intestinal tract, and restoring the intestinal flora of the human body Balance, enhance human immunity, anti-tumor, anti-aging, and also have the effects o...

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Problems solved by technology

However, there are no relevant reports at home and abroad on the method of using bacteriocin-producing Lactobacillus plantarum subsp.

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