Fluorescence immunity chromatography test strip for escherichia coli O157:H7 detection

A technique of fluorescence immunochromatography and O157, which is applied in the detection field to achieve the effects of fast detection speed, high sensitivity and strong specificity
CN103376320AInactive Publication Date: 2013-10-30HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2013-10-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a fluorescence immunity chromatography test strip for escherichia coli O157:H7 detection. The test strip is prepared by a method comprising the following steps: 1) preparing water-soluble CdTe / CdS quantum dots with a core-shell structure; 2) marking escherichia coli O157:H7 rabbit polyclonal antibodies with the CdTe / CdS quantum dots; and 3) coating the escherichia coli O157:H7 marked rabbit polyclonal antibodies on a glass cellulose membrane to form a combination pad; respectively coating the escherichia coli O157:H7 polyclonal antibodies and goat anti-rabbit IgG on a cellulose nitrate membrane to form a detection line and a quality control line; and assembling a sample pad, the combination pad, the cellulose nitrate membrane and a piece of absorbent paper on a pvc base plate to form the fluorescence immunity chromatography test strip. The fluorescence immunity chromatography test strip has advantages of fast detection, high sensitivity, strong singularity and good stability in escherichia coli O157:H7 detection.
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Description

technical field

[0001] The invention belongs to the technical field of detection, and relates to a fluorescent immunochromatographic test strip for detecting Escherichia coli O157:H7 and an application thereof. Background technique

[0002] Escherichia coli O157:H7 is an enteropathogenic bacterium that infects humans mainly through ingestion of contaminated food or water, or contact with carrier animals, and can cause death in severe cases. Escherichia coli O157:H7 infection has gradually become an important public health problem threatening human health, and the establishment of rapid and sensitive detection methods is the key to the prevention and control of Escherichia coli O157:H7 infection.

[0003] Quantum dots are a new type of nanomaterial with excellent fluorescence properties. Compared with other immunological analysis methods, the quantum dot-based immunoassay method has great advantages in specificity, sensitivity and accuracy, and has broad application prospect...

Claims

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