95 results about "Growth-promoting factors" patented technology

The invention relates to a composite probiotic bacterium microecological preparation and a preparation method thereof. The preparation is prepared by mixing 1.0 to 20 percent of composite microbial bacterium powder, 5.0 to 50 percent of composite oligosaccharide, 30 to 80 percent of auxiliary protective carrier and 0.1 to 0.2 percent of edible essence. The composite probiotic bacterium microecological preparation is prepared from a beneficial microbial preparation and a microbial growth-promoting factor, namely the oligosaccharide, through special combination and technological recombination. The composite probiotic bacterium microecological preparation has the advantages that beneficial active bacteria and the oligosaccharide promote each other, so that the composite probiotic bacterium microecological preparation has the advantages of obviously improving the microecological environment of the whole intestine and contributing to the digestion, preventing and treating intestine diseases, treating diarrhea, chronic diarrhea, diarrhea which can not be cured by antibiotic, constipation, dyspepsia and abdominal distension which are caused by intestine dysbacteriosis, recovering the intestine flora balance, protecting the liver, reinforcing the functions of detoxication and toxin expulsion of the liver, reinforcing the disease prevention and resistance such as the resistance of human body, and the like.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.

The invention relates to a feed additive, in particular to a prepration method of a compound microbial feed additive, which is used for solving the problems that a variety of feed additives have respective different shortcomings in the actual applications. The preparation method comprises the following steps: preparing selenium yeast, iron yeast and zinc yeast which are rich in trace elements, taking vinegar residue, wheat bran, corn flour, compound Daqu, ammonium sulfate and other auxiliary materials as culture media, adding aspergillus niger, aspergillus oryzae, bacillus subtilis, lactobacillus and yeast, producing a multi-bacterial compound enzyme by the solid-state method, and mixing the functional yeast, phytase and the multi-bacterial compound enzyme for producing a biological feed micro-multi-enzyme which has a large number of live bacteria in beneficial bacterial groups, a complete enzyme system of digestive enzymes and non-digestive enzymes needed by animals and high enzyme activity, and is rich in organic trace elements, vitamins, amino acids and unknown growth-promoting factors with functional nutrition for the animals and people; furthermore, the feed additive has the advantages of advanced process, abundant nutrition, abundant bacterial groups, full enzyme spectrum, high activity, greenness, no pollution and the like.

The invention discloses a feed additive and feed containing the same. The additive is prepared by mixing a microbial ecological agent prepared from three probiotics, namely saccharomycetes, bacillus subtilis and aspergillus oryzae, with powder of various Chinese herbal medicines, and growth promoting factors such as chromium picolinate, zinc sulfate and sodium selenite vitamin E. The probiotics, powder of Chinese herbal medicines and growth promoting factors are organically and closely combined to produce an excellent synergetic effect, so that feed additive can prevent and control diseases in animals, promote the growth of the animals, improve the quality of livestock products and the like. The preparation process of the feed additive is simple, requires small investment and energy and avoids waste liquid pollution. The feed additive is proved by clinic animal feeding tests to have very obvious effect, is very popular among raisers and has a bright market prospect.

The invention relates to the microbiological engineering technology. Crop byproducts, such as agricultural straws, hulls and cakes and the like are utilized to produce high protein feed and animal promoters by multiple strains to lead wastes to be effectively utilized; waste materials are changed into valuable things, so that raw materials are inexhaustible, thus improving input-output ratio of feed with significant economic and social benefits. As containing a great number of active enzymes and growth promoting factors, the technology improves the function of animal metabolism, and leads theactive enzymes and growth promoting factors to generate mutual synergism and comprehensive balance to animal organisms, thus ensuring that nutrient substances can be rapidly degraded in animal intestinal canals, promoting animal to absorb and transform nutritious ingredients in the feed, and achieving the purposes of promoting animal growth and saving feed. The technology is mainly applied in animal feed promoter, such as pigs, chickens, cows and sheep and the like and biological high protein feed. The invention has easily mastered production method and process, less investment, low cost and is suitable for investing and building factories for individuals and medium-sized and small enterprises.

The present invention discloses a high density culture method and applications of probiotics. According to the present invention, a porous material is added to a culture medium containing a growth promoting factor for promoting the growth and the breeding of probiotics, a volume ratio of the culture medium to the porous material is 1:0.001-1:1000, and probiotics are inoculated in the culture medium and culturing is performed so as to achieve the high density culture of the probiotics; the obtained probiotics can be used for preparing food, food additives, medicines, feeds or environmental protection treatment, and other products; and the obtained high-density probiotics have advantages of good heat resistance, good acid resistance , good drug resistance and other performances, and have wide application prospects in food, medicines, feeds, environmental protection, and other fields.

The invention discloses compound biological protein feed and a preparation method thereof. The protein feed is made by mixing and stirring the following materials with microbial strains, fermenting, baking and crushing. The feed comprises the following materials by the weight percentages as follows: chaff 30%-55%, rice pericarp 3%-15%, and edible mushroom leftover germ bran 40%-60%. The weight ratio of the materials to the microbial strains is (90-95):(5-15). In the invention, the chaff, the edible mushroom leftover germ bran and the rice pericarp are complexly fermented to complement each other's advantages, xylogen and cellulose are degraded by 70% or so, mycoprotein is increased by 25-28%, thereby meeting the quality requirement for grain feed, and meanwhile increasing diseases suppressing and growth promoting factors, such as various vitamins, minerals, and a plurality of organized enzymes. Therefore, the protein feed is safe and environment-friendly, and can meet the growth and development requirements of livestock and poultry without addition of grain for energy.

The invention relates to the technical field of biological pharmacy, in particular to an anti-bacterial peptide, an anti-bacterial peptide hydrogel and a preparation method of the anti-bacterial peptide hydrogel. The amino acid sequence of the anti-bacterial peptide is shown as SEQ ID NO:1. Experiments show that the polypeptide of the anti-bacterial peptide has high antibacterial activity and excellent biocompatibility. The anti-bacterial peptide hydrogel is made of the anti-bacterial peptide, a photo-thermal reagent and a wound growth promoting factor. Tests show that the anti-bacterial peptide hydrogel has a good killing effect on bacteria, especially drug-resistant bacteria, on the surface of a biological film, a bacterial biofilm can be effectively spitted and removed, growth and proliferation of wound epidermal cells, collagen formation and angiogenesis are facilitated, and rapid healing of wounds infected by the bacterial biofilm is achieved.

The invention provides a method using composite bait of microalgae and other microorganisms to culture artemia. The method comprises the following steps of (1) preparing composite bait of microalgae and other microorganisms; (2) hatching nauplius of the artemia, and separating to obtain the primarily hatched nauplius of the artemia after hatching for 24 hours; (3) inoculating and culturing the primarily hatched nauplius of the artemia, wherein an artemia culturing solution is inoculated with the microalgae; (4) culturing the artemia, feeding other microorganisms for the first time after the primarily hatched nauplius of the artemia is inoculated for 24 hours, and maintaining the concentration of the microalgae all the time. The method has the advantages that the culturing is realized by a one-step food chain type, rich nutrients are provided, the culturing is convenient, the poison and harmfulness are avoided, and the cost is very low; the composite bait of microalgae and other microorganisms contains various types of growth promoting factors, so the resistant capability of the artemia is improved, the growth is quick, and the culturing density reaches 1000-10,000ind / L.

The invention provides a neural restoration sleeve and a preparing method and application thereof. The neural restoration sleeve is a double-layer fiber sleeve, the inner layer of the sleeve is an orientation polymer fiber layer, the outer layer of the sleeve is a non-orientation polymer fiber layer, bioresorbable polymers are adopted, and the nerve growth promoting factors are embedded in the inner layer. The neural restoration sleeve prepared through the preparing method can induce nerve cells to grow in the fiber orientation, meanwhile, the nerve growth promoting factors are embedded in the inner layer to promote growth of nerve axon and accelerate nerve tissue restoration, the non-woven non-orientation polymer fiber layer on the outer layer plays a role in supporting and endows the neural restoration sleeve with high strength and stability, and the polymers on the inner layer and the outer layer are the bioresorbable polymers, have high biocompatibility, will not cause chronic inflammation reaction and are high in safety; the preparing method is simple, and industrial production can be realized.

The invention discloses a novel anti-virus microorganism revivifier capable of resisting continuous cropping. The technical proportioning scheme is as follows: the revivifier comprises the following components: 30% of trichoderma, 30% of Streptomyces Microflavus, 15% of DA-6, 15% of compound sodium nitrophenolate, 5% of bacillus subtilis and 5% of Bacillus cereus. In the invention, multiple beneficial microorganisms are used for degerming and supplying trace elements taken away by crops due to continuous cropping, so that the disease caused by continuous cropping can be prevented well; and the colonization ability of the revivifier is strong, the microorganisms can be mutually promoted and secrete multiple growth-promoting factors of plants, thus, the revivifer has remarkable significance to the health growth and good harvest of the plants while overcoming the continuous cropping of the crop.

The invention relates to a method for culturing an agaricus bisporus strain by using agaricus bisporus stockpile water extract liquid. The method is characterized in that a solid culture medium for preparing a flat strain and a liquid culture medium for preparing a shake flask strain and a liquid strain both contain agaricus bisporus stockpile water extract liquid. The stockpile material of agaricus bisporus cultivation material is easily available and contains abundant nutrition as well as lots of unknown growth promoting factors; and by adding the agaricus bisporus stockpile water extract liquid into the culture medium, the growth and gene expression of agaricus bisporus hyphae can be promoted, the growth speed and the growth amount of hyphae are remarkably increased, the production cycle of solid strain and liquid strain is shortened, and the economic and social benefits of agaricus bisporus production are increased.

The invention discloses a culture method of probiotics. The culture method comprises the following steps: adding a porous material into a culture medium containing a growth-promoting factor capable ofpromoting the growth and proliferation of the probiotics, wherein the volume ratio of the culture medium to the porous material ranges from (1 to 0.001) to (1 to 1000); then inoculating the probiotics into the culture medium and culturing, so as to realize high-density culture of the probiotics. The obtained probiotics can be used for preparing products of medicines, feed or environment-protection treatment and the like. The probiotics obtained by the culture method have good performance including heat resistance, acid resistance, drug resistance and the like and have a wide application prospect in the fields of medicines, feed, environment protection and the like.

The invention relates to the technical field of poultry forages, and especially relates to a laying hen forage additive. The forage additive comprises the following compositions in per 1 Kg of a basic forage: 200-360 g of beneficial bacteria, 100-200 g of organic selenium, 20-30 g of cellulose, 10-30 g of phytase, 20-40 g of amylase, 20-40 g of oligosaccharide, 10-20 g of growth-promoting factors, 10-30 g of lactic acid, 10-20 g of malic acid, 6-10 g of sorbic acid and 10-20 g of radix astragali extract product. By employing the technical scheme, the beneficial effects comprise that the laying hen forage additive is capable of improving egg laying capability of laying hens, improving immunity of laying hens, enhancing stability of laying hens, reducing fat content in eggs, prolonging the storage time of eggs, avoiding usage of antibiotic and other harmful chemical substances and avoiding residual of harmful substances in eggs, and body immunity is enhanced after a person eats the eggs for a long time.

The invention discloses a preparing method of hepatocyte-accelerating bios liquid drugs injection agent with high stability, which comprises the following steps: adding nutria chloridum; injecting water to dilute with 9-11.5g peptide and 5-20g nutria chloridum stabilizer in each 1000ml diluted liquid; filtering; packing.

The invention provides a biomimetic artificial bone and a synthesis method. The biomimetic artificial bone consists of 20-80% of a high polymer material by mass, 5-60% of a non-degradable or partially degradable inorganic matter by mass and 5-40% of a degradable organic matter. The method comprises such steps as implementing shaping, implementing sintering and the like. The biomimetic artificial bone provided by the invention is excellent in biocompatibility. The biomimetic artificial bone, which is consistent with or similar to a part where the biomimetic artificial bone is implanted in elasticity modulus, can avoid stress shielding when implanted for a long time. The biomimetic artificial bone is sufficient in rigidity. Inner and outer layers of the biomimetic artificial bone can be honeycombed tissue structures which are provided with enough gaps, so that growth-in of related tissue cells, blood vessels, lymphatic ducts and nerves is promoted and reconstruction of blood circulation is achieved. The biomimetic artificial bone, which contains a bone growth promoting factor, is conducive to rapid healing of an implant and primitive bone.

The invention discloses an activity check method of hepatocyte growth-promoting factor, which uses RPMI-1640 culture solution. The inventive method comprises agent preparation step, sample solution preparation, sample group 3-pore average absorbance test, control group 3-pore average absorbance test and blank control group 3-pore average absorbance test and excite index calculation. The invention is characterized in that the RPMI-1640 culture solution is the one without glutamine. The invention overcomes the defects of prior art as instable test and low repeated measurement consistency, with stable test method, reliable test result, high test data repetition rate and better consistency, while the test result can correctly present sample activity.

The invention discloses a liquid eyeliner with an effect of promoting eyelash growth, comprising the following components: (1) cell growth factor components such as an acid or basic fibroblast growth factor, a dual-directional regulation growth promoting factor and an insulin-like growth factor; (2) a component for dilating the vessel such as Codariocalyx motorius saponin extract; (3) components for promoting the drug absorption such as propylene glycol, azone and menthol; (4) a component for regulating the acid-base balance; (5) bacterial growth inhibitor; and (6) the balance of deionized water. The liquid eyeliner is obtained through dissolving or mixing the above components in the deionized water and uniformly stirring. The liquid eyeliner disclosed by the invention has the advantages that the effect of promoting the eyelash growth is very obvious; the effect is very satisfying through wide trial because the eyelash growth can be seen in two weeks and the length, the thickness and the density can grow by about 1 time in four months; the growth effect is more satisfying to patients who have sparse eyelashes or lose eyelashes because of eyelash grafting; and certain effect on promoting the eyebrow growth is further provided.

The invention discloses a biological feed mainly composed of sugarcane tail leaf and a preparation method thereof. The sugarcane tail leaf is taken as the main raw material, bran, soybean meal, and fish meal are taken as the auxiliary materials, and then the materials are fermented by active 99 biological bacteria so as to produce the biological feed. The sources of raw materials are wide, the prices of raw materials are low, the manufacturing technology is simple, and the biological fertilizer is rich in various beneficial microbes, which can form strong dominant populations with the beneficial bacteria in the gastrointestinal tracts after the feed is eaten by animals, are capable of promoting protein synthesis in organisms, and can secrete and synthesize a great amount of amino acid, protein, vitamin, various digestion enzymes, micro growth promoting factors, and the like. At the same time, the active substances secreted by the beneficial microbes can prominently strengthen the immunity of animals, the using frequency of veterinary drug is greatly reduced, or the veterinary drug is not needed, and the quality of animals is greatly improved. The provided biological feed has the advantages of low cost, comprehensive nutrients, suitability for various animals, and good feeding effect.

The invention discloses a culture method of probiotics capable of improving intestinal flora of pregnant and lying-in women and application. A porous material is added into a culture medium containinga growth-promoting factor capable of promoting the growth and proliferation of the probiotics; the volume ratio of the culture medium to the porous material ranges from (1 to 0.001) to (1 to 1000); then the probiotics are inoculated in the culture medium and are cultured, so that high-density culture of the probiotics is realized; the obtained probiotics can be used for preparing products including foods, food additives, medicines and feed or can be used for carrying out environment-protection treatment and the like. The high-density probiotics prepared by the culture method have good performance including heat resistance, acid resistance, drug resistance and the like and have a wide application prospect in the field of foods, medicines, feed, environment protection and the like.

The invention provides a technology for preparing capsule of liver cell growth promoting factor which comprises the steps of, extracting polypeptide dried powder from hepatocyte growth-promoting factors solution, charing diluent, mixing and palletizing, and loading into capsules, wherein the polypeptide dried powder extracting procedure comprises employing alcohol depositing method, charging ethanol into the solution of hepatocyte growth-promoting factors, refrigerating, separating, and collecting precipitation.

The invention provides a bone-marrow mesenchymal stem cell culture medium and application thereof, and belongs to the technical field of stem cells as well as cell culture and amplification. The bone-marrow mesenchymal stem cell culture medium consists of the following ingredients at the following concentrations: 52-53% by volume of Dulbecco modified MEM medium, 38-42% by volume of MCDB medium, 1XITS cell culture additives, 8-12 microgram / milliliter of linoleic acid, 40-60 microgram / milliliter of human serum albumin, 1X penicillin and streptomycin, 75-125 micromoles of L-ascorbic acid, 1-3% by volume of fetal bovine serum, 10-15 micromoles of dexamethasone, 12-18 ng / mL of platelet-derived growth factors, and 8-12 ng / mL of growth promoting factors. Being adopted, the bone-marrow mesenchymal stem cell culture medium provided by the invention is capable of increasing success rate of clonal culture of bone-marrow mesenchymal stem cells (bMSC); and moreover, the bone-marrow mesenchymal stem cell culture medium is capable of increasing passage times, ensuring relatively high cell purity and improving cell primitivity.

The invention discloses a processing method of cassava residues added in sheep feed. According to the processing method of the cassava residues added in the sheep feed, double-enzyme hydrolysis is performed on the cassava residues so that synergistic effects of two enzymes are utilized; moreover, simultaneous addition of the two enzymes is beneficial for transformation of starch to the maximum extent. Coarse fiber is degraded into small-molecular substances which are easy for the animals to digest and absorb, such as monosaccharides, disaccharides, amino acids and so on, by using cellulase. And then, the cassava residues are fermented by using mixed strains of yeasts and mildews so as to produce a large amount of microorganism proteins and other useful metabolites which are rich in nutrition during the biological treatment processes; and thus, the processed cassava residues contain a variety of digestive enzymes and multiple growth promoting factors so that the cassava residues are capable of enhancing disease resistance of the animals, as well as promoting growth and development of the animals. According to the processing method, the cassava residues are utilized as a raw material; and fermentation using mixed bacteria with addition of urea after a series of pretreatments, including enzymatic hydrolysis and the like, is carried out so as to obtain a bacterial protein feed with relatively high bacterial protein content.

The invention relates to a hepatocyte growth-promoting factor enteric-coated capsule which is prepared from hepatocyte growth-promoting factor and a pharmaceutically acceptable enteric-coated capsule as the auxiliary material. The invention also discloses a preparation process and application of the enteric-coated capsule. The product, which is processed by gel chromatography, has the advantages of less impurities and higher product purity. Compared with the hepatocyte growth-promoting factor in the market, the invention enhances the activity by about 70%.

The invention provides a rabeprazole sodium combined medicament and a preparation process thereof. The rabeprazole sodium combined medicament is characterized by comprising the following raw materials as components in proportion by weight: 5-10 of ilaprazole sodium, 20-30 of composition of reduced glutathione and hepatocyte growth-promoting factors in proportion by weight of 1:10, and 50-60 of diammonium glycyrrhizinate. According to a pharmaceutically allowable dose of rabeprazole sodium, pharmaceutical preparations in dosage forms of injection, lyophilized powder injection, enteric coated tablets, enteric coated capsules, spray and the like of the rabeprazole sodium combined medicament are respectively prepared for treating gastric ulcer.

A fracture structure is grown from a plurality of starting points. A fractal structure, grown from respective starting points and interconnected by interactive growths, forms a neural network. A growth speed originated at a specific starting point is determined by the probability of a material reaching a grown portion from a remote location by means of a diffusion process and the probability of a growth promoting factor reaching a grown portion by means of a diffusion process from a portion grown from a starting point other than the specific one. Anisotropy is introduced into a space in which a fractal structure is to be grown, as required.

The invention relates to biological feed, belongs to the production field of fertilizer, and particularly relates to a method for making magnetic fly maggot feed. The method is characterized in that fly maggot which is habituated and cultured in magnetic field, with the field strength being larger than 300 gauss, is contained in the magnetic bio fertilizer. The method has the benefits that the investment of fly maggot protein feed meal is little, the cost is low, the making period is short, the method is simple to operate, and industrial production is easy to perform; products can tolerate high-temperature pelletization, can be rapidly volatilized after entering intestinal tract and consume oxygen, and maintain the anaerobic environment of the intestinal tract; the products assist animals to digest and absorb the feed, and effectively inhibit the multiplication of various germs; and a plurality of amino acid, vitamin, trace elements. digestive ferment, organic acid and growth promoting factors are replenished, stomach function is promoted, and the exudation of endogenous enzyme is activated.

The invention discloses a water quality improving microbial agent capable of achieving biological modification and alga culture. The water quality improving microbial agent includes the following rawmaterials: in parts by weight, 10-15 parts of seaweed extract, 20-30 parts of water-soluble organic matter, 30-45 parts of fermented organic matter, 25-30 parts of a microporous protective agent, 3-5parts of alga growth promoting factors and 3-5 parts of a compound bacterial agent. The invention also discloses a preparation method of the water quality improving microbial agent capable of achieving biological modification and alga culture. The water quality improving microbial agent is capable of activating the activity of microorganisms in the water body and improving nutrient circulation, and has the dual effects of promotion of the biological improvement and water quality through combination of two forms of carbon sources, the seaweed extract is contained, and the water quality improving microbial agent has the effect of promotion of the growth of microalgae and water plants at low temperatures, so that excellent water color is formed quickly.

The invention discloses kelp residue seed coating pesticide fertilizer as well as a preparation method and a coating method thereof. The kelp residue seed coating pesticide fertilizer is prepared fromthe following raw materials in parts by weight: 65 to 70 parts of kelp residue, 20 to 25 parts of modified attapulgite, 0.5 to 0.8 part of compound fertilizer powder, 3 to 5 parts of a compound antibiological inoculant, 0.13 to 0.29 part of pesticide, 3 to 8 parts of agricultural neutral sodium polyacrylate, 0.7 to 0.9 part of ammonium molybdate and 0.3 to 0.6 part of ferrous sulfate. According to the kelp residue seed coating pesticide fertilizer disclosed by the invention, through improvement and reasonable proportioning on production raw materials, components of antibacterial and insect-proof organic and inorganic nutrients, growth promoting factors and the like can be generated after the kelp residue seed coating pesticide fertilizer is applied to seed coating of corn, rice or peanuts; meanwhile, the kelp residue seed coating pesticide fertilizer has the advantages that the cost is low, the validity period of nutrients is long, the alkalinity of alkaline soil can be regulated andthe like, and the germination rate and the disease resistance of seeds can be increased.