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High density culture method and applications of probiotics

A technology of high-density culture and probiotics, which is applied in the direction of bacteria, fungi, and the use of added compounds to stimulate growth, etc., can solve the problems of difficult expansion of production and application scale, consumption of a large amount of medium, etc., and achieve growth and reproduction rate. Production cost and the effect of improving production efficiency

Inactive Publication Date: 2016-12-21
杭州益儒信息科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hayakawa K et al. (J Ferment Bioeng, 1990, 70, 404) used membrane technology coupled with a fermenter to continuously filter out stress components such as lactic acid to achieve a maximum cell density of 10 11 CFU / mL, this method consumes a large amount of culture medium, and is limited to the laboratory level, it is difficult to expand to the scale of production application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Cellulose / chitosan gel is added to the culture medium, the gel porosity is 90%, and the average pore size is 500 nm. The weight percentage of each component in the medium is 75% of MRS, 1% of L-cysteine ​​hydrochloride, 1% of vitamin C, 5% of carrot juice, 10% of mushroom extract, and 8% of soy milk. The volume ratio of culture medium to porous material is 1:0.2.

[0044] Bifidobacterium was inoculated into the culture medium. Bifidobacterium was purchased from China Industrial Microbial Culture Collection and Management Center. The concentration of Bifidobacterium was 10 9 CFU / mL bacterial solution, the inoculum amount is 20%. Anaerobic culture, culture at 37°C for 16 hours, the total number of viable bifidobacteria in the product after drying can reach 8×10 11 -3×10 13 CFU / g, the number of viable cells is affected by the drying method and other factors, and the number of viable cells is higher in freeze-dried products.

Embodiment 2

[0046] The collagen / trehalose porous membrane is added to the culture medium, the porosity of the porous membrane is 80%, and the average pore size is 200 nm. The weight percentage of each component in the medium is 80% MRS, 4% sucrose, 1% magnesium salt, 5% corn juice, 5% mushroom extract, and 5% whey fermentation broth. The volume ratio of culture medium to porous material is 1:1.

[0047] Lactobacillus rhamnosus was inoculated into the culture medium. Lactobacillus rhamnosus was purchased from the public channels of the China Industrial Microbial Culture Collection and Management Center. The concentration of Lactobacillus rhamnosus was 10 9 CFU / mL bacterial solution, the inoculum amount is 30%. Incubate at 37℃ for 24h, the total number of viable bacteria in the product after drying can reach 2×10 12 -3×10 14 CFU / g, the number of viable cells is affected by the drying method and other factors, and the number of viable cells is higher in freeze-dried products.

Embodiment 3

[0049] Polylactic acid foam is added to the culture medium, the porosity of the foam is 92%, and the average size is 400 μm. The weight percentage of each component in the medium is 85% of YPD, 4% of malt extract, 1% of vitamins, 5% of oyster mushroom extract, 2% of ganoderma extract, and 3% of collagen fermentation broth. The volume ratio of culture medium to porous material is 1:500.

[0050] Yeast was inoculated into the culture medium. Yeast was purchased from the China Industrial Microbial Culture Collection and Management Center. The concentration of yeast used was 10 8 CFU / mL bacterial solution, the inoculum amount is 50%. Cultivate at 35℃ for 24h, the total number of viable bacteria in the product after drying can reach 3×10 12 -2×10 14 CFU / g, the number of viable cells is affected by the drying method and other factors, and the number of viable cells is higher in freeze-dried products.

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PUM

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Abstract

The present invention discloses a high density culture method and applications of probiotics. According to the present invention, a porous material is added to a culture medium containing a growth promoting factor for promoting the growth and the breeding of probiotics, a volume ratio of the culture medium to the porous material is 1:0.001-1:1000, and probiotics are inoculated in the culture medium and culturing is performed so as to achieve the high density culture of the probiotics; the obtained probiotics can be used for preparing food, food additives, medicines, feeds or environmental protection treatment, and other products; and the obtained high-density probiotics have advantages of good heat resistance, good acid resistance , good drug resistance and other performances, and have wide application prospects in food, medicines, feeds, environmental protection, and other fields.

Description

Technical field [0001] The invention relates to a method for culturing bacteria and its application, in particular to a method for high-density culturing of probiotic bacteria and its application. Background technique [0002] Probiotics, the English word probiotics is derived from the Greek "for live" and translated as "for life". It is a live bacteria that contains physiological activity. When taken by the body through oral or other methods of administration, the appropriate amount It can colonize the host and improve the host microecological balance, thus playing a beneficial role. Research in recent years has shown that there are more than 1,000 kinds of bacteria in the human body, especially the most abundant bacteria in the gastrointestinal tract, accounting for about 80% of the total microecological bacteria in the body, which is 10 times that of human cells. Two genomes". These bacteria help the human body to digest food, produce vitamins to prevent diseases caused by b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C12N1/16C12N1/14
CPCC12N1/38C12N1/14C12N1/16C12N1/20
Inventor 胡梦欣王晓剑
Owner 杭州益儒信息科技有限公司
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