Pipeline type photobioreactor for culturing microalgae in multi-nutrition mode and using method of pipeline type photobioreactor

Abstract

The invention discloses a pipeline type photobioreactor for culturing microalgae in multiple nutrition modes. The pipeline type photobioreactor comprises a plurality of groups of reaction units and acirculating unit, wherein each reaction unit comprises front and rear rows of transparent pipelines; the circulating unit comprises a buffer tank and a circulating pump, wherein the buffer tank comprises a tank body, a cover body and a heating jacket, a heating device is also arranged in the heating jacket, and a pH sensor and a temperature sensor are arranged in the tank body; a discharge headerpipe is arranged at the bottom of the tank body, the discharge header pipe communicates with an inlet of the circulating pump, an outlet pipeline of the circulating pump is divided into feeding branches, and the feeding branches communicate with feeding ports of the reaction units; a material collecting pipeline is arranged on each feeding branch; a discharge hole of each reaction unit communicates with the circulating main pipe through a discharge pipeline; the circulating header pipe communicates with the buffer tank; a discharge branch pipe is also arranged on the discharge header pipe; a breather valve, an inoculation port, a carbon dioxide pipeline, a nutritive salt supplementing pipeline and a turbidity testing pipeline are arranged on the cover body; and the turbidity testing pipeline communicates with a turbidity detection device.

Description

technical field

[0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a pipeline-type photobioreactor for cultivating microalgae in a multi-nutrient mode and a use method thereof. Background technique

[0002] Microalgae are a class of photoautotrophic microorganisms, and a few species of algae are also capable of heterotrophic metabolism. Algae cells are rich in nutrients and have been widely developed as health food, medicine, beauty products, etc. For example, algae oil DHA is forcibly added to infant milk powder as a dietary supplement. However, the current production capacity of microalgae is limited, which limits its wider application. Among them, the low density of microalgae culture and the low content of active substances are the main reasons for the insufficient production capacity. In the traditional microalgae culture process, due to the influence of factors such as temperature, light, and nutrient salts, th...

Examples

Embodiment 1

[0036] A pipeline type photobioreactor for cultivating microalgae in a multi-nutrient mode of the present invention includes 2 groups of reaction units and circulation units arranged at different heights of the pipe frame 35; each group of reaction units includes The number of the five transparent pipes 36 in the front row and the transparent pipes 36 in the five rear rows arranged on the pipe frame are the same and the two are arranged in a staggered manner. The transparent pipes 36 in the front row and the transparent pipes 36 in the rear row pass through Elbows are connected successively to form spiral pipes; on the pipe frame 35, an external LED light source is arranged between the transparent pipes 36 in the front row and the transparent pipes 36 in the back row or on the rear side of the transparent pipes 36. The LED light source includes several groups of LED lamp groups arranged at different heights, and the LED lamp groups include the second LED lamp 37, the third LED ...

Embodiment 2

[0050] The difference between this embodiment and Example 1 is that the method of use is the method of use when Haematococcus pluvialis is cultured concurrently;

[0051] Include the following specific steps:

[0052] 1. The reactor is sterilized for the first time including the sterilization of the buffer tank 34 and the sterilization of the reaction unit; the specific steps are to pass the steam from the circulation main pipe 28 into the buffer tank 34 successively through the steam pipeline 29, and from the discharge pipelines of each reaction unit 27 into the transparent pipe 36 of the reaction unit, steam and condensed liquid are discharged from the buffer tank 34 and the reaction unit from the discharge branch pipe 20 and the sampling pipeline 26 respectively, and the reactor sterilization operation is completed;

[0053] 2. Carry out the configuration of the concurrent culture medium, comprising the following steps: add 200L of tap water in the buffer tank 34 through th...

Embodiment 3

[0062] The difference between this embodiment and Example 1 is that the method of use is the method of use when Haematococcus pluvialis is cultured heterotrophically;

[0063] Include the following specific steps:

[0064] 1. The reactor is sterilized for the first time including the sterilization of the buffer tank 34 and the sterilization of the reaction unit; the specific steps are to pass the steam from the circulation main pipe 28 into the buffer tank 34 successively through the steam pipeline 29, and from the discharge pipelines of each reaction unit 27 into the transparent pipe 36 of the reaction unit, steam and condensed liquid are discharged from the buffer tank 34 and the reaction unit from the discharge branch pipe 20 and the sampling pipeline 26 respectively, and the reactor sterilization operation is completed;

[0065] 2. Carry out the configuration of the concurrent culture medium, comprising the following steps: add 200L of tap water in the buffer tank 34 throu...

technical field

[0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a pipeline-type photobioreactor for cultivating microalgae in a multi-nutrient mode and a use method thereof. Background technique

[0002] Microalgae are a class of photoautotrophic microorganisms, and a few species of algae are also capable of heterotrophic metabolism. Algae cells are rich in nutrients and have been widely developed as health food, medicine, beauty products, etc. For example, algae oil DHA is forcibly added to infant milk powder as a dietary supplement. However, the current production capacity of microalgae is limited, which limits its wider application. Among them, the low density of microalgae culture and the low content of active substances are the main reasons for the insufficient production capacity. In the traditional microalgae culture process, due to the influence of factors such as temperature, light, and nutrient salts, th...

Examples

Embodiment 1

[0036] A pipeline type photobioreactor for cultivating microalgae in a multi-nutrient mode of the present invention includes 2 groups of reaction units and circulation units arranged at different heights of the pipe frame 35; each group of reaction units includes The number of the five transparent pipes 36 in the front row and the transparent pipes 36 in the five rear rows arranged on the pipe frame are the same and the two are arranged in a staggered manner. The transparent pipes 36 in the front row and the transparent pipes 36 in the rear row pass through Elbows are connected successively to form spiral pipes; on the pipe frame 35, an external LED light source is arranged between the transparent pipes 36 in the front row and the transparent pipes 36 in the back row or on the rear side of the transparent pipes 36. The LED light source includes several groups of LED lamp groups arranged at different heights, and the LED lamp groups include the second LED lamp 37, the third LED ...

Embodiment 2

[0050] The difference between this embodiment and Example 1 is that the method of use is the method of use when Haematococcus pluvialis is cultured concurrently;

[0051] Include the following specific steps:

[0052] 1. The reactor is sterilized for the first time including the sterilization of the buffer tank 34 and the sterilization of the reaction unit; the specific steps are to pass the steam from the circulation main pipe 28 into the buffer tank 34 successively through the steam pipeline 29, and from the discharge pipelines of each reaction unit 27 into the transparent pipe 36 of the reaction unit, steam and condensed liquid are discharged from the buffer tank 34 and the reaction unit from the discharge branch pipe 20 and the sampling pipeline 26 respectively, and the reactor sterilization operation is completed;

[0053] 2. Carry out the configuration of the concurrent culture medium, comprising the following steps: add 200L of tap water in the buffer tank 34 through th...

Embodiment 3

[0062] The difference between this embodiment and Example 1 is that the method of use is the method of use when Haematococcus pluvialis is cultured heterotrophically;

[0063] Include the following specific steps:

[0064] 1. The reactor is sterilized for the first time including the sterilization of the buffer tank 34 and the sterilization of the reaction unit; the specific steps are to pass the steam from the circulation main pipe 28 into the buffer tank 34 successively through the steam pipeline 29, and from the discharge pipelines of each reaction unit 27 into the transparent pipe 36 of the reaction unit, steam and condensed liquid are discharged from the buffer tank 34 and the reaction unit from the discharge branch pipe 20 and the sampling pipeline 26 respectively, and the reactor sterilization operation is completed;

[0065] 2. Carry out the configuration of the concurrent culture medium, comprising the following steps: add 200L of tap water in the buffer tank 34 throu...

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