Method for culturing agaricus bisporus strain by using agaricus bisporus stockpile water extract liquid

A technology of Agaricus bisporus, water extraction, applied in mushroom cultivation, botanical equipment and methods, applications, etc., to achieve the effects of increasing mycelial growth speed and growth, promoting growth and gene expression, and enriching nutrients

Inactive Publication Date: 2016-11-23
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the medium for preparing Agaricus bisporus strains,

Method used

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  • Method for culturing agaricus bisporus strain by using agaricus bisporus stockpile water extract liquid
  • Method for culturing agaricus bisporus strain by using agaricus bisporus stockpile water extract liquid

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Embodiment Construction

[0017] The technical solutions of the present invention will be further described in detail below with reference to the embodiments, but the protection scope of the present invention is not limited thereto.

[0018] In the following examples, the used secondary fermentation of Agaricus bisporus cultivation material stack can be obtained through the following steps:

[0019] 1) Build heap

[0020] The formula of the cultivation material is: the mass ratio of wheat straw and dry chicken manure is 5:1. In order to ensure sufficient nutrients in the cultivation material, superphosphate, cake fertilizer, calcium carbonate, urea and compound fertilizer (with 2% of the mass of the cultivation material) are added. In terms of mass ratio, superphosphate: cake fertilizer: calcium carbonate: urea: compound fertilizer = 1:2:1:1:1) as an auxiliary material to increase nutrition, and at the same time add an appropriate amount of lime to adjust the pH value of the cultivation material to 7.5...

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Abstract

The invention relates to a method for culturing an agaricus bisporus strain by using agaricus bisporus stockpile water extract liquid. The method is characterized in that a solid culture medium for preparing a flat strain and a liquid culture medium for preparing a shake flask strain and a liquid strain both contain agaricus bisporus stockpile water extract liquid. The stockpile material of agaricus bisporus cultivation material is easily available and contains abundant nutrition as well as lots of unknown growth promoting factors; and by adding the agaricus bisporus stockpile water extract liquid into the culture medium, the growth and gene expression of agaricus bisporus hyphae can be promoted, the growth speed and the growth amount of hyphae are remarkably increased, the production cycle of solid strain and liquid strain is shortened, and the economic and social benefits of agaricus bisporus production are increased.

Description

technical field [0001] The invention belongs to the technical field of Agaricus bisporus cultivation, and in particular relates to a method for culturing Agaricus bisporus strains by utilizing the water extract of Agaricus bisporus piles. Background technique [0002] Soil is the base camp of microorganisms. Soil contains specific factors that are lacking in the growth and metabolism of microorganisms under laboratory conditions. Therefore, through the method of in situ culture, "unculturable microorganisms" can also grow under laboratory conditions. Adding soil water-soluble extract to the medium can increase the expression of known genes of bacteria and actinomycetes, and activate the silenced genes. However, the effects of soil extracts on fungi have not been reported. [0003] Agaricus bisporus ( Agaricus bisporus ) is the most widely cultivated edible fungus in the world with the largest production and consumption, accounting for about 3 / 4 of the world's total edible ...

Claims

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Application Information

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IPC IPC(8): C05G3/00A01G1/04
CPCA01G18/00C05B7/00C05G3/00C05D5/00C05F11/00C05F11/10
Inventor 邱立友杨潜龙高玉千黄涛田芳张朝辉
Owner HENAN AGRICULTURAL UNIVERSITY
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