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A method for cultivating Agaricus bisporus strains by utilizing the liquid extract of Agaricus bisporus stockpile

A technology of bisporus bisporus and water extraction, which is applied in the directions of mushroom cultivation, botanical equipment and methods, and application, etc., to achieve the effects of improving the growth rate and amount of mycelium, promoting growth and gene expression, and enriching nutrients.

Inactive Publication Date: 2019-11-15
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the medium for preparing Agaricus bisporus strains, adding stockpile water extract has not been reported yet

Method used

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  • A method for cultivating Agaricus bisporus strains by utilizing the liquid extract of Agaricus bisporus stockpile
  • A method for cultivating Agaricus bisporus strains by utilizing the liquid extract of Agaricus bisporus stockpile

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Embodiment Construction

[0017] The technical solutions of the present invention will be further described in detail below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.

[0018] In the following examples, the Agaricus bisporus cultivation material stack after the secondary fermentation used can be obtained through the following steps:

[0019] 1) Build a heap

[0020] The formula of the cultivation material is: wheat straw and dry chicken manure in a mass ratio of 5:1. In order to ensure sufficient nutrients in the cultivation material, calcium superphosphate, cake fertilizer, calcium carbonate, urea, and compound fertilizer (with 2% of the mass of the cultivation material) are added at the same time In terms of mass ratio, superphosphate: cake fertilizer: calcium carbonate: urea: compound fertilizer = 1:2:1:1:1) as an auxiliary material to increase nutrition, and add an appropriate amount of lime to adjust the pH value of the cultivation m...

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Abstract

The invention relates to a method for culturing an agaricus bisporus strain by using agaricus bisporus stockpile water extract liquid. The method is characterized in that a solid culture medium for preparing a flat strain and a liquid culture medium for preparing a shake flask strain and a liquid strain both contain agaricus bisporus stockpile water extract liquid. The stockpile material of agaricus bisporus cultivation material is easily available and contains abundant nutrition as well as lots of unknown growth promoting factors; and by adding the agaricus bisporus stockpile water extract liquid into the culture medium, the growth and gene expression of agaricus bisporus hyphae can be promoted, the growth speed and the growth amount of hyphae are remarkably increased, the production cycle of solid strain and liquid strain is shortened, and the economic and social benefits of agaricus bisporus production are increased.

Description

technical field [0001] The invention belongs to the technical field of Agaricus bisporus cultivation, and in particular relates to a method for cultivating Agaricus bisporus strains by utilizing the water extraction solution of the Agaricus bisporus stockpile. Background technique [0002] Soil is the base camp of microorganisms. The soil contains specific factors that are lacking in the growth and metabolism of microorganisms under laboratory conditions. Therefore, through the method of in situ cultivation, "unculturable microorganisms" can also grow under laboratory conditions. Adding soil water-soluble extracts to the medium can increase the expression of known genes of bacteria and actinomycetes and activate silenced genes. However, the effects of soil extracts on fungi have not been reported. [0003] Agaricus bisporus ( Agaricus bisporus ) is the most widely cultivated edible fungus with the largest production and consumption in the world, accounting for about 3 / 4 of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00A01G18/20A01G18/40
CPCA01G18/00C05B7/00C05G3/00C05D5/00C05F11/00C05F11/10
Inventor 邱立友杨潜龙高玉千黄涛田芳张朝辉
Owner HENAN AGRICULTURAL UNIVERSITY
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