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Culture method of probiotics

A culture method and technology of probiotics, applied in the field of microorganisms, can solve the problems of less research on probiotic solid beverages, and achieve the effects of increased growth and reproduction rate and good heat resistance

Active Publication Date: 2018-11-16
ZHONGKE YIKANG BEIJING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Helicobacter pylori is a Gram-negative bacillus, which is the only cause of chronic active gastritis and peptic ulcer, and is closely related to gastric malignant tumors. It is listed as a class I carcinogen by the World Health Organization and the International Union Against Cancer , most of the probiotic solid beverages reported in the prior art can maintain the colony balance of the intestinal tract, but there are few studies on the probiotic solid beverages against Helicobacter pylori, so it is necessary to provide a The role of probiotics solid drink

Method used

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  • Culture method of probiotics

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0066] The preparation method of this highly active probiotic solid beverage is:

[0067] (1) Stir the formula dosage of Lactobacillus helveticus powder, Lactobacillus rhamnosus powder, Bifidobacterium animal powder, Bifidobacterium lactis powder, Lactobacillus acidophilus powder and Bifidobacterium bifidum powder After homogenization, mixture A is obtained;

[0068] (2) Mix inulin, resistant dextrin and mixture A, and mix well to obtain mixture B;

[0069] (3) Mix the obtained mixture B with the remaining raw materials in the formula, and after stirring evenly, obtain the mixture D;

[0070] (4) freeze-drying the mixture obtained in step (3) to obtain the probiotic solid beverage.

[0071] The stirring speed in step (1) is 5-15r / min, and the stirring time is 30-50min.

[0072] The stirring speed in step (2) is 20-30r / min, and the stirring time is 20-40min.

[0073] The stirring speed in step (3) is 35-45r / min, and the stirring time is 10-30min.

[0074] The freeze-drying...

experiment example 1

[0099] The high-activity probiotic solid beverages prepared in Examples 1-10 and Comparative Examples 1-7 were tested for in vitro inhibition of Helicobacter pylori, and the specific steps were as follows:

[0100] 1. Materials

[0101] The strain H.CagA(+) pylori is the international standard strain NCTC11637, which is CagA(+) and VacA(+).

[0102] 2. Method

[0103] In a sterile environment, weigh 5 g of the probiotic solid beverages of Examples 1-10 and Comparative Examples 1-7, dissolve them in 200 ml of sterile water, adjust the pH of the solution to 2.0 with hydrochloric acid, stir at 40°C for 2 hours, and filter , the filtrate was rotary evaporated to a volume of less than 50ml, and then dilute to 100ml with sterile water. Then it was diluted to 1:40. A number of circular filter paper pieces with a diameter of 6 mm were punched out with a filter paper puncher, and sterilized by high-pressure steam at 120°C. Under a sterile environment, use sterile tweezers to pick u...

experiment example 2

[0110] 1. Materials: Male mice are weighed, 10 in every group, according to the highly active probiotic solid beverage provided by the embodiment of the present invention 1-10, the mice are weighed, and the mice are weighed according to 1.67g / kg.bw respectively Practice blood gavage for 10 days, and the blank group uses sterile diluent to tank the stomach. Before administering the test substance, take 0.1 g of mouse feces, add 10-fold dilution with sterile normal saline, calculate the number of bacteria in each gram of wet stool, take the logarithm and perform statistics, after the last administration of the test substance 24 , take feces aseptically, and detect the intestinal flora, the method is the same as above, and the results are shown in Table 3:

[0111]

[0112]

[0113] As can be seen from Table 3, the highly active probiotic solid beverage provided by Examples 1-10 of the present invention, after the mice were fed, the content of Bifidobacterium and Lactobacil...

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Abstract

The invention discloses a culture method of probiotics. The culture method comprises the following steps: adding a porous material into a culture medium containing a growth-promoting factor capable ofpromoting the growth and proliferation of the probiotics, wherein the volume ratio of the culture medium to the porous material ranges from (1 to 0.001) to (1 to 1000); then inoculating the probiotics into the culture medium and culturing, so as to realize high-density culture of the probiotics. The obtained probiotics can be used for preparing products of medicines, feed or environment-protection treatment and the like. The probiotics obtained by the culture method have good performance including heat resistance, acid resistance, drug resistance and the like and have a wide application prospect in the fields of medicines, feed, environment protection and the like.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for cultivating probiotics. Background technique [0002] Probiotics, the word probiotics in English is derived from the Greek word "for live", translated as "for life". It can colonize the host and improve the micro-ecological balance of the host, thereby playing a beneficial role. Studies in recent years have shown that there are more than 1,000 kinds of bacteria in the human body, especially the bacteria in the gastrointestinal tract are the most abundant, accounting for about 80% of the total number of microecological bacteria in the whole body, which is 10 times that of human cells. Two Genomes". These bacteria help the body digest food, produce vitamins to prevent diseases caused by bacteria in food, and stimulate the immune system. With the deepening of research, it has been found that gut microbes also play a key role in many chronic disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A23L2/39A23L2/52A23L33/00
CPCA23L2/39A23L2/52A23L33/00C12N1/16C12N1/20A23V2200/00A23V2400/147A23V2400/113A23V2400/175A23V2400/51A23V2400/515A23V2250/5062A23V2200/3204A23V2200/32A23V2200/324
Inventor 张烨刘海霞耿然
Owner ZHONGKE YIKANG BEIJING BIOTECH CO LTD
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