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Sheep frozen semen dilution and process used for preparing sheep straw frozen semens

A technology for making crafts and diluents, used in applications, preservation of human or animal bodies, animal husbandry, etc.

Inactive Publication Date: 2015-06-17
XINJIANG TIANSHAN ANIMAL HUSBANDRY BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, sheep semen freezing technology has made some progress at home and abroad, but it is still in the stage of experimentation and improvement

Method used

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  • Sheep frozen semen dilution and process used for preparing sheep straw frozen semens
  • Sheep frozen semen dilution and process used for preparing sheep straw frozen semens
  • Sheep frozen semen dilution and process used for preparing sheep straw frozen semens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A sheep frozen semen dilution mainly comprises the following steps:

[0060] Step 1: Preparation of base liquid:

[0061] Tris 30.44g, citric acid 17.21g, fructose 12.65g, use double distilled water to dilute to 1L as the base solution;

[0062] Step 2: Preparation of Diluent A:

[0063] Take 800mL of the above base solution, add 200mL of egg yolk, 500,000 units of streptomycin sulfate, 1.15g of ciprofloxacin, and 1.6g of gentamycin, and mix well to prepare dilution A;

[0064] Step 3: Preparation of Diluent B:

[0065] Take 890 mL of diluent A liquid obtained in step 2, add 110 mL of glycerin, and its pH value is between 6.7 and 7.2, and obtain diluent B liquid;

[0066] The diluent A liquid and the diluent B liquid obtained in the above two and three steps are the desired dilutions of frozen semen of sheep.

Embodiment 2

[0068] A sheep frozen semen dilution mainly comprises the following steps:

[0069] Step 1: Preparation of base liquid:

[0070] Tris 26.8g, citric acid 13.8g, fructose 10g, use double distilled water to dilute to 1L as the base solution;

[0071] Step 2: Preparation of Diluent A:

[0072] Take 800mL of the above-mentioned base solution, add 200mL of egg yolk, 300,000 units of streptomycin sulfate, 1.5g of ciprofloxacin, and 2.1g of gentamicin, and mix well to prepare the dilution A;

[0073] Step 3: Preparation of Diluent B:

[0074] Take 880 mL of diluent A liquid obtained in step 2, add 120 mL of glycerin, and its pH value is between 6.7 and 7.2, and obtain diluent B liquid;

[0075] The diluent A liquid and the diluent B liquid obtained in the above two and three steps are the desired dilutions of frozen semen of sheep.

Embodiment 3

[0077] A sheep frozen semen dilution mainly comprises the following steps:

[0078] Step 1: Preparation of base liquid:

[0079] Tris 29.0g, citric acid 15.0g, fructose 13g, use double distilled water to dilute to 1L as the base solution;

[0080] Step 2: Preparation of Diluent A:

[0081] Take 820mL of the above-mentioned base solution, add 180mL of egg yolk, 500,000 units of streptomycin sulfate, 1.4g of ciprofloxacin, and 2.0g of gentamicin, and mix well to prepare the dilution A;

[0082] Step 3: Preparation of Diluent B:

[0083] Take 885 mL of diluent A liquid obtained in step 2, add 115 mL of glycerin, and its pH value is between 6.7 and 7.2, and obtain diluent B liquid;

[0084] The diluent A liquid and the diluent B liquid obtained in the above two and three steps are the desired dilutions of frozen semen of sheep.

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Abstract

The invention relates to the technical field of sheep frozen semen production, in particular to sheep frozen semen dilution and a process used for preparing sheep straw frozen semens. The sheep frozen semen dilution is prepared by the following steps: 1, preparing basic liquid; 2, preparing dilution A; 3, preparing dilution B, wherein the dilution A obtained in the step 2 and the dilution B obtained in the step 3 are to-be-obtained sheep frozen semen dilution. The sheep frozen semen dilution is used in the process for preparing the sheep straw frozen semens. The process used for preparing the sheep straw frozen semens mainly comprises the following steps: 1, diluting and balancing a sheep frozen semen fluid; 2, preparing the straw frozen semens of the sheep frozen semen fluid; 3, performing freezing and warehousing. The sheep frozen semen dilution provided by the invention greatly improves the bioactivity of sheep semens, improves production quality, and is economic and practical; the process used for preparing the sheep straw frozen semens is quick and convenient to operate, economic and practical, saves time, and facilitates improvement of the semen quality of breeding sheep and increment of annual semen volume of the breeding sheep.

Description

technical field [0001] The invention relates to the technical field of sheep frozen semen production, in particular to a sheep frozen semen diluent and a production process for sheep thin tube frozen semen. Background technique [0002] The use of semen freezing technology can greatly increase the utilization rate of breeding rams, reduce the amount of breeding rams, save costs and no longer be limited by regions and time, and accelerate the pace of breed improvement. At present, some progress has been made in sheep semen freezing technology at home and abroad, but it is still in the stage of experimentation and improvement. In recent years, with the development trend of high-efficiency and large-scale animal husbandry, the research on sheep frozen semen technology has been paid special attention. The invention focuses on optimizing the formulation of the semen freezing diluent and the freezing preservation procedure, aiming at establishing a set of simple and efficient she...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 孙红晨曹莉萍李宗方陈思宏朱兵山刘磊夏婷婷
Owner XINJIANG TIANSHAN ANIMAL HUSBANDRY BIO ENG
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