Intelligent chimeric cell culture cabin

Abstract

The invention belongs to the technical field of cell culture, and particularly relates to an intelligent chimeric cell culture cabin which is characterized by comprising an outer cabin body and a culture mechanism mounted above the outer cabin body, the fan rotates to suck air outside the device into the culture cabin through the air inlet, and in the process of blowing the air into the culture cabin, the heating pipe can heat the sucked air to increase the temperature of the air, so that the temperature of the air sucked into the device adapts to the temperature required by cell culture, and the situation that cell culture is affected by improper temperature is avoided; the sucked air is discharged out of the device through the air outlet after being circulated in the culture cabin body, so that the air in the culture cabin body can be circulated, meanwhile, waste gas generated by culturing cells in the culture cabin body is taken away, and angle openings of the air dissipation openings are staggered, so that the air discharged from the air dissipation openings can face different angles, and the air dissipation effect is improved. And the situation that the blown airflow faces the same angle and is too intense is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to an intelligent chimeric cell culture chamber. Background Art

[0002] Cell culture is a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. As the most core and basic technology in biotechnology, cell culture is widely used in research and production in biology, medicine and other fields. Through cell culture technology, a cell can be cultured in large quantities to become a simple single cell or a multi-cell with very little differentiation. Cell culture is also an important means to study processes such as cell signal transduction, metabolism, growth and proliferation.

[0003] However, the cell culture chamber of the prior art is not stable enough when the culture dish is placed during use. At the same time, the installation of the top cover of the culture dish and the culture dish is not stable enough and inconvenient to use. It does not meet some needs and needs to develop a smart chimeric cell culture chamber with new functions.

[0004] For this purpose, an intelligent chimeric cell culture chamber is needed. Summary of the invention

[0005] The intelligent chimeric cell culture chamber proposed in the present invention solves the problems in the prior art that the culture dishes are prone to shaking and are not stable when the culture dishes are placed in the cell culture chamber during use, and the top cover of the culture dish and the installation of the culture dish are not stable enough and inconvenient to use.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] An intelligent chimeric cell culture chamber comprises an outer chamber body and a culture mechanism installed above the outer chamber body, wherein the culture mechanism comprises a mounting assembly;

[0008] The installation assembly includes an air inlet, an air inlet is opened on the right rear side of the outer cabin body, a fan is installed in front of the air inlet through the outer frame body, a heating pipe is installed in front of the fan, and air dispersion outlets with different directions are opened in front of the heating pipe, a culture cabin is opened in the front of the bottom of the outer cabin body, an air outlet is opened on the left rear side of the outer cabin body, slide grooves are opened on the left and right sides of the inner wall of the culture cabin body, a slider is installed inside the slider, pulleys are installed in an array on the upper and lower sides of the slider, a support plate is fixedly installed on the side of the slider, and a baffle is fixedly installed in front of the front.

[0009] The inner array of the support plate is provided with a mounting groove, a splint is installed inside the mounting groove, a rotating shaft is installed in the middle of the splint, a spring is fixedly installed on the bottom of the splint, a culture dish is installed on the right side of the splint, a limit plate matching the size of the splint is installed on the bottom of the culture dish, a top cover is installed on the top of the culture dish, four groups of buckles are installed in the side array of the top cover, blocks are installed at positions corresponding to the buckles on the top of the culture dish, limiting buckles are installed at positions between the blocks, a mounting thread is opened at the center of the top cover, the internal mounting block is installed on the side of the mounting thread through a threaded structure, a bearing is installed inside the internal mounting block, an inner plate is installed inside the bearing, an air outlet pipe is fixedly installed on the right side of the inner plate, a one-way valve is installed inside the air outlet pipe, a liquid inlet pipe is installed at a position symmetrical to the air outlet pipe inside the inner plate, an air inlet pipe is fixedly installed behind the air outlet pipe, and a control valve is installed inside the air inlet pipe.

[0010] A pump body is fixedly installed on the top of the liquid inlet pipe, and the top of the pump body is connected to the liquid box through the liquid inlet pipe. Four groups of liquid boxes are installed in the array, and a liquid inlet is installed on the top of the liquid box. An air pump is installed on the top of the air inlet pipe, and the other side of the air pump is connected to the gas mixing chamber through the air inlet pipe. A spiral tube is fixedly installed inside the gas mixing chamber, and the right side of the gas mixing chamber is connected to the carbon dioxide gas pipe, and other gas pipes are connected to the bottom middle of the carbon dioxide gas pipe.

[0011] Preferably, the air dispersion outlets are distributed in an array in front of the heating tube, the opening directions of the air dispersion outlets are staggered, the air inlet forms a flow structure through the air dispersion outlet and the air outlet, the support plate forms a sliding structure with the slide groove through the cooperation between the slider and the pulley, and the support plate forms a detachable structure with the outer cabin body through the cooperation between the slider and the slide groove.

[0012] Preferably, the culture dish forms a detachable structure with the support plate through the cooperation between the limiting plate and the clamping plate, the clamping plate forms a rotating structure with the support plate through the rotating shaft, the rotating shaft forms a pressing structure with the support plate through the spring, and the clamping plate is a "C"-shaped structure.

[0013] Preferably, the top cover forms a snap-fit ​​structure with the culture dish through the cooperation between the snap buckle and the snap block, and the sizes of the snap buckle and the limiting buckle match each other.

[0014] Preferably, the internal mounting block forms a detachable structure with the top cover through the mounting threads, and the inner plate forms a rotating structure with the internal mounting block through the bearings.

[0015] Preferably, the culture dishes are provided in four groups, and the four groups of culture dishes are all connected to the air pump via an air inlet pipe, and a control valve is installed at one end of the air inlet pipe close to the culture dish.

[0016] Preferably, the four groups of liquid boxes are interconnected with the culture dishes through liquid inlet pipes, and the ends of the liquid inlet pipes connected to the liquid boxes are equipped with control valves. The four groups of culture dishes are connected to the pump body through liquid inlet pipes, and the ends of the liquid inlet pipes connected to the culture dishes are equipped with air inlet pipes.

[0017] Preferably, the spiral tube is fixedly installed inside the gas mixing chamber, the spiral tube has a spiral structure, the center of the spiral tube coincides with the center of the gas mixing chamber, the carbon dioxide air pipe and other gas air pipes are fixed to each other, the carbon dioxide air pipe and other gas air pipes are in communication with the spiral tube, and the liquid inlet pipe and the air inlet pipe are both hoses.

[0018] The present invention proposes an intelligent chimeric cell culture chamber. Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. During use, the fan rotates to suck the gas outside the device into the culture chamber through the air inlet. In the process of blowing into the culture chamber, the heating tube heats the inhaled air to increase the temperature of the air, so that the temperature of the air inhaled into the device adapts to the temperature required for cell culture, avoiding the influence of inappropriate temperature on cell culture, and then the inhaled air circulates inside the culture chamber and then discharges the device through the air outlet, so that the air inside the culture chamber can circulate, and at the same time, the waste gas generated by the cultured cells inside the culture chamber can be taken away, so as to maintain the air quality inside the culture chamber, and then the angles of the diffuser openings are staggered with each other, so the gas discharged from the diffuser will be directed at different angles, avoiding the airflow being blown out at the same angle, resulting in the airflow being too intense.

[0020] 2. During use, the splint will rotate through the middle shaft. The culture dish will squeeze the bottom of the splint when installed. During the squeezing process, the bottom of the splint will shrink through the spring. During the shrinking process, the top of the splint will rotate toward the side of the culture dish through the shaft. After the culture dish is placed at the bottom, the splint will clamp the top of the culture dish under the squeezing of the culture dish to prevent the culture dish from shaking inside the device and affecting its use. At the same time, the limit plate at the bottom of the culture dish will be stuck at the bottom of the splint to further improve the stability of the culture dish.

[0021] 3. During use, the internal mounting block is fixed at the center of the top cover through the mounting threads. When the internal mounting block is installed inside the mounting threads by rotation, the bearing installed inside the internal mounting block will prevent the inner plate from rotating synchronously, and prevent the pipes on the top of the inner plate from being entangled with each other, affecting the use of the device, thereby improving the overall installation flexibility and simplicity of the device.

[0022] 4. During use, the carbon dioxide required for cell culture is introduced into the gas mixing chamber through other gas pipes and the carbon dioxide pipe, and then the concentration of carbon dioxide is adjusted inside the gas mixing chamber. If the carbon dioxide concentration is too high, other gases are introduced through other gas pipes, and then mixed inside the gas mixing chamber. After mixing, the regulated gas is introduced into the culture dish through the air inlet pipe by an air pump. The air inlet pipe is connected to each culture dish through a connecting branch pipe, and a control valve is installed at the position where each branch pipe is connected to the culture dish. Therefore, it can avoid the gas inside the culture dish from flowing back into the interior of the air inlet pipe to cause gas pollution, and can also control the amount of carbon dioxide required to be introduced into different culture dishes. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the overall structure of the intelligent chimeric cell culture chamber of the present invention.

[0024] Figure 2 It is a schematic diagram of the cross-sectional structure of the outer frame of the intelligent chimeric cell culture chamber of the present invention.

[0025] Figure 3 It is a schematic diagram of the top cross-sectional structure of the outer frame of the intelligent chimeric cell culture chamber of the present invention.

[0026] Figure 4 Schematic diagram of the internal structure of the intelligent chimeric cell culture chamber of the present invention.

[0027] Figure 5 It is a schematic diagram of the cross-sectional structure of the culture chamber body of the intelligent chimeric cell culture chamber of the present invention.

[0028] Figure 6 It is a schematic diagram of the air inlet structure of the intelligent chimeric cell culture chamber of the present invention.

[0029] Figure 7 It is a schematic diagram of the internal right view structure of the intelligent chimeric cell culture chamber of the present invention.

[0030] Figure 8 It is a schematic diagram of the internal component style structure of the intelligent chimeric cell culture chamber of the present invention.

[0031] Fig. 9 It is a schematic diagram of the support plate structure of the intelligent chimeric cell culture chamber of the present invention.

[0032] Fig.10 Schematic diagram of the culture dish installation structure of the intelligent chimeric cell culture chamber of the present invention.

[0033] Fig.11 Schematic diagram of the culture dish structure of the intelligent chimeric cell culture chamber of the present invention.

[0034] Fig.12 It is a schematic diagram of the engagement structure of the culture dish and the top cover of the intelligent chimeric cell culture chamber of the present invention.

[0035] Fig.13 This is a schematic diagram of the internal installation block structure of the intelligent chimeric cell culture chamber of the present invention.

[0036] Fig.14 Schematic diagram of the gas mixing chamber structure of the intelligent chimeric cell culture chamber of the present invention.

[0037] In the figure: 1. outer cabin; 2. air inlet; 3. fan; 4. heating tube; 5. culture cabin; 6. air outlet; 7. slide groove; 8. slider; 9. pulley; 10. support plate; 11. baffle; 12. mounting groove; 13. splint; 14. rotating shaft; 15. spring; 16. culture dish; 17. limit plate; 18. top cover; 19. buckle; 20. block; 21. limit buckle; 22. mounting thread; 23. internal mounting block; 24. bearing; 25. inner plate; 26. air outlet pipe; 27. one-way valve; 28. air inlet pipe; 29. ​​control valve; 30. liquid inlet pipe; 31. pump body; 32. liquid box; 33. liquid inlet; 34. air pump; 35. gas mixing chamber; 36. spiral tube; 37. carbon dioxide air pipe; 38. other gas air pipes; 39. air outlet. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] See also Figure 1-14 , the present invention provides a technical solution: an intelligent chimeric cell culture chamber, comprising an outer chamber body 1 and a culture mechanism installed above the outer chamber body 1, the culture mechanism comprising a mounting assembly;

[0040] The installation assembly includes an air inlet 2, a fan 3, a heating tube 4, a culture chamber 5, an air outlet 6, a chute 7, a slider 8, a pulley 9, a support plate 10, a baffle 11 and an air dispersion vent 39. An air inlet 2 is provided on the right rear side of the outer chamber 1, a fan 3 is installed in front of the air inlet 2 through an outer frame, a heating tube 4 is installed in front of the fan 3, and air dispersion vents 39 with different directions are provided in front of the heating tube 4. A culture chamber 5 is provided in front of the bottom of the outer chamber 1, an air outlet 6 is provided on the left rear side of the outer chamber 1, a chute 7 is provided on the left and right sides of the inner wall of the culture chamber 5, a slider 8 is installed inside the slider 8, pulleys 9 are installed in an array on the upper and lower sides of the slider 8, a support plate 10 is fixedly installed on the side of the slider 8, and a baffle 11 is fixedly installed in front of the front.

[0041] The support plate 10 has an internal array with a mounting groove 12, a clamping plate 13 is installed inside the mounting groove 12, a rotating shaft 14 is installed in the middle of the clamping plate 13, a spring 15 is fixedly installed at the bottom of the clamping plate 13, a culture dish 16 is installed on the right side of the clamping plate 13, a limiting plate 17 that matches the size of the clamping plate 13 is installed at the bottom of the culture dish 16, a top cover 18 is installed on the top of the culture dish 16, four sets of buckles 19 are installed on the side array of the top cover 18, a clamping block 20 is installed at the position corresponding to the buckle 19 on the top of the culture dish 16, and a position between the clamping blocks 20 is installed A limiting buckle 21 is provided, and a mounting thread 22 is provided at the center of the top cover 18. An internal mounting block 23 is installed on the side of the mounting thread 22 through a threaded structure. A bearing 24 is installed inside the internal mounting block 23, and an inner plate 25 is installed inside the bearing 24. An air outlet pipe 26 is fixedly installed on the right side of the inner plate 25, and a one-way valve 27 is installed inside the air outlet pipe 26. A liquid inlet pipe 30 is installed inside the inner plate 25 at a position symmetrical to the air outlet pipe 26, and an air inlet pipe 28 is fixedly installed behind the air outlet pipe 26, and a control valve 29 is installed inside the air inlet pipe 28.

[0042] A pump body 31 is fixedly installed on the top of the liquid inlet pipe 30, and the top of the pump body 31 is connected to the liquid box 32 through the liquid inlet pipe 30. Four groups of liquid boxes 32 are installed in an array, and a liquid inlet 33 is installed on the top of the liquid box 32. An air pump 34 is installed above the air inlet pipe 28. The other side of the air pump 34 is connected to the mixing chamber 35 through the air inlet pipe 28. A spiral tube 36 is fixedly installed inside the mixing chamber 35. The right side of the mixing chamber 35 is connected to the carbon dioxide air pipe 37. Other gas air pipes 38 are connected to the bottom middle of the carbon dioxide air pipe 37.

[0043] Furthermore, the air vents 39 are arranged in an array in front of the heating tube 4, and the opening directions of the air vents 39 are staggered with each other. The air inlet 2 forms a flow structure with the air outlet 6 through the air vents 39. The support plate 10 forms a sliding structure with the slide groove 7 through the cooperation between the slider 8 and the pulley 9. The support plate 10 forms a detachable structure with the outer cabin 1 through the cooperation between the slider 8 and the slide groove 7. During use, the fan 3 rotates to suck the gas outside the device into the interior of the culture cabin 5 through the air inlet 2. In the process of blowing into the interior of the culture cabin 5, the heating tube 4 heats the inhaled air to increase the temperature of the air, so that the temperature of the air inhaled into the device adapts to the temperature required for cell culture, avoiding the influence of inappropriate temperature on cell culture, and then the inhaled air circulates inside the culture cabin 5 and is discharged from the device through the air outlet 6, so that the air inside the culture cabin 5 can be ventilated. Circulation, and at the same time, it can also take away the exhaust gas generated by the cultured cells inside the culture chamber 5, maintain the air quality inside the culture chamber 5, and then the angle openings of the air vents 39 are staggered with each other, so the gas discharged from the air vents 39 will be directed to different angles, avoiding the airflow being blown out at the same angle causing the airflow to be too intense. During use, the support plate 10 and the baffle 11 are fixed to each other, and then the support plate 10 is retracted and extended by the pulling of the baffle 11, so that the user can take out the cells cultured above the support plate 10. During the pulling process, the support plate 10 will slide inside the slide groove 7 through the side slider 8 and the pulley 9 to retract and extend. Pulleys 9 are installed in an array on the upper and lower sides of the slider 8, which can improve the stability of the support plate 10 during the pulling process and avoid the support plate 10 from tilting or shaking, which causes the cells cultured inside the culture dish 16 to be affected during the pulling process.

[0044] Furthermore, the culture dish 16 forms a detachable structure with the support plate 10 through the cooperation between the limit plate 17 and the splint 13, the splint 13 forms a rotating structure with the support plate 10 through the rotating shaft 14, and the rotating shaft 14 forms a pressing structure with the support plate 10 through the spring 15. The splint 13 is a "C"-shaped structure. During use, the splint 13 will rotate through the middle rotating shaft 14. The culture dish 16 will squeeze the bottom of the splint 13 when installing. During the squeezing process, the bottom of the splint 13 will shrink through the spring 15. During the shrinking process, the top of the splint 13 will rotate toward the side of the culture dish 16 through the rotating shaft 14. After the culture dish 16 is placed at the bottom, the splint 13 clamps the top of the culture dish 16 under the squeezing of the culture dish 16 to prevent the culture dish 16 from shaking inside the device and affecting its use. At the same time, the limit plate 17 at the bottom of the culture dish 16 will be stuck at the bottom of the splint 13, further improving the stability of the culture dish 16.

[0045] Furthermore, the top cover 18 forms a snap-fit ​​structure with the culture dish 16 through the cooperation between the buckle 19 and the block 20, and the sizes of the buckle 19 and the limiting buckle 21 are consistent with each other. During use, the top cover 18 is fixed on the top of the culture dish 16 by the snap-fitting 19 on the side. During installation, the top cover 18 is snap-fitted into the interior of the block 20 through the shape of the buckle 19, and then the limiting buckle 21 is snapped into the gap in the middle of the buckle 19, thereby fixing the buckle 19 to the culture dish 16 to prevent the top cover 18 from falling from the inside of the device and affecting the cell culture inside the culture dish 16.

[0046] Furthermore, the internal mounting block 23 forms a detachable structure with the top cover 18 through the mounting thread 22, and the inner plate 25 forms a rotating structure with the internal mounting block 23 through the bearing 24. During installation, the internal mounting block 23 is fixedly installed at the center of the top cover 18 through the mounting thread 22. When the internal mounting block 23 is installed inside the mounting thread 22 by rotation, the bearing 24 installed inside the internal mounting block 23 will prevent the inner plate 25 from rotating synchronously, and prevent the pipes on the top of the inner plate 25 from being entangled with each other, affecting the use of the device, thereby improving the overall installation flexibility and simplicity of the device.

[0047] Furthermore, four groups of culture dishes 16 are provided, and the four groups of culture dishes 16 are connected to the air pump 34 through the air inlet pipe 28. A control valve 29 is installed at one end of the air inlet pipe 28 close to the culture dish 16. During use, carbon dioxide required for cell culture is introduced into the mixing chamber 35 through other gas air pipes 38 and carbon dioxide air pipes 37, and then the concentration of carbon dioxide is adjusted in the mixing chamber 35. If the carbon dioxide concentration is too high, other gases are introduced through other gas air pipes 38, and then mixed in the mixing chamber 35. After mixing, the regulated gas is introduced into the culture dish 16 through the air inlet pipe 28 through the air pump 34. The air inlet pipe 28 is connected to each culture dish 16 through a connecting branch pipe, and a control valve 29 is installed at the position where each branch pipe is connected to the culture dish 16. Therefore, it can be avoided that the gas inside the culture dish 16 flows back into the interior of the air inlet pipe 28 to cause gas pollution, and the amount of carbon dioxide required to be introduced into different culture dishes 16 can also be controlled.

[0048] Furthermore, the spiral tube 36 is fixedly installed inside the mixing chamber 35. The spiral tube 36 has a spiral structure. The center of the spiral tube 36 and the mixing chamber 35 coincide with each other. The carbon dioxide air pipe 37 and other gas air pipes 38 are fixed to each other. The carbon dioxide air pipe 37 and other gas air pipes 38 are in communication with the spiral tube 36. The liquid inlet pipe 30 and the air inlet pipe 28 are both hoses. The gas introduced into the mixing chamber 35 from the carbon dioxide air pipe 37 will be mixed. The gas introduced into the mixing chamber 35 will hit the spiral tube 36, thereby breaking up the airflow to facilitate the mixing of different gases. At the same time, the carbon dioxide air pipe 37 and other gas air pipes 38 are interconnected with the mixing chamber 35 to facilitate the introduction of different gases into the mixing chamber 35 for mixing. The mixed gas is then introduced into different culture dishes 16 through the air pump 34.

[0049] Further, the four groups of liquid boxes 32 are connected to the culture dishes 16 through the liquid inlet pipe 30, and the ends of the liquid inlet pipe 30 connected to the liquid box 32 are installed with a control valve 29. The four groups of culture dishes 16 are connected to the pump body 31 through the liquid inlet pipe 30, and the ends of the liquid inlet pipe 30 connected to the culture dishes 16 are installed with an air inlet pipe 28. During use, four groups of culture dishes 16 are installed in an array above the support plate 10, so that multiple culture dishes 16 can be used for cell culture at the same time. During the culture process, the liquid box 32 can be placed inside The nutrients required for different cell cultures are then introduced into the culture dish 16 through the liquid inlet pipe 30 and the pump body 31 by the liquid box 32 for cell culture. At the same time, a control valve 29 is installed at the connection between the liquid inlet pipe 30 and the culture dish 16 to control the type of nutrients introduced into the liquid inlet pipe 30 by the liquid box 32. Then, a control valve 29 is also installed at the position of the liquid inlet pipe 30 close to the culture dish 16 to control the amount of nutrients introduced, so as to facilitate the control and adjustment of the culture environment inside the culture dish 16.

[0050] Working principle: First, the culture dish 16 is installed above the support plate 10, and the splint 13 is rotated through the middle shaft 14. The culture dish 16 will squeeze the bottom of the splint 13 when installed. During the squeezing process, the bottom of the splint 13 will shrink through the spring 15. During the shrinking process, the top of the splint 13 will rotate toward the side of the culture dish 16 through the shaft 14. After the culture dish 16 is placed at the bottom, the splint 13 clamps the top of the culture dish 16 under the squeezing of the culture dish 16 to prevent the culture dish 16 from shaking inside the device and affecting its use. At the same time, the limit plate 17 at the bottom of the culture dish 16 will be stuck at the bottom of the splint 13 to further improve the stability of the culture dish 16.

[0051] The top cover 18 is fixed on the top of the culture dish 16 by means of the buckle 19 on the side. During installation, the top cover 18 is buckled into the inside of the clamping block 20 by means of the shape of the buckle 19, and then the limit buckle 21 is buckled into the gap in the middle of the buckle 19, thereby fixing the buckle 19 to the culture dish 16 to prevent the top cover 18 from falling from the inside of the device and affecting the cell culture inside the culture dish 16. After the installation is completed, the internal mounting block 23 is fixedly mounted at the center of the top cover 18 by means of the mounting thread 22. When the internal mounting block 23 is mounted inside the mounting thread 22 by means of rotation, the bearing 24 installed inside the internal mounting block 23 prevents the inner plate 25 from rotating synchronously, and prevents the pipes on the top of the inner plate 25 from being entangled with each other, which affects the use of the device, thereby improving the overall installation flexibility and simplicity of the device.

[0052] Then, the air inlet pipe 28 and the liquid inlet pipe 30 are connected to the top of the inner plate 25. After the connection is completed, the carbon dioxide required for cell culture is introduced into the mixing chamber 35 through other gas pipes 38 and the carbon dioxide pipe 37, and then the concentration of carbon dioxide is adjusted in the mixing chamber 35. If the carbon dioxide concentration is too high, other gases are introduced through other gas pipes 38, and then mixed in the mixing chamber 35. The gas introduced into the mixing chamber 35 by the carbon dioxide pipe 37 will be mixed, and the gas introduced into the mixing chamber 35 will hit the spiral tube 36, thereby breaking up the airflow to facilitate the mixing of different gases. At the same time, the carbon dioxide pipe 37 and the other gas pipes 38 are interconnected with the mixing chamber 35, so that different gases can be introduced into the mixing chamber 35 for mixing.

[0053] The regulated gas is introduced into the culture dish 16 through the air inlet pipe 28 by the air pump 34. The air inlet pipe 28 is connected to each culture dish 16 through a connecting branch pipe, and a control valve 29 is installed at the position where each branch pipe is connected to the culture dish 16. Therefore, it can be prevented that the gas inside the culture dish 16 flows back into the inside of the air inlet pipe 28 to cause gas pollution, and the amount of carbon dioxide that needs to be introduced into different culture dishes 16 can also be controlled. At the same time, four groups of culture dishes 16 are installed in an array above the support plate 10, so multiple culture dishes 16 can be used for cell culture at the same time.

[0054] During the culture process, different nutrients required for cell culture can be placed inside the liquid box 32, and then the liquid box 32 is passed into the culture dish 16 through the liquid inlet pipe 30 and the pump body 31 for cell culture. At the same time, a control valve 29 is installed at the connection between the liquid inlet pipe 30 and the culture dish 16 to control the type of nutrients passed into the liquid inlet pipe 30 by the liquid box 32. Then, a control valve 29 is also installed at the position of the liquid inlet pipe 30 close to the culture dish 16 to control the amount of nutrients passed in, so as to facilitate the control and adjustment of the culture environment inside the culture dish 16;

[0055] Finally, during the culture process, the waste gas generated by the cells inside the culture dish 16 is discharged through the exhaust pipe 26, and then the fan 3 rotates to suck the gas outside the device into the interior of the culture chamber 5 through the air inlet 2. In the process of blowing into the interior of the culture chamber 5, the heating tube 4 heats the inhaled air to increase the temperature of the air so that the temperature of the air inhaled into the device adapts to the temperature required for cell culture to avoid the influence of inappropriate temperature on cell culture. Then the inhaled air circulates inside the culture chamber 5 and is discharged from the device through the air outlet 6, so that the air inside the culture chamber 5 can circulate, and at the same time, the waste gas generated by the cultured cells inside the culture chamber 5 can be taken away to maintain the air quality inside the culture chamber 5.

[0056] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An intelligent chimeric cell culture chamber, comprising an outer chamber body (1) and a culture mechanism installed above the outer chamber body (1), characterized in that: The culture mechanism includes a mounting assembly; The installation assembly comprises an air inlet (2), the right rear side of the outer cabin (1) is provided with an air inlet (2), a fan (3) is installed in front of the air inlet (2) through an outer frame, a heating pipe (4) is installed in front of the fan (3), and air dispersion ports (39) with different directions are opened in front of the heating pipe (4), a culture cabin (5) is opened in front of the bottom of the outer cabin (1), an air outlet (6) is opened on the left rear side of the outer cabin (1), and slide grooves (7) are opened on the left and right sides of the inner wall of the culture cabin (5), a slider (8) is installed inside the slider (8), pulleys (9) are installed in an array on the upper and lower sides of the slider (8), and a support plate (10) is fixedly installed on the side of the slider (8) and a baffle (11) is fixedly installed in front of the front.

2. The intelligent chimeric cell culture chamber according to claim 1, characterized in that: The support plate (10) is provided with an installation groove (12) in its internal array, a clamping plate (13) is installed in the installation groove (12), a rotating shaft (14) is installed in the middle of the clamping plate (13), a spring (15) is fixedly installed at the bottom of the clamping plate (13), a culture dish (16) is installed on the right side of the clamping plate (13), a limiting plate (17) whose size matches that of the clamping plate (13) is installed at the bottom of the culture dish (16), a top cover (18) is installed on the top of the culture dish (16), four groups of buckles (19) are installed in the side array of the top cover (18), a clamping block (20) is installed at the position corresponding to the buckle (19) on the top of the culture dish (16), and the position between the clamping blocks (20) is fixed to the bottom of the culture dish (16). A limit buckle (21) is installed, a mounting thread (22) is provided at the center of the top cover (18), the internal mounting block (23) is installed on the side of the mounting thread (22) through a threaded structure, a bearing (24) is installed inside the internal mounting block (23), an inner plate (25) is installed inside the bearing (24), an air outlet pipe (26) is fixedly installed on the right side of the inner plate (25), a one-way valve (27) is installed inside the air outlet pipe (26), a liquid inlet pipe (30) is installed inside the inner plate (25) at a position symmetrical to the air outlet pipe (26), an air inlet pipe (28) is fixedly installed behind the air outlet pipe (26), and a control valve (29) is installed inside the air inlet pipe (28).

3. The intelligent chimeric cell culture chamber according to claim 2, characterized in that: A pump body (31) is fixedly installed on the top of the liquid inlet pipe (30), and the top of the pump body (31) is connected to a liquid box (32) through the liquid inlet pipe (30). Four groups of liquid boxes (32) are installed in an array. A liquid inlet (33) is installed on the top of the liquid box (32). An air pump (34) is installed on the top of the air inlet pipe (28). The other side of the air pump (34) is connected to a gas mixing chamber (35) through the air inlet pipe (28). A spiral tube (36) is fixedly installed inside the gas mixing chamber (35). The right side of the gas mixing chamber (35) is connected to a carbon dioxide gas pipe (37), and other gas gas pipes (38) are connected and installed at the bottom middle of the carbon dioxide gas pipe (37).

4. The intelligent chimeric cell culture chamber according to claim 1, characterized in that: The air dispersion ports (39) are distributed in an array in front of the heating tube (4); the opening directions of the air dispersion ports (39) are staggered; the air inlet (2) forms a flow structure with the air dispersion ports (39) and the air outlet (6); the support plate (10) forms a sliding structure with the slide groove (7) through the cooperation between the slider (8) and the pulley (9); the support plate (10) forms a detachable structure with the outer cabin (1) through the cooperation between the slider (8) and the slide groove (7).

5. The intelligent chimeric cell culture chamber according to claim 2, characterized in that: The culture dish (16) forms a detachable structure with the support plate (10) through the cooperation between the limiting plate (17) and the clamping plate (13); the clamping plate (13) forms a rotating structure with the support plate (10) through the rotating shaft (14); the rotating shaft (14) forms a pressing structure with the support plate (10) through the spring (15); and the clamping plate (13) presents a "C"-shaped structure.

6. The intelligent chimeric cell culture chamber according to claim 2, characterized in that: The top cover (18) forms a snap-fit ​​structure with the culture dish (16) through the cooperation between the snap buckle (19) and the snap block (20), and the sizes of the snap buckle (19) and the limit buckle (21) match each other.

7. The intelligent chimeric cell culture chamber according to claim 2, characterized in that: The internal mounting block (23) forms a detachable structure with the top cover (18) through the mounting thread (22), and the inner plate (25) forms a rotating structure with the internal mounting block (23) through the bearing (24).

8. The intelligent chimeric cell culture chamber according to claim 2, characterized in that: The culture dishes (16) are provided in four groups, and the four groups of culture dishes (16) are connected to the air pump (34) via an air inlet pipe (28), and a control valve (29) is installed at one end of the air inlet pipe (28) close to the culture dish (16).

9. The intelligent chimeric cell culture chamber according to claim 3, characterized in that: The four groups of liquid boxes (32) are connected to the culture dishes (16) via a liquid inlet pipe (30), one end of the liquid inlet pipe (30) connected to the liquid box (32) is installed with a control valve (29), the four groups of culture dishes (16) are connected to the pump body (31) via the liquid inlet pipe (30), and one end of the liquid inlet pipe (30) connected to the culture dishes (16) is installed with an air inlet pipe (28).

10. The intelligent chimeric cell culture chamber according to claim 4, characterized in that: The spiral tube (36) is fixedly installed inside the gas mixing chamber (35). The spiral tube (36) is of a spiral structure. The centers of the spiral tube (36) and the gas mixing chamber (35) coincide with each other. The carbon dioxide gas pipe (37) and other gas gas pipes (38) are fixed to each other. The carbon dioxide gas pipe (37) and other gas gas pipes (38) are in communication with the spiral tube (36). The liquid inlet pipe (30) and the gas inlet pipe (28) are both hoses.

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