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Quick African swine fever virus detection card and application thereof

A technology of African swine fever virus and detection card, which is applied in the field of rapid detection card of African swine fever virus, can solve the problems of difficult detection of African swine fever virus, and achieve the effect of intuitive result judgment, high sensitivity and easy judgment

Inactive Publication Date: 2019-05-21
河南中泽生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a quick detection card for African swine fever virus to solve the difficult technical problem of African swine fever virus detection

Method used

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  • Quick African swine fever virus detection card and application thereof
  • Quick African swine fever virus detection card and application thereof
  • Quick African swine fever virus detection card and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Preparation of African swine fever virus rapid detection card

[0048] 1. Codon modification

[0049] Based on the sequence of the Georgia 2008 strain published by GenBank (GenBank: MH910495.1), combined with the inventor’s practical research experience and considering the codon preference of the E. coli expression system, combined with the mRNA secondary structure to avoid hairpin structures, especially ribosome binding The RNA structure near the site is modified and optimized for codons. The modified nucleotide sequence is as shown in SEQ ID NO.1 of the p30 protein gene of ASFV, and the nucleotide sequence is as shown in SEQ ID NO.2 of ASFV. The p54 protein gene and nucleotide sequence are as shown in the p72 protein gene of ASFV shown in SEQ ID NO.3; the stability of mRNA and the translation efficiency of protein can be effectively improved through genetic modification, and finally the protein expression can be increased, which is a large amount of protein...

Embodiment 2

[0105] Example 2: Detection of African swine fever virus by using the African swine fever virus rapid detection card

[0106] 1. Preparation of Assay Sample Solutions

[0107] Collect different test samples such as pig serum, diseased (dead) pig tissue, feces and vaccines, etc., and add appropriate amount of PBS or water for simple suspension or grinding.

[0108] 2. Test card test

[0109] Drop the solution to be tested into the sample loading end of the colloidal gold immunochromatography test strip; place it horizontally for 3 to 6 minutes to observe the results.

[0110] 3. Judgment of test results

[0111] The test card shows two reddish-brown bands (both test line and quality control line) "||", indicating that the sample to be tested contains ASFV antigen; only one reddish-brown band (quality control line) "|" is Negative means that no ASFV p30 protein, p54 protein or p72 protein antigen was detected in the sample to be tested; no bands appeared on the test card, ind...

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Abstract

The invention discloses a quick African swine fever virus detection card and application thereof, so as to solve the technical problem of hard African swine fever virus detection. Polyclonal antibodies PoAbI against African swine fever virus p30, P54 and p72 protein labeled with colloidal gold are absorbed on a colloidal gold labeling pad of the detection card; a detection membrane is provided with goat or rabbit anti-mouse IgG antibodies or a quality control line C printed by staphylococcus aureus SPA; and a detection line T printed by polyclonal antibodies PoAbII against African swine fevervirus p30, P54 and p72 protein is also arranged. The invention also discloses a using method for the quick African swine fever virus detection card. The method comprises steps: a to-be-detected sampleis acquired, and PBS or water is added for suspension or grinding; the sample is dripped to the sample loading end of the quick African swine fever virus detection card; and horizontal placing is carried out to observe a result. The quick African swine fever virus detection card adopts a double antibody sandwich method, and has the advantages of strong specificity, high sensitivity, high detection efficiency, high efficiency and practicability, and important practical application value.

Description

technical field [0001] The invention relates to the technical field of immune detection, in particular to a rapid detection card for African swine fever virus and its application. Background technique [0002] African swine fever (ASF) is a severe infectious disease caused by African swine fever virus (ASFV), which mainly infects pigs such as domestic pigs, wild boars, and bush pigs, and has a high mortality rate. cause huge economic losses. ASFV is the only DNA arbovirus known so far. ASFV only infects domestic pigs and wild boars, but not humans. Wild boars are usually recessively infected after ASFV infection. In addition, soft ticks are also the host and transmission medium of ASFV. ASFV was first discovered in Kenya in 1921, and then ASFV was found in many countries in southern and eastern Africa. At 17:00 on August 2, 2018, China Center for Animal Health and Epidemiology diagnosed a suspected African swine fever epidemic in Wuwu Community, Shenbei Street (Xinchengzi)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/58G01N33/68G01N33/558G01N33/532
Inventor 王爱萍陈玉梅赵建国刘运超邢广旭孙亚宁冯景
Owner 河南中泽生物工程有限公司
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